Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey
Objectives: Appropriate antibiotic therapy and prevention of cross-contamination are the most important subjects in tuberculosis (TB) control. The aim of this study was to investigate the major phylogenetic clades and transmission rate of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520302186 |
| _version_ | 1818921644224151552 |
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| author | Alper Karagoz Hidayet Tutun Levent Altintas Umit Alanbayi Dilara Yildirim Nadir Kocak |
| author_facet | Alper Karagoz Hidayet Tutun Levent Altintas Umit Alanbayi Dilara Yildirim Nadir Kocak |
| author_sort | Alper Karagoz |
| collection | DOAJ |
| description | Objectives: Appropriate antibiotic therapy and prevention of cross-contamination are the most important subjects in tuberculosis (TB) control. The aim of this study was to investigate the major phylogenetic clades and transmission rate of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates (n = 200) from patients with TB in Sivas and Konya Provinces of Turkey. Methods: The phylogenetic relationship among the isolates was investigated by spoligotyping method. In addition, the 24-locus mycobacterial interspersed repetitive unit–variable-number tandem repeat (MIRU-VNTR) typing method was used to reveal cross-contamination. Results: Spoligotyping revealed 13 different spoligotypes. A total of 188 strains (94.0%) were included in the cluster. The most prominent spoligofamily was the T family (43.0% of strains), followed by LAM (26.0%), H (8.0%), X and S (both 6.0%) and U (5.0%). Also, 12 strains (6.0%) belonged to the Beijing profile. MIRU-VNTR results showed 176 (88.0%) different genotypes among the isolates. In total, 24 strains (12.0%) were in the cluster. Conclusions: According to spoligotyping, there is a heterogeneous M. tuberculosis population in Turkey. MIRU-VNTR results showed that cross-contamination observed between MDR M. tuberculosis isolates in Turkey is controllable. |
| first_indexed | 2024-12-20T01:40:55Z |
| format | Article |
| id | doaj.art-d3aee9db06dc41c6be444f2a16bbdbf5 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-20T01:40:55Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-d3aee9db06dc41c6be444f2a16bbdbf52022-12-21T19:57:53ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-12-0123130134Molecular typing of drug-resistant Mycobacterium tuberculosis strains from TurkeyAlper Karagoz0Hidayet Tutun1Levent Altintas2Umit Alanbayi3Dilara Yildirim4Nadir Kocak5Department of Molecular Biology and Genetics, Uşak University, Uşak, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, TurkeyDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, 06110 Diskapi, Ankara, Turkey; Corresponding author.Microbiology Laboratory, Sivas Numune Hospital, Sivas, TurkeyMicrobiology Laboratory, Sivas Numune Hospital, Sivas, TurkeyFaculty of Medicine, Medical Genetics, Selçuk University, Konya, TurkeyObjectives: Appropriate antibiotic therapy and prevention of cross-contamination are the most important subjects in tuberculosis (TB) control. The aim of this study was to investigate the major phylogenetic clades and transmission rate of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates (n = 200) from patients with TB in Sivas and Konya Provinces of Turkey. Methods: The phylogenetic relationship among the isolates was investigated by spoligotyping method. In addition, the 24-locus mycobacterial interspersed repetitive unit–variable-number tandem repeat (MIRU-VNTR) typing method was used to reveal cross-contamination. Results: Spoligotyping revealed 13 different spoligotypes. A total of 188 strains (94.0%) were included in the cluster. The most prominent spoligofamily was the T family (43.0% of strains), followed by LAM (26.0%), H (8.0%), X and S (both 6.0%) and U (5.0%). Also, 12 strains (6.0%) belonged to the Beijing profile. MIRU-VNTR results showed 176 (88.0%) different genotypes among the isolates. In total, 24 strains (12.0%) were in the cluster. Conclusions: According to spoligotyping, there is a heterogeneous M. tuberculosis population in Turkey. MIRU-VNTR results showed that cross-contamination observed between MDR M. tuberculosis isolates in Turkey is controllable.http://www.sciencedirect.com/science/article/pii/S2213716520302186Drug resistanceGenetic diversityMIRU-VNTRMycobacterium tuberculosisSpoligotypingTurkey |
| spellingShingle | Alper Karagoz Hidayet Tutun Levent Altintas Umit Alanbayi Dilara Yildirim Nadir Kocak Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey Journal of Global Antimicrobial Resistance Drug resistance Genetic diversity MIRU-VNTR Mycobacterium tuberculosis Spoligotyping Turkey |
| title | Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey |
| title_full | Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey |
| title_fullStr | Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey |
| title_full_unstemmed | Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey |
| title_short | Molecular typing of drug-resistant Mycobacterium tuberculosis strains from Turkey |
| title_sort | molecular typing of drug resistant mycobacterium tuberculosis strains from turkey |
| topic | Drug resistance Genetic diversity MIRU-VNTR Mycobacterium tuberculosis Spoligotyping Turkey |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520302186 |
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