Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans
IntroductionPhytophthora infestans, the causative agent of late blight disease, has gained notoriety for its destructive potential, leading to substantial losses in potato yields. Although conventional systemic fungicides have been shown to be effective in controlling plant pathogens, growing enviro...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1278538/full |
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| author | Amal Hari Amal Hari Amal Hari Ghizlane Echchgadda Fatima-Azzahra Darkaoui Noamane Taarji Nihad Sahri Nihad Sahri Mansour Sobeh Said Ezrari Salah-Eddine Laasli Meryem Benjelloun Rachid Lahlali Rachid Lahlali |
| author_facet | Amal Hari Amal Hari Amal Hari Ghizlane Echchgadda Fatima-Azzahra Darkaoui Noamane Taarji Nihad Sahri Nihad Sahri Mansour Sobeh Said Ezrari Salah-Eddine Laasli Meryem Benjelloun Rachid Lahlali Rachid Lahlali |
| author_sort | Amal Hari |
| collection | DOAJ |
| description | IntroductionPhytophthora infestans, the causative agent of late blight disease, has gained notoriety for its destructive potential, leading to substantial losses in potato yields. Although conventional systemic fungicides have been shown to be effective in controlling plant pathogens, growing environmental concerns have prompted the need for more integrated disease management approaches. Hence, in this study, the effectiveness of wild Origanum elongatum extracts as biopesticides was explored in controlling P. infestans and potentially mitigating its devastating impact in planta.MethodsThe aerial parts of O. elongatum were subjected to sequential extraction using water, hexane, chloroform, and methanol. The obtained extracts were tested in vitro through the poisoned food procedure for their capacity to obstruct P. infestans growth and to defeat potato blight severity in vivo. The phyto-contents (total phenolic content (TPC) and total flavonoid content (TFC)), as well as the antioxidant activities, were spectrophotometrically determined in all extracts, and the phytoconstituents of the most active extract (methanolic extract) were profiled via high-performance liquid chromatography–photodiode array–tandem mass spectrometry (HPLC–PDA–MS/MS).ResultsIn vitro, the complete inhibition rate of the P. infestans was obtained using the methanolic extract at 5 mg/mL, followed by the hexane and chloroform extracts at 10 mg/mL. Interestingly, complete inhibition of the pathogen was achieved upon the application of the aqueous extract at 10 mg/mL. In vivo, the aqueous extract at 25 mg/mL reduced the P. infestans severity rate to 27.25%, while the methanolic extract at 20 mg/mL led to the lowest severity rate. Moreover, the hexane and chloroform extracts impaired the pathogen severity rate to 50% and 41% using 20 mg/mL, respectively. The TPC and TFC in the extracts were variable with high concentrations detected in the methanolic extract with 485.42 mg GAE/g and 58.24 mg QE/g, respectively. In addition, the methanolic extract showed the highest antioxidant activities, while the chloroform extract exhibited the lowest activity. Liquid chromatography (LC)–MS/MS analysis of the methanol extract revealed 56 components from diverse classes. These included organic acids, phenolic acids, flavonoids, tannins, and coumarins.ConclusionThese findings suggest that O. elongatum could be investigated as a potential source of antifungal compounds targeting different phytopathogens. |
| first_indexed | 2024-03-08T11:29:27Z |
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| last_indexed | 2024-03-08T11:29:27Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-d3ad91d652974d85b1d274a45efd2bf12024-01-26T04:18:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011510.3389/fpls.2024.12785381278538Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestansAmal Hari0Amal Hari1Amal Hari2Ghizlane Echchgadda3Fatima-Azzahra Darkaoui4Noamane Taarji5Nihad Sahri6Nihad Sahri7Mansour Sobeh8Said Ezrari9Salah-Eddine Laasli10Meryem Benjelloun11Rachid Lahlali12Rachid Lahlali13Department of Plant Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknes, Meknes, MoroccoLaboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, Fez, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University, Ben-Guerir, MoroccoDepartment of Plant Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknes, Meknes, MoroccoDepartment of Plant Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknes, Meknes, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University, Ben-Guerir, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University, Ben-Guerir, MoroccoPhysio-Chemical