MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle
Early pregnancy diagnosis is vital for economic sustainability of dairy farms and maintaining the reproductive efficiency of the herd. There are many techniques including progesterone assay, pregnancy specific proteins and interferon stimulated genes have been explored for early pregnancy diagnosis...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-10-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/138127 |
| _version_ | 1797665372109799424 |
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| author | M K SHUKLA SHAMBHAVI KOUSHLESH RANJAN AMAN KUMAR ATUL KUMAR VERMA ASHUTOSH TRIPATHI NEELESH KAPOOR SURESH KUMAR |
| author_facet | M K SHUKLA SHAMBHAVI KOUSHLESH RANJAN AMAN KUMAR ATUL KUMAR VERMA ASHUTOSH TRIPATHI NEELESH KAPOOR SURESH KUMAR |
| author_sort | M K SHUKLA |
| collection | DOAJ |
| description |
Early pregnancy diagnosis is vital for economic sustainability of dairy farms and maintaining the reproductive efficiency of the herd. There are many techniques including progesterone assay, pregnancy specific proteins and interferon stimulated genes have been explored for early pregnancy diagnosis but, they are associated with varying level of efficacy. In the present experiment, interferon stimulated gene (Myxovirus resistance gene 2/MX2) expression pattern was used as a potential biomarker for early pregnancy in cattle. The association of MX2 gene expression in relation to progesterone assay was studied to explore its potential use as biomarker of early pregnancy. The plasma progesterone concentration in conceived animals on day 7 (2.26±0.19 ng/ml), 17 (5.42±0.35 ng/ml) and 21(6.38±0.39 ng/ml) was recorded to be significantly higher as compared to respective values in non-conceived animals, i.e. 1.55±0.09 ng/ml, 4.14±0.14 ng/ml and 0.81±0.06 ng/ml. The sudden decrement in plasma progesterone concentration after day 17th discriminates conceived and non-conceived animals. MX2 expression levels were observed to spike in blood due to release of interferon tau (τ) after implantation of embryo. The relative mRNA expression of MX2 gene showed a 9.5 to 28.64-fold higher expression on 17 days post insemination in pregnant animals as compared to non-pregnant animals. Thus, MX2 gene can be used as a reliable biomarker for the early detection of pregnancy.
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| first_indexed | 2024-03-11T19:42:53Z |
| format | Article |
| id | doaj.art-d3a3247d35974e5abe5a5a51e91f2a64 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-11T19:42:53Z |
| publishDate | 2023-10-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-d3a3247d35974e5abe5a5a51e91f2a642023-10-06T07:27:14ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272023-10-01931010.56093/ijans.v93i10.138127MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattleM K SHUKLA0SHAMBHAVI1KOUSHLESH RANJAN2AMAN KUMAR3ATUL KUMAR VERMA4ASHUTOSH TRIPATHI5NEELESH KAPOOR6SURESH KUMAR7Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaSardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaSardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaLala Lajpat Rai University of Veterinary and Animal Science, Hisar, HaryanaSardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaSardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaSardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh 250 110 IndiaICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh Early pregnancy diagnosis is vital for economic sustainability of dairy farms and maintaining the reproductive efficiency of the herd. There are many techniques including progesterone assay, pregnancy specific proteins and interferon stimulated genes have been explored for early pregnancy diagnosis but, they are associated with varying level of efficacy. In the present experiment, interferon stimulated gene (Myxovirus resistance gene 2/MX2) expression pattern was used as a potential biomarker for early pregnancy in cattle. The association of MX2 gene expression in relation to progesterone assay was studied to explore its potential use as biomarker of early pregnancy. The plasma progesterone concentration in conceived animals on day 7 (2.26±0.19 ng/ml), 17 (5.42±0.35 ng/ml) and 21(6.38±0.39 ng/ml) was recorded to be significantly higher as compared to respective values in non-conceived animals, i.e. 1.55±0.09 ng/ml, 4.14±0.14 ng/ml and 0.81±0.06 ng/ml. The sudden decrement in plasma progesterone concentration after day 17th discriminates conceived and non-conceived animals. MX2 expression levels were observed to spike in blood due to release of interferon tau (τ) after implantation of embryo. The relative mRNA expression of MX2 gene showed a 9.5 to 28.64-fold higher expression on 17 days post insemination in pregnant animals as compared to non-pregnant animals. Thus, MX2 gene can be used as a reliable biomarker for the early detection of pregnancy. https://epubs.icar.org.in/index.php/IJAnS/article/view/138127Interferon tau, MX2 gene, Pregnancy diagnosis, Progesterone |
| spellingShingle | M K SHUKLA SHAMBHAVI KOUSHLESH RANJAN AMAN KUMAR ATUL KUMAR VERMA ASHUTOSH TRIPATHI NEELESH KAPOOR SURESH KUMAR MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle Indian Journal of Animal Sciences Interferon tau, MX2 gene, Pregnancy diagnosis, Progesterone |
| title | MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle |
| title_full | MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle |
| title_fullStr | MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle |
| title_full_unstemmed | MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle |
| title_short | MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle |
| title_sort | mx2 gene mrna expression as potential biomarker for early pregnancy diagnosis in cattle |
| topic | Interferon tau, MX2 gene, Pregnancy diagnosis, Progesterone |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/138127 |
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