Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach
In vitro anti-proliferative activity of <i>Pinus palustris</i> extract and its purified abietic acid was assessed against different human cancer cell lines (HepG-2, MCF-7 and HCT-116) compared to normal WI-38 cell line. Abietic acid showed more promising IC<sub>50</sub> value...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/1/293 |
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| author | Hesham Haffez Shimaa Osman Hassan Y. Ebrahim Zeinab A. Hassan |
| author_facet | Hesham Haffez Shimaa Osman Hassan Y. Ebrahim Zeinab A. Hassan |
| author_sort | Hesham Haffez |
| collection | DOAJ |
| description | In vitro anti-proliferative activity of <i>Pinus palustris</i> extract and its purified abietic acid was assessed against different human cancer cell lines (HepG-2, MCF-7 and HCT-116) compared to normal WI-38 cell line. Abietic acid showed more promising IC<sub>50</sub> values against MCF-7 cells than pine extract (0.06 µg/mL and 0.11 µM, respectively), with insignificant cytotoxicity toward normal fibroblast WI-38 cells. Abietic acid triggered both G<sub>2</sub>/M cell arrest and subG<sub>0</sub>-G<sub>1</sub> subpopulation in MCF-7, compared to SubG<sub>0</sub>-G<sub>1</sub> subpopulation arrest only for the extract. It also induced overexpression of key apoptotic genes (<i>Fas, FasL, Casp3, Casp8, Cyt-C</i> and <i>Bax</i>) and downregulation of both proliferation (<i>VEGF, IGFR1, TGF-β</i>) and oncogenic (<i>C-myc</i> and <i>NF-κB</i>) genes. Additionally, abietic acid induced overexpression of cytochrome-C protein. Furthermore, it increased levels of total antioxidants to diminish carcinogenesis and chemotherapy resistance. <i>P. palustris</i> is a valuable source of active abietic acid, an antiproliferative agent to MCF-7 cells through induction of apoptosis with promising future anticancer agency in breast cancer therapy. |
| first_indexed | 2024-03-10T03:30:18Z |
| format | Article |
| id | doaj.art-d39b5a6e3ada4de489b97b4a53089668 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:30:18Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d39b5a6e3ada4de489b97b4a530896682023-11-23T11:59:33ZengMDPI AGMolecules1420-30492022-01-0127129310.3390/molecules27010293Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro ApproachHesham Haffez0Shimaa Osman1Hassan Y. Ebrahim2Zeinab A. Hassan3Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Cairo 11795, EgyptHelwan General Hospital, Cairo 11731, EgyptPharmacognosy Department, Faculty of Pharmacy, Helwan University, Cairo 11795, EgyptBiochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Cairo 11795, EgyptIn vitro anti-proliferative activity of <i>Pinus palustris</i> extract and its purified abietic acid was assessed against different human cancer cell lines (HepG-2, MCF-7 and HCT-116) compared to normal WI-38 cell line. Abietic acid showed more promising IC<sub>50</sub> values against MCF-7 cells than pine extract (0.06 µg/mL and 0.11 µM, respectively), with insignificant cytotoxicity toward normal fibroblast WI-38 cells. Abietic acid triggered both G<sub>2</sub>/M cell arrest and subG<sub>0</sub>-G<sub>1</sub> subpopulation in MCF-7, compared to SubG<sub>0</sub>-G<sub>1</sub> subpopulation arrest only for the extract. It also induced overexpression of key apoptotic genes (<i>Fas, FasL, Casp3, Casp8, Cyt-C</i> and <i>Bax</i>) and downregulation of both proliferation (<i>VEGF, IGFR1, TGF-β</i>) and oncogenic (<i>C-myc</i> and <i>NF-κB</i>) genes. Additionally, abietic acid induced overexpression of cytochrome-C protein. Furthermore, it increased levels of total antioxidants to diminish carcinogenesis and chemotherapy resistance. <i>P. palustris</i> is a valuable source of active abietic acid, an antiproliferative agent to MCF-7 cells through induction of apoptosis with promising future anticancer agency in breast cancer therapy.https://www.mdpi.com/1420-3049/27/1/293abietic aidP. palustrisanti-cancerMCF-7apoptosis |
| spellingShingle | Hesham Haffez Shimaa Osman Hassan Y. Ebrahim Zeinab A. Hassan Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach Molecules abietic aid P. palustris anti-cancer MCF-7 apoptosis |
| title | Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach |
| title_full | Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach |
| title_fullStr | Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach |
| title_full_unstemmed | Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach |
| title_short | Growth Inhibition and Apoptotic Effect of Pine Extract and Abietic Acid on MCF-7 Breast Cancer Cells via Alteration of Multiple Gene Expressions Using In Vitro Approach |
| title_sort | growth inhibition and apoptotic effect of pine extract and abietic acid on mcf 7 breast cancer cells via alteration of multiple gene expressions using in vitro approach |
| topic | abietic aid P. palustris anti-cancer MCF-7 apoptosis |
| url | https://www.mdpi.com/1420-3049/27/1/293 |
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