Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium
In order to solve the defects of traditional coal spontaneous combustion prevention technology in a closed goaf, a strain of aerobic endogenous bacteria was isolated from coal and used as a blocking raw material. Based on the metabolic and reproductive characteristics of microorganisms, the experime...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2571-6255/6/9/339 |
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| author | Xuanmeng Dong Fusheng Wang Liwen Guo Tiesheng Han |
| author_facet | Xuanmeng Dong Fusheng Wang Liwen Guo Tiesheng Han |
| author_sort | Xuanmeng Dong |
| collection | DOAJ |
| description | In order to solve the defects of traditional coal spontaneous combustion prevention technology in a closed goaf, a strain of aerobic endogenous bacteria was isolated from coal and used as a blocking raw material. Based on the metabolic and reproductive characteristics of microorganisms, the experimental study on the inhibition of coal spontaneous combustion by microorganisms was carried out. The colonies were isolated and purified by the dilution concentration plate method and the scribing plate method. The growth morphology of microorganisms was analyzed, and the growth curve was determined. The strains were identified by seamless cloning technology for high-throughput sequencing. The surface morphology of coal was analyzed by SEM, the differences of oxidation characteristic temperature points were analyzed by TG–DTG–DSC images, a programmed heating experiment was used to analyze the concentration of the indicator gas CO, and the changes in microscopic groups before and after microbial action were analyzed by FTIR and XPS spectra. Therefore, the inhibition of coal oxidation by endogenous bacteria was verified from macroscopic and microscopic perspectives. The results show that the coal bacteria isolated from the coal is <i>Lysinibacilus</i> sp. After the culture of <i>Lysinibacilus</i> sp., the surface of the coal demonstrated less detritus, and was relatively smooth. In the early stage of low temperature oxidation of coal spontaneous combustion, the characteristic temperature point of coal oxidation and the reaction between coal and O<sub>2</sub> could be delayed by <i>Lysinibacilus</i> sp., and the total heat release was reduced in the combustion process. Not only that, <i>Lysinibacilus</i> sp. could also reduce the CO concentration during coal heating. After the coal was decomposed by <i>Lysinibacilus</i> sp., the C=C thick ring skeleton structure had little effect; however, the aromatic substitution pattern changed. This bacterium had an effect on the C-O bond, reducing the percentage of -CH<sub>2</sub>- and increasing the percentage of -CH<sub>3</sub>. It might also use the crystalline water in coal for life activities. The carboxyl carbon in coal changed the most, with a decrease of 12.03%, so it might become the carbon source required for microbial growth. The reproductive metabolism of microorganisms also affected the form of nitrogen, and the percentage of pyridine nitrogen in coal was reduced. The ratio of single-bond carbon to double-bond carbon in raw coal was about 3:2, but after this bacterial action, the ratio of the two was about 1:1. The analytical conclusions of XPS and FTIR spectra were consistent, and the results supported each other. |
| first_indexed | 2024-03-10T22:46:41Z |
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| institution | Directory Open Access Journal |
| issn | 2571-6255 |
| language | English |
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| spelling | doaj.art-d39714163a2144d1ab9087a28219dd872023-11-19T10:40:07ZengMDPI AGFire2571-62552023-08-016933910.3390/fire6090339Study on the Influence of Coal Structure and Oxidation Performance by Endogenous BacteriumXuanmeng Dong0Fusheng Wang1Liwen Guo2Tiesheng Han3College of Mining Engineering, North China University of Science and Technology, Tangshan 063210, ChinaCollege of Mining Engineering, North China University of Science and Technology, Tangshan 063210, ChinaCollege of Mining Engineering, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaIn order to solve the defects of traditional coal spontaneous combustion prevention technology in a closed goaf, a strain of aerobic endogenous bacteria was isolated from coal and used as a blocking raw material. Based on the metabolic and reproductive characteristics of microorganisms, the experimental study on the inhibition of coal spontaneous combustion by microorganisms was carried out. The colonies were isolated and purified by the dilution concentration plate method and the scribing plate method. The growth morphology of microorganisms was analyzed, and the growth curve was determined. The strains were identified by seamless cloning technology for high-throughput sequencing. The surface morphology of coal was analyzed by SEM, the differences of oxidation characteristic temperature points were analyzed by TG–DTG–DSC images, a programmed heating experiment was used to analyze the concentration of the indicator gas CO, and the changes in microscopic groups before and after microbial action were analyzed by FTIR and XPS spectra. Therefore, the inhibition of coal oxidation by endogenous bacteria was verified from macroscopic and microscopic perspectives. The results show that the coal bacteria isolated from the coal is <i>Lysinibacilus</i> sp. After the culture of <i>Lysinibacilus</i> sp., the surface of the coal demonstrated less detritus, and was relatively smooth. In the early stage of low temperature oxidation of coal spontaneous combustion, the characteristic temperature point of coal oxidation and the reaction between coal and O<sub>2</sub> could be delayed by <i>Lysinibacilus</i> sp., and the total heat release was reduced in the combustion process. Not only that, <i>Lysinibacilus</i> sp. could also reduce the CO concentration during coal heating. After the coal was decomposed by <i>Lysinibacilus</i> sp., the C=C thick ring skeleton structure had little effect; however, the aromatic substitution pattern changed. This bacterium had an effect on the C-O bond, reducing the percentage of -CH<sub>2</sub>- and increasing the percentage of -CH<sub>3</sub>. It might also use the crystalline water in coal for life activities. The carboxyl carbon in coal changed the most, with a decrease of 12.03%, so it might become the carbon source required for microbial growth. The reproductive metabolism of microorganisms also affected the form of nitrogen, and the percentage of pyridine nitrogen in coal was reduced. The ratio of single-bond carbon to double-bond carbon in raw coal was about 3:2, but after this bacterial action, the ratio of the two was about 1:1. The analytical conclusions of XPS and FTIR spectra were consistent, and the results supported each other.https://www.mdpi.com/2571-6255/6/9/339microorganismcoal spontaneous combustioncoal structurecharacteristic temperaturelow-temperature oxidation |
| spellingShingle | Xuanmeng Dong Fusheng Wang Liwen Guo Tiesheng Han Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium Fire microorganism coal spontaneous combustion coal structure characteristic temperature low-temperature oxidation |
| title | Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium |
| title_full | Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium |
| title_fullStr | Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium |
| title_full_unstemmed | Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium |
| title_short | Study on the Influence of Coal Structure and Oxidation Performance by Endogenous Bacterium |
| title_sort | study on the influence of coal structure and oxidation performance by endogenous bacterium |
| topic | microorganism coal spontaneous combustion coal structure characteristic temperature low-temperature oxidation |
| url | https://www.mdpi.com/2571-6255/6/9/339 |
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