Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study
Background and purpose: Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles. Experimental approach: To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was d...
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2024-01-01
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| Series: | ADMET and DMPK |
| Subjects: | |
| Online Access: | https://pub.iapchem.org/ojs/index.php/admet/article/view/2102 |
| _version_ | 1797353694875877376 |
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| author | Sakthi Devi R Agnishwar Girigoswami Shanmugaraja Meenakshi Balasubramanian Deepika Karthick Harini Pemula Gowtham Pragya Pallavi Koyeli Girigoswami |
| author_facet | Sakthi Devi R Agnishwar Girigoswami Shanmugaraja Meenakshi Balasubramanian Deepika Karthick Harini Pemula Gowtham Pragya Pallavi Koyeli Girigoswami |
| author_sort | Sakthi Devi R |
| collection | DOAJ |
| description |
Background and purpose: Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles. Experimental approach: To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was done with chemically synthesized silver nanoparticles (AgNPs) and green tea mediated synthesis (GT/AgNP) in both in vitro using human alveolar cancer cell line (A549) and normal cell line (L132), and in in vivo with zebrafish embryos. Key results: The in vitro studies revealed that GT/AgNPs were less toxic to normal cells than cancer cells. The GT/AgNPs showed high biocompatibility for zebrafish embryos monitored microscopically for their developmental stages and by cumulative hatchability studies. The reduced hatchability found in the AgNPs-treated group was correlated by differential gene expression of zebrafish hatching enzymes (ZHE) (ZHE1 and ZHE2). Conclusion: The results indicated that nanoparticles can affect the hatching of zebrafish embryos and elicit toxicity at the gene level.
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| first_indexed | 2024-03-08T13:33:48Z |
| format | Article |
| id | doaj.art-d3916bedcfed494b8ad062f2a852f391 |
| institution | Directory Open Access Journal |
| issn | 1848-7718 |
| language | English |
| last_indexed | 2024-03-08T13:33:48Z |
| publishDate | 2024-01-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | ADMET and DMPK |
| spelling | doaj.art-d3916bedcfed494b8ad062f2a852f3912024-01-17T02:14:14ZengInternational Association of Physical Chemists (IAPC)ADMET and DMPK1848-77182024-01-0110.5599/admet.2102Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression studySakthi Devi R0Agnishwar Girigoswami1Shanmugaraja Meenakshi2Balasubramanian Deepika3Karthick Harini4Pemula Gowtham5Pragya Pallavi6Koyeli Girigoswami7Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, IndiaMedical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603 103, India Background and purpose: Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles. Experimental approach: To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was done with chemically synthesized silver nanoparticles (AgNPs) and green tea mediated synthesis (GT/AgNP) in both in vitro using human alveolar cancer cell line (A549) and normal cell line (L132), and in in vivo with zebrafish embryos. Key results: The in vitro studies revealed that GT/AgNPs were less toxic to normal cells than cancer cells. The GT/AgNPs showed high biocompatibility for zebrafish embryos monitored microscopically for their developmental stages and by cumulative hatchability studies. The reduced hatchability found in the AgNPs-treated group was correlated by differential gene expression of zebrafish hatching enzymes (ZHE) (ZHE1 and ZHE2). Conclusion: The results indicated that nanoparticles can affect the hatching of zebrafish embryos and elicit toxicity at the gene level. . https://pub.iapchem.org/ojs/index.php/admet/article/view/2102green synthesisbiocompatibilitynanoparticle toxicityfish embryozebrafish hatching (ZHE1 and ZHE2) |
| spellingShingle | Sakthi Devi R Agnishwar Girigoswami Shanmugaraja Meenakshi Balasubramanian Deepika Karthick Harini Pemula Gowtham Pragya Pallavi Koyeli Girigoswami Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study ADMET and DMPK green synthesis biocompatibility nanoparticle toxicity fish embryo zebrafish hatching (ZHE1 and ZHE2) |
| title | Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| title_full | Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| title_fullStr | Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| title_full_unstemmed | Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| title_short | Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| title_sort | beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study |
| topic | green synthesis biocompatibility nanoparticle toxicity fish embryo zebrafish hatching (ZHE1 and ZHE2) |
| url | https://pub.iapchem.org/ojs/index.php/admet/article/view/2102 |
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