ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells
Background/purpose: 5-Aminolevulinic acid mediated photodynamic therapy (ALA-PDT) has been used for the treatment of precancerous lesions and oral cancers. Compared with traditional treatment such as surgery, ALA-PDT can selectively kill cancer cells and reduce side effects. However, some cells migh...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-03-01
|
| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790213001694 |
| _version_ | 1818530764221841408 |
|---|---|
| author | Pei-Tzu Li En-Sheng Ke Pei-Chi Chiang Tsuimin Tsai |
| author_facet | Pei-Tzu Li En-Sheng Ke Pei-Chi Chiang Tsuimin Tsai |
| author_sort | Pei-Tzu Li |
| collection | DOAJ |
| description | Background/purpose: 5-Aminolevulinic acid mediated photodynamic therapy (ALA-PDT) has been used for the treatment of precancerous lesions and oral cancers. Compared with traditional treatment such as surgery, ALA-PDT can selectively kill cancer cells and reduce side effects. However, some cells might survive from PDT and may be directly attributable to the limited penetration of light. Therefore, it is necessary to elucidate alterations in cellular behavior and molecular mechanisms in cancer cells that have survived from PDT.
Materials and methods: Human tongue squamous carcinoma cells, SCC-4 cells, and mice bearing C26 tumors were used as cancer models in vitro and in vivo. After irradiation with the LD50 (50% lethal dose) light dose, the ability of cellular migration and metastasis related gene expression were assayed.
Results: Cells were rounded up and migration ability was shown to have significantly decreased under the influence of irradiation with the LD50 light dose. Gene expression related to metastasis including matrix metalloproteinases (MMPs) and chloride intracellular channel 4 (CLIC4) was also reduced. In addition, in the animal tumor model, mRNA expression of MMP9 and CLIC4 was also noticeably decreased.
Conclusion: Our results indicate that PDT could reduce the expression of invasion related genes of surviving cancer cells in vitro as well as in vivo. These could be used as references for clinical PDT of oral cancer in the future. |
| first_indexed | 2024-12-11T17:23:49Z |
| format | Article |
| id | doaj.art-d391cb6c7d5f44a5bf53c03cc08bdb5a |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-11T17:23:49Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-d391cb6c7d5f44a5bf53c03cc08bdb5a2022-12-22T00:57:02ZengElsevierJournal of Dental Sciences1991-79022015-03-01101748010.1016/j.jds.2013.10.005ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cellsPei-Tzu Li0En-Sheng Ke1Pei-Chi Chiang2Tsuimin Tsai3Graduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanTaipei Medical University–Shuang Ho Hospital, New Taipei City, TaiwanDepartment of Biochemical Science and Technology, National Taiwan University, Taipei, TaiwanGraduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanBackground/purpose: 5-Aminolevulinic acid mediated photodynamic therapy (ALA-PDT) has been used for the treatment of precancerous lesions and oral cancers. Compared with traditional treatment such as surgery, ALA-PDT can selectively kill cancer cells and reduce side effects. However, some cells might survive from PDT and may be directly attributable to the limited penetration of light. Therefore, it is necessary to elucidate alterations in cellular behavior and molecular mechanisms in cancer cells that have survived from PDT. Materials and methods: Human tongue squamous carcinoma cells, SCC-4 cells, and mice bearing C26 tumors were used as cancer models in vitro and in vivo. After irradiation with the LD50 (50% lethal dose) light dose, the ability of cellular migration and metastasis related gene expression were assayed. Results: Cells were rounded up and migration ability was shown to have significantly decreased under the influence of irradiation with the LD50 light dose. Gene expression related to metastasis including matrix metalloproteinases (MMPs) and chloride intracellular channel 4 (CLIC4) was also reduced. In addition, in the animal tumor model, mRNA expression of MMP9 and CLIC4 was also noticeably decreased. Conclusion: Our results indicate that PDT could reduce the expression of invasion related genes of surviving cancer cells in vitro as well as in vivo. These could be used as references for clinical PDT of oral cancer in the future.http://www.sciencedirect.com/science/article/pii/S1991790213001694ALA-PDTCe6-PDTCLIC4migration abilityMMP9 |
| spellingShingle | Pei-Tzu Li En-Sheng Ke Pei-Chi Chiang Tsuimin Tsai ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells Journal of Dental Sciences ALA-PDT Ce6-PDT CLIC4 migration ability MMP9 |
| title | ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells |
| title_full | ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells |
| title_fullStr | ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells |
| title_full_unstemmed | ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells |
| title_short | ALA- or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells |
| title_sort | ala or ce6 pdt induced phenotypic change and suppressed migration in surviving cancer cells |
| topic | ALA-PDT Ce6-PDT CLIC4 migration ability MMP9 |
| url | http://www.sciencedirect.com/science/article/pii/S1991790213001694 |
| work_keys_str_mv | AT peitzuli alaorce6pdtinducedphenotypicchangeandsuppressedmigrationinsurvivingcancercells AT enshengke alaorce6pdtinducedphenotypicchangeandsuppressedmigrationinsurvivingcancercells AT peichichiang alaorce6pdtinducedphenotypicchangeandsuppressedmigrationinsurvivingcancercells AT tsuimintsai alaorce6pdtinducedphenotypicchangeandsuppressedmigrationinsurvivingcancercells |