Prevalence of Autoantibodies and HLA DR, DQ in Type 1 Diabetes Mellitus
Introduction: Type I diabetes Mellitus (T1DM) is caused by autoimmune destruction of β-cells of pancreas. Two forms of T1DM are known called as 1A (autoimmune) and 1B (idiopathic). Aim: Aim was to study the prevalence of Anti-TTG IgA, AntiTPO, GADA, ZnT8 and IA-2 autoantibodies and HLA DR and DQ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8163/18657_CE[Ra1]_F(GH)_PF1(ROAK)_PFA(AK)_PFA2(PRAK).pdf |
| Summary: | Introduction: Type I diabetes Mellitus (T1DM) is caused by
autoimmune destruction of β-cells of pancreas. Two forms
of T1DM are known called as 1A (autoimmune) and 1B
(idiopathic).
Aim: Aim was to study the prevalence of Anti-TTG IgA, AntiTPO, GADA, ZnT8 and IA-2 autoantibodies and HLA DR and DQ
genes and its diagnostic value in T1DM.
Materials and Methods: Thirty four T1DM patients, 59 type 2
diabetes mellitus (T2DM) patients and 28 healthy controls were
included in study. Antibodies levels were estimated by ELISA
and HLA typing was performed by SSP-PCR method.
Result: The prevalence of various autoantibodies in T1DM
were Anti-TTG 14.7%, Anti-TPO 17.65%, GADA 38.23%, ZnT8
11.76% and IA-2 5.88%. Only GADA and ZnT8 were significantly
positive in T1DM. GADA (66.67%) and ZnT8 (33.33%) positivity
was more in patients below 15 years age while levels of other
antibodies were higher after 15 years age. All autoantibodies
were detected in higher frequency in T1DM than in T2DM and
controls.
HLA DR and DQ typing showed highly significant increase in
DRB1*0301 (61.76%, p=0.00) and DQB1*0201 (64.71%, p=0.00)
in T1DM. Subjects with HLA DRB1*0301 and DQB1*0201 had
80-100% positive prevalence of GADA, ZnT8, IA-2, Anti-TTG
and Anti-TPO autoantibodies.
Conclusion: Combination of GADA antibody with DRB1 and
DQB1 estimation improved diagnosis of T1A than insulin antigen
specific antibodies alone. |
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| ISSN: | 2249-782X 0973-709X |