Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner
Optical imaging is crucial for cell examination, but its instruments' bulky optics limit their flexibility, and a single wavelength provides limited sample information. This paper presents a fibre cantilever-based endoscopic scanner for multiwavelength reflectance confocal microscopy. The scann...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Alexandria Engineering Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S111001682400382X |
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| author | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Mohd Saiful Dzulkefly Zan Norhana Arsad Farinawati Yazid Ahmad Ashrif A. Bakar |
| author_facet | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Mohd Saiful Dzulkefly Zan Norhana Arsad Farinawati Yazid Ahmad Ashrif A. Bakar |
| author_sort | Yang Sing Leong |
| collection | DOAJ |
| description | Optical imaging is crucial for cell examination, but its instruments' bulky optics limit their flexibility, and a single wavelength provides limited sample information. This paper presents a fibre cantilever-based endoscopic scanner for multiwavelength reflectance confocal microscopy. The scanner utilises the dual-core fibre cantilever to produce Lissajous scans while accommodating two distinct wavelengths. Coloured surfaces were imaged at wavelengths of 520 nm, 635 nm and 850 nm, and verified with a colour chart and reflectance spectroscopy. Blue ink-stained plant stem cross-section samples were also imaged and compared. Furthermore, the scanner performed structure morphology imaging on cellular samples. The 520 nm and 635 nm wavelengths create low- and high-contrast images respectively, while the 850 nm wavelength delivers a sharper image while also capturing the surface profile of the target sample. The findings demonstrated the endoscopic scanner’s capability to extract information based on different wavelengths, providing a more diverse view of the sample and enhancing the surface profile. Using 635 nm and 850 nm, the reconstructed images have a contrast enhancement of 67.9% and 90.8% respectively from 520 nm. The smallest feature size measured on the cellular sample is 5 µm. The endoscopic scanner has the potential to study microfluidic and cell morphology. |
| first_indexed | 2024-04-24T08:14:22Z |
| format | Article |
| id | doaj.art-d3a7414308f5474c8ef11e97b3f68de3 |
| institution | Directory Open Access Journal |
| issn | 1110-0168 |
| language | English |
| last_indexed | 2025-03-21T05:32:46Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Alexandria Engineering Journal |
| spelling | doaj.art-d3a7414308f5474c8ef11e97b3f68de32024-07-25T04:51:06ZengElsevierAlexandria Engineering Journal1110-01682024-06-01971424Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scannerYang Sing Leong0Mohd Hadri Hafiz Mokhtar1Mohd Saiful Dzulkefly Zan2Norhana Arsad3Farinawati Yazid4Ahmad Ashrif A. Bakar5Department of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, MalaysiaDepartment of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia; Photonics Research Group, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia; Corresponding author at: Department of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia.Department of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia; Photonics Research Group, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, MalaysiaDepartment of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia; Photonics Research Group, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, MalaysiaDepartment of Family Oral Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDepartment of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia; Photonics Research Group, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, MalaysiaOptical imaging is crucial for cell examination, but its instruments' bulky optics limit their flexibility, and a single wavelength provides limited sample information. This paper presents a fibre cantilever-based endoscopic scanner for multiwavelength reflectance confocal microscopy. The scanner utilises the dual-core fibre cantilever to produce Lissajous scans while accommodating two distinct wavelengths. Coloured surfaces were imaged at wavelengths of 520 nm, 635 nm and 850 nm, and verified with a colour chart and reflectance spectroscopy. Blue ink-stained plant stem cross-section samples were also imaged and compared. Furthermore, the scanner performed structure morphology imaging on cellular samples. The 520 nm and 635 nm wavelengths create low- and high-contrast images respectively, while the 850 nm wavelength delivers a sharper image while also capturing the surface profile of the target sample. The findings demonstrated the endoscopic scanner’s capability to extract information based on different wavelengths, providing a more diverse view of the sample and enhancing the surface profile. Using 635 nm and 850 nm, the reconstructed images have a contrast enhancement of 67.9% and 90.8% respectively from 520 nm. The smallest feature size measured on the cellular sample is 5 µm. The endoscopic scanner has the potential to study microfluidic and cell morphology.http://www.sciencedirect.com/science/article/pii/S111001682400382XCellular imagingConfocal microscopyEndoscopic scannerMultiwavelength imagingOptical imaging |
| spellingShingle | Yang Sing Leong Mohd Hadri Hafiz Mokhtar Mohd Saiful Dzulkefly Zan Norhana Arsad Farinawati Yazid Ahmad Ashrif A. Bakar Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner Alexandria Engineering Journal Cellular imaging Confocal microscopy Endoscopic scanner Multiwavelength imaging Optical imaging |
| title | Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| title_full | Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| title_fullStr | Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| title_full_unstemmed | Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| title_short | Enhancement of in-vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| title_sort | enhancement of in vitro cellular structure morphology imaging using multiwavelength confocal endoscopic scanner |
| topic | Cellular imaging Confocal microscopy Endoscopic scanner Multiwavelength imaging Optical imaging |
| url | http://www.sciencedirect.com/science/article/pii/S111001682400382X |
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