Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators
Abstract Many conjunctival inflammatory diseases differ between the sexes and altered conjunctival goblet cells (CGCs) response is often involved. Inflammation is initiated by the release of pro-inflammatory mediators and terminated by the biosynthesis of specialized pro-resolution mediators (SPMs)....
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| Format: | Article |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20177-9 |
| _version_ | 1797998674631983104 |
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| author | Menglu Yang Haakon K. Fjærvoll Ketil A. Fjærvoll Nicholas H. Wang Tor P. Utheim Charles N. Serhan Darlene A. Dartt |
| author_facet | Menglu Yang Haakon K. Fjærvoll Ketil A. Fjærvoll Nicholas H. Wang Tor P. Utheim Charles N. Serhan Darlene A. Dartt |
| author_sort | Menglu Yang |
| collection | DOAJ |
| description | Abstract Many conjunctival inflammatory diseases differ between the sexes and altered conjunctival goblet cells (CGCs) response is often involved. Inflammation is initiated by the release of pro-inflammatory mediators and terminated by the biosynthesis of specialized pro-resolution mediators (SPMs). Herein, we determined the sex-based difference in the responses of CGCs to inflammatory stimuli or pro-resolving lipid SPMs and their interaction with sex hormones. GCs were cultured from pieces of human conjunctiva in RPMI media. CGCs were transferred 24 h before the start of experiments to phenol red-free and FBS-free media to minimize exogenous hormones. RT-PCR, immunofluorescence microscopy (IF), and Western Blot (WB) were performed to determine the presence of sex hormone receptors. Cellular response to pro-inflammatory stimuli or SPMs was studied by measuring the increase in intracellular [Ca2+] ([Ca2+]i) using fura 2/AM microscopy. Use of RT-PCR demonstrated estrogen receptor (ER) α in 4/5 males and 3/3 females; ERβ in 2/4 males and 2/3 females; and androgen receptors (AR) in 3/3 male and 3/3 female CGCs. Positive immunoreactivity by IF and protein expression by WB was detected using antibodies for the ERα and ERβ in 3/3 males and 3/3 females, while AR were only present in males. Significantly different Ca2+ responses between sexes were found with carbachol only at 10–3 M, but not with histamine or leukotriene (LT) B4 at any concentration used. Incubation with dihydrotestosterone (DHT), estrone (E1), or estradiol (E2) at 10–7 M for 30 min significantly inhibited the LTB4-stimulated [Ca2+]i increase in male and female CGCs. Incubation with DHT, E1, and E2 overnight significantly inhibited the LTB4 response in females, while DHT and E2 significantly inhibited the LTB4 response in males. The SPM lipoxin A4 (LXA4) (10–9–10−8 M), but not the resolvins D1 or D2, induced an [Ca2+]i increase that was significantly higher in males compared to females. We conclude that male and female CGCs showed differences in the expression of sex hormone receptors. Treatment with sex hormones altered pro-inflammatory mediator LTB4-induced response. Males compared to females have a higher response to the ω-6-fatty acid derived SPM LXA4, indicating males may terminate inflammation in conjunctival goblet cells faster than females. |
| first_indexed | 2024-04-11T10:52:29Z |
| format | Article |
| id | doaj.art-d3a1cb28fa4e41598223cf9468a189bb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T10:52:29Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-d3a1cb28fa4e41598223cf9468a189bb2022-12-22T04:28:52ZengNature PortfolioScientific Reports2045-23222022-09-0112111310.1038/s41598-022-20177-9Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediatorsMenglu Yang0Haakon K. Fjærvoll1Ketil A. Fjærvoll2Nicholas H. Wang3Tor P. Utheim4Charles N. Serhan5Darlene A. Dartt6Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research InstituteDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research InstituteDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research InstituteDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research InstituteDepartment of Medical Biochemistry, Oslo University HospitalDepartment of Anesthesia, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women’s Hospital and Harvard Medical SchoolDepartment of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Schepens Eye Research InstituteAbstract Many conjunctival inflammatory diseases differ between the sexes and altered conjunctival goblet cells (CGCs) response is often involved. Inflammation is initiated by the release of pro-inflammatory mediators and terminated by the biosynthesis of specialized pro-resolution mediators (SPMs). Herein, we determined the sex-based difference in the responses of CGCs to inflammatory stimuli or pro-resolving lipid SPMs and their interaction with sex hormones. GCs were cultured from pieces of human conjunctiva in RPMI media. CGCs were transferred 24 h before the start of experiments to phenol red-free and FBS-free media to minimize exogenous hormones. RT-PCR, immunofluorescence microscopy (IF), and Western Blot (WB) were performed to determine the presence of sex hormone receptors. Cellular response to pro-inflammatory stimuli or SPMs was studied by measuring the increase in intracellular [Ca2+] ([Ca2+]i) using fura 2/AM microscopy. Use of RT-PCR demonstrated estrogen receptor (ER) α in 4/5 males and 3/3 females; ERβ in 2/4 males and 2/3 females; and androgen receptors (AR) in 3/3 male and 3/3 female CGCs. Positive immunoreactivity by IF and protein expression by WB was detected using antibodies for the ERα and ERβ in 3/3 males and 3/3 females, while AR were only present in males. Significantly different Ca2+ responses between sexes were found with carbachol only at 10–3 M, but not with histamine or leukotriene (LT) B4 at any concentration used. Incubation with dihydrotestosterone (DHT), estrone (E1), or estradiol (E2) at 10–7 M for 30 min significantly inhibited the LTB4-stimulated [Ca2+]i increase in male and female CGCs. Incubation with DHT, E1, and E2 overnight significantly inhibited the LTB4 response in females, while DHT and E2 significantly inhibited the LTB4 response in males. The SPM lipoxin A4 (LXA4) (10–9–10−8 M), but not the resolvins D1 or D2, induced an [Ca2+]i increase that was significantly higher in males compared to females. We conclude that male and female CGCs showed differences in the expression of sex hormone receptors. Treatment with sex hormones altered pro-inflammatory mediator LTB4-induced response. Males compared to females have a higher response to the ω-6-fatty acid derived SPM LXA4, indicating males may terminate inflammation in conjunctival goblet cells faster than females.https://doi.org/10.1038/s41598-022-20177-9 |
| spellingShingle | Menglu Yang Haakon K. Fjærvoll Ketil A. Fjærvoll Nicholas H. Wang Tor P. Utheim Charles N. Serhan Darlene A. Dartt Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators Scientific Reports |
| title | Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators |
| title_full | Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators |
| title_fullStr | Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators |
| title_full_unstemmed | Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators |
| title_short | Sex-based differences in conjunctival goblet cell responses to pro-inflammatory and pro-resolving mediators |
| title_sort | sex based differences in conjunctival goblet cell responses to pro inflammatory and pro resolving mediators |
| url | https://doi.org/10.1038/s41598-022-20177-9 |
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