Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering
The use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migratio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/14/3858 |
| _version_ | 1797526748663906304 |
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| author | Katarzyna Gębczak Benita Wiatrak Wojciech Fortuna |
| author_facet | Katarzyna Gębczak Benita Wiatrak Wojciech Fortuna |
| author_sort | Katarzyna Gębczak |
| collection | DOAJ |
| description | The use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migration of rat pheochromocytoma cell line PC12. PC12 cells were plated directly onto a CorMatrix flake or the well surface of a 12-well plate and cultured in RPMI-1640 medium and a medium supplemented with the nerve growth factor (NGF). The surface of the culture plates was modified with collagen type I (Col I). The number of PC12 cells was counted at four time points and then analysed for apoptosis using a staining kit containing annexin V conjugate with fluorescein and propidium iodide (PI). The effect of CorMatrix bioscaffold on the proliferation and migration of PC12 cells was tested by staining the cells with Hoechst 33258 solution for analysis using fluorescence microscopy. The research showed that the percentage of apoptotic and necrotic cells was low (less than 7%). CorMatrix stimulates the proliferation and possibly migration of PC12 cells that populate all levels of the three-dimensional architecture of the biomaterial. Further research on the mechanical and biochemical capabilities of CorMatrix offers prospects for the use of this material in neuro-regenerative applications. |
| first_indexed | 2024-03-10T09:33:47Z |
| format | Article |
| id | doaj.art-d3a0e4c0c7094b6ca21a17b21c57863b |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:33:47Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-d3a0e4c0c7094b6ca21a17b21c57863b2023-11-22T04:15:55ZengMDPI AGMaterials1996-19442021-07-011414385810.3390/ma14143858Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue EngineeringKatarzyna Gębczak0Benita Wiatrak1Wojciech Fortuna2Department of Basic Medical Sciences, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, PolandDepartment of Basic Medical Sciences, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, PolandDepartment of Neurosurgery, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, PolandThe use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migration of rat pheochromocytoma cell line PC12. PC12 cells were plated directly onto a CorMatrix flake or the well surface of a 12-well plate and cultured in RPMI-1640 medium and a medium supplemented with the nerve growth factor (NGF). The surface of the culture plates was modified with collagen type I (Col I). The number of PC12 cells was counted at four time points and then analysed for apoptosis using a staining kit containing annexin V conjugate with fluorescein and propidium iodide (PI). The effect of CorMatrix bioscaffold on the proliferation and migration of PC12 cells was tested by staining the cells with Hoechst 33258 solution for analysis using fluorescence microscopy. The research showed that the percentage of apoptotic and necrotic cells was low (less than 7%). CorMatrix stimulates the proliferation and possibly migration of PC12 cells that populate all levels of the three-dimensional architecture of the biomaterial. Further research on the mechanical and biochemical capabilities of CorMatrix offers prospects for the use of this material in neuro-regenerative applications.https://www.mdpi.com/1996-1944/14/14/3858CorMatrixbioscaffoldPC12 cells |
| spellingShingle | Katarzyna Gębczak Benita Wiatrak Wojciech Fortuna Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering Materials CorMatrix bioscaffold PC12 cells |
| title | Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering |
| title_full | Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering |
| title_fullStr | Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering |
| title_full_unstemmed | Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering |
| title_short | Evaluation of PC12 Cells’ Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering |
| title_sort | evaluation of pc12 cells proliferation adhesion and migration with the use of an extracellular matrix cormatrix for application in neural tissue engineering |
| topic | CorMatrix bioscaffold PC12 cells |
| url | https://www.mdpi.com/1996-1944/14/14/3858 |
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