Measuring VLDL1 secretion in humans with an intravenous fat emulsion test
Summary: Tracer techniques to assess very-low-density lipoprotein (VLDL) secretion in humans are expensive, are time consuming, and require mathematical models to estimate VLDL kinetics. Here, we describe an alternative, time- and cost-efficient protocol to directly determine VLDL1 secretion with an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000473 |
| _version_ | 1811167266077671424 |
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| author | Matthäus Metz Clemens Baumgartner Herbert Stangl Thomas Scherer |
| author_facet | Matthäus Metz Clemens Baumgartner Herbert Stangl Thomas Scherer |
| author_sort | Matthäus Metz |
| collection | DOAJ |
| description | Summary: Tracer techniques to assess very-low-density lipoprotein (VLDL) secretion in humans are expensive, are time consuming, and require mathematical models to estimate VLDL kinetics. Here, we describe an alternative, time- and cost-efficient protocol to directly determine VLDL1 secretion with an intravenous (i.v.) lipid emulsion test that does not require tracers and compartmental modeling. We describe steps for intralipid infusion, blood sampling, and removal of intralipid from plasma samples, followed by density gradient ultracentrifugation to isolate VLDL1 fraction and measure the secretion rate.For complete details on the use and execution of this protocol, please refer to Bjorkegren et al. (1996),1 Al-Shayji et al. (2007),2 and Metz et al. (2022).3 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T16:07:34Z |
| format | Article |
| id | doaj.art-d397c16d5a7b4d4bb77343d1461a0c94 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T16:07:34Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-d397c16d5a7b4d4bb77343d1461a0c942023-02-10T04:23:22ZengElsevierSTAR Protocols2666-16672023-03-0141102089Measuring VLDL1 secretion in humans with an intravenous fat emulsion testMatthäus Metz0Clemens Baumgartner1Herbert Stangl2Thomas Scherer3Division of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University of Vienna, 1090 Vienna, Austria; Corresponding authorDivision of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna, 1090 Vienna, AustriaDivision of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University of Vienna, 1090 Vienna, Austria; Corresponding authorSummary: Tracer techniques to assess very-low-density lipoprotein (VLDL) secretion in humans are expensive, are time consuming, and require mathematical models to estimate VLDL kinetics. Here, we describe an alternative, time- and cost-efficient protocol to directly determine VLDL1 secretion with an intravenous (i.v.) lipid emulsion test that does not require tracers and compartmental modeling. We describe steps for intralipid infusion, blood sampling, and removal of intralipid from plasma samples, followed by density gradient ultracentrifugation to isolate VLDL1 fraction and measure the secretion rate.For complete details on the use and execution of this protocol, please refer to Bjorkegren et al. (1996),1 Al-Shayji et al. (2007),2 and Metz et al. (2022).3 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000473Clinical ProtocolMetabolismChemistry |
| spellingShingle | Matthäus Metz Clemens Baumgartner Herbert Stangl Thomas Scherer Measuring VLDL1 secretion in humans with an intravenous fat emulsion test STAR Protocols Clinical Protocol Metabolism Chemistry |
| title | Measuring VLDL1 secretion in humans with an intravenous fat emulsion test |
| title_full | Measuring VLDL1 secretion in humans with an intravenous fat emulsion test |
| title_fullStr | Measuring VLDL1 secretion in humans with an intravenous fat emulsion test |
| title_full_unstemmed | Measuring VLDL1 secretion in humans with an intravenous fat emulsion test |
| title_short | Measuring VLDL1 secretion in humans with an intravenous fat emulsion test |
| title_sort | measuring vldl1 secretion in humans with an intravenous fat emulsion test |
| topic | Clinical Protocol Metabolism Chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000473 |
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