Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae)
Abstract Bay snook (Petenia splendida) is a carnivorous cichlid species with excellent economic value in Southeast Mexico. Although this species presents an excellent potential for commercial aquaculture, the information about its nutritional, physiological, and reproductive metabolic pathways is me...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Ictiologia
2022-09-01
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| Series: | Neotropical Ichthyology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300204&tlng=en |
| _version_ | 1828176336230809600 |
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| author | Alejandra del Carmen Castillo-Collado Carlos Alfonso Frías-Quintana Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Gloria Asencio-Alcudia Emyr Saul Peña-Marín Gil Martínez-Bautista Luis Daniel Jiménez-Martinez Carlos Alfonso Álvarez-González |
| author_facet | Alejandra del Carmen Castillo-Collado Carlos Alfonso Frías-Quintana Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Gloria Asencio-Alcudia Emyr Saul Peña-Marín Gil Martínez-Bautista Luis Daniel Jiménez-Martinez Carlos Alfonso Álvarez-González |
| author_sort | Alejandra del Carmen Castillo-Collado |
| collection | DOAJ |
| description | Abstract Bay snook (Petenia splendida) is a carnivorous cichlid species with excellent economic value in Southeast Mexico. Although this species presents an excellent potential for commercial aquaculture, the information about its nutritional, physiological, and reproductive metabolic pathways is meager. The current study focuses on the expression of glucose transporter 2 (glut2) in embryos and larvae at 5, 10, 15-, 20-, 25-, and 30-days post-hatch (dph) and in the liver, intestine, kidney, muscle, heart, testicle, gill, stomach, pancreas, and brain of adult fish. The partial sequence of glut2 was obtained, and specific qPCR primers were designed. In embryos, the expression was lower compared to larvae at 5, 15, and 20 dph. The highest expression in larvae occurred at 20 dph and the lowest at 25 and 30 dph. Maximum expression levels in adults occurred in the liver and intestine. Our results show that glut2 is expressed differentially across tissues of adult bay snook, and it fluctuates during larval development. |
| first_indexed | 2024-04-12T04:42:33Z |
| format | Article |
| id | doaj.art-d399f8aa24604c62b4ab82a2867d4c2e |
| institution | Directory Open Access Journal |
| issn | 1982-0224 |
| language | English |
| last_indexed | 2024-04-12T04:42:33Z |
| publishDate | 2022-09-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj.art-d399f8aa24604c62b4ab82a2867d4c2e2022-12-22T03:47:35ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242022-09-0120310.1590/1982-0224-2021-0171Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae)Alejandra del Carmen Castillo-Colladohttps://orcid.org/0000-0003-0069-8456Carlos Alfonso Frías-Quintanahttps://orcid.org/0000-0002-5407-4991Vicente Morales-Garciahttps://orcid.org/0000-0002-7868-2529Carina Shianya Alvarez-Villagomezhttps://orcid.org/0000-0002-1606-9200Gloria Asencio-Alcudiahttps://orcid.org/0000-0002-7302-659XEmyr Saul Peña-Marínhttps://orcid.org/0000-0002-4736-9089Gil Martínez-Bautistahttps://orcid.org/0000-0001-5384-2461Luis Daniel Jiménez-Martinezhttps://orcid.org/0000-0001-6304-5669Carlos Alfonso Álvarez-Gonzálezhttps://orcid.org/0000-0001-9240-0041Abstract Bay snook (Petenia splendida) is a carnivorous cichlid species with excellent economic value in Southeast Mexico. Although this species presents an excellent potential for commercial aquaculture, the information about its nutritional, physiological, and reproductive metabolic pathways is meager. The current study focuses on the expression of glucose transporter 2 (glut2) in embryos and larvae at 5, 10, 15-, 20-, 25-, and 30-days post-hatch (dph) and in the liver, intestine, kidney, muscle, heart, testicle, gill, stomach, pancreas, and brain of adult fish. The partial sequence of glut2 was obtained, and specific qPCR primers were designed. In embryos, the expression was lower compared to larvae at 5, 15, and 20 dph. The highest expression in larvae occurred at 20 dph and the lowest at 25 and 30 dph. Maximum expression levels in adults occurred in the liver and intestine. Our results show that glut2 is expressed differentially across tissues of adult bay snook, and it fluctuates during larval development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300204&tlng=enAdult fishEarly ontogenyGene expressionGlucose transporterPetenia splendida |
| spellingShingle | Alejandra del Carmen Castillo-Collado Carlos Alfonso Frías-Quintana Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Gloria Asencio-Alcudia Emyr Saul Peña-Marín Gil Martínez-Bautista Luis Daniel Jiménez-Martinez Carlos Alfonso Álvarez-González Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) Neotropical Ichthyology Adult fish Early ontogeny Gene expression Glucose transporter Petenia splendida |
| title | Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_full | Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_fullStr | Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_full_unstemmed | Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_short | Characterization and expression of the gene glucose transporter 2 (GLUT2) in embryonic, larval and adult Bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_sort | characterization and expression of the gene glucose transporter 2 glut2 in embryonic larval and adult bay snook petenia splendida cichliformes cichlidae |
| topic | Adult fish Early ontogeny Gene expression Glucose transporter Petenia splendida |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300204&tlng=en |
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