Laboratory of Inorganic and Organic Materials (LPCMIO), Materials Science Center (MSC), Ecole Normale Supérieure, Mohammed V University in Rabat, Rabat, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University, Ben-Guerir, MoroccoMicrobiology Unit, Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Medicine and Pharmacy Oujda, University Mohammed Premier, Oujda, MoroccoDepartment of Plant Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknes, Meknes, MoroccoLaboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, Fez, MoroccoDepartment of Plant Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknes, Meknes, MoroccoAgroBioSciences Program, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University, Ben-Guerir, MoroccoIntroductionPhytophthora infestans, the causative agent of late blight disease, has gained notoriety for its destructive potential, leading to substantial losses in potato yields. Although conventional systemic fungicides have been shown to be effective in controlling plant pathogens, growing environmental concerns have prompted the need for more integrated disease management approaches. Hence, in this study, the effectiveness of wild Origanum elongatum extracts as biopesticides was explored in controlling P. infestans and potentially mitigating its devastating impact in planta.MethodsThe aerial parts of O. elongatum were subjected to sequential extraction using water, hexane, chloroform, and methanol. The obtained extracts were tested in vitro through the poisoned food procedure for their capacity to obstruct P. infestans growth and to defeat potato blight severity in vivo. The phyto-contents (total phenolic content (TPC) and total flavonoid content (TFC)), as well as the antioxidant activities, were spectrophotometrically determined in all extracts, and the phytoconstituents of the most active extract (methanolic extract) were profiled via high-performance liquid chromatography–photodiode array–tandem mass spectrometry (HPLC–PDA–MS/MS).ResultsIn vitro, the complete inhibition rate of the P. infestans was obtained using the methanolic extract at 5 mg/mL, followed by the hexane and chloroform extracts at 10 mg/mL. Interestingly, complete inhibition of the pathogen was achieved upon the application of the aqueous extract at 10 mg/mL. In vivo, the aqueous extract at 25 mg/mL reduced the P. infestans severity rate to 27.25%, while the methanolic extract at 20 mg/mL led to the lowest severity rate. Moreover, the hexane and chloroform extracts impaired the pathogen severity rate to 50% and 41% using 20 mg/mL, respectively. The TPC and TFC in the extracts were variable with high concentrations detected in the methanolic extract with 485.42 mg GAE/g and 58.24 mg QE/g, respectively. In addition, the methanolic extract showed the highest antioxidant activities, while the chloroform extract exhibited the lowest activity. Liquid chromatography (LC)–MS/MS analysis of the methanol extract revealed 56 components from diverse classes. These included organic acids, phenolic acids, flavonoids, tannins, and coumarins.ConclusionThese findings suggest that O. elongatum could be investigated as a potential source of antifungal compounds targeting different phytopathogens.https://www.frontiersin.org/articles/10.3389/fpls.2024.1278538/fullSolanum tuberosum L.Phytophthora infestansOriganum elongatumchemical compositionantifungal activity |
| spellingShingle | Amal Hari Amal Hari Amal Hari Ghizlane Echchgadda Fatima-Azzahra Darkaoui Noamane Taarji Nihad Sahri Nihad Sahri Mansour Sobeh Said Ezrari Salah-Eddine Laasli Meryem Benjelloun Rachid Lahlali Rachid Lahlali Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans Frontiers in Plant Science Solanum tuberosum L. Phytophthora infestans Origanum elongatum chemical composition antifungal activity |
| title | Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans |
| title_full | Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans |
| title_fullStr | Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans |
| title_full_unstemmed | Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans |
| title_short | Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans |
| title_sort | chemical composition antioxidant properties and antifungal activity of wild origanum elongatum extracts against phytophthora infestans |
| topic | Solanum tuberosum L. Phytophthora infestans Origanum elongatum chemical composition antifungal activity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1278538/full |
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