Storage protein profile and amino acid content in wild rice Oryza glumaepatula

- The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions...

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Main Authors: Karina Freire D'eça Nogueira Santos, Ricardo Diógenes Dias Silveira, Claudia Cristina Garcia Martin-Didonet, Claudio Brondani
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2013-01-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100009&lng=en&tlng=en
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author Karina Freire D'eça Nogueira Santos
Ricardo Diógenes Dias Silveira
Claudia Cristina Garcia Martin-Didonet
Claudio Brondani
author_facet Karina Freire D'eça Nogueira Santos
Ricardo Diógenes Dias Silveira
Claudia Cristina Garcia Martin-Didonet
Claudio Brondani
author_sort Karina Freire D'eça Nogueira Santos
collection DOAJ
description - The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.
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spelling doaj.art-d3a4cf62562c4583ae3f907949f58b172022-12-22T01:54:29ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212013-01-01481667210.1590/S0100-204X2013000100009S0100-204X2013000100009Storage protein profile and amino acid content in wild rice Oryza glumaepatulaKarina Freire D'eça Nogueira Santos0Ricardo Diógenes Dias Silveira1Claudia Cristina Garcia Martin-Didonet2Claudio Brondani3Universidade Federal de GoiásUniversidade Federal de GoiásUniversidade Estadual de GoiásEmbrapa- The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100009&lng=en&tlng=enOryza sativarecursos genéticosqualidade nutricionalmelhoramento de plantasSDS-Page
spellingShingle Karina Freire D'eça Nogueira Santos
Ricardo Diógenes Dias Silveira
Claudia Cristina Garcia Martin-Didonet
Claudio Brondani
Storage protein profile and amino acid content in wild rice Oryza glumaepatula
Pesquisa Agropecuária Brasileira
Oryza sativa
recursos genéticos
qualidade nutricional
melhoramento de plantas
SDS-Page
title Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_full Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_fullStr Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_full_unstemmed Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_short Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_sort storage protein profile and amino acid content in wild rice oryza glumaepatula
topic Oryza sativa
recursos genéticos
qualidade nutricional
melhoramento de plantas
SDS-Page
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100009&lng=en&tlng=en
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