Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties
Brown planthopper biotype 1, 2, 3 and a representative field population are required for resistance screening of promising rice lines in Indonesia, but the current biotype stocks has shown deviation in virulence patterns. The objectives of this study were to develop a set of brown planthopper popula...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2021-09-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/33899 |
| _version_ | 1818883124023525376 |
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| author | Chaerani Ahmad Dadang Sutrisno Bahagiawati Muhamad Yunus |
| author_facet | Chaerani Ahmad Dadang Sutrisno Bahagiawati Muhamad Yunus |
| author_sort | Chaerani |
| collection | DOAJ |
| description | Brown planthopper biotype 1, 2, 3 and a representative field population are required for resistance screening of promising rice lines in Indonesia, but the current biotype stocks has shown deviation in virulence patterns. The objectives of this study were to develop a set of brown planthopper populations with differential virulence and to investigate their genetic variability using SSR marker. Females originated from two field populations were selected on variety Mudgo (carries Bph1 gene) or ASD7 (bph2 gene) using honeydew excretion as the virulence parameter. Selection cycles resulted in population T, M, A, and R, which was raised and adapted on variety TN1 (carries no Bph gene), Mudgo, ASD7, and Rathu Heenathi (Bph3, Bph17), respectively. Population R was the most virulent as expected and can be used to represent a field population, but the remaining populations still showed high virulence level. AMOVA and PCoA results based on analysis with 38 SSR primer pairs revealed partial genetic separation among populations, with population R was the most genetically distant from the remaining populations. The desired virulence character of the remaining populations is expected could be achieved after further selection and prolonged adaptation on their respective hosts. |
| first_indexed | 2024-12-19T15:28:39Z |
| format | Article |
| id | doaj.art-d3af8d54ff414f859e6a27763a61dbf0 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-19T15:28:39Z |
| publishDate | 2021-09-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-d3af8d54ff414f859e6a27763a61dbf02022-12-21T20:15:48ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942021-09-0128410.4308/hjb.28.4.293-303Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host VarietiesChaerani0Ahmad Dadang1Sutrisno2Bahagiawati3Muhamad Yunus4ICABIOGRADICABIOGRADICABIOGRADICABIOGRADICABIOGRADBrown planthopper biotype 1, 2, 3 and a representative field population are required for resistance screening of promising rice lines in Indonesia, but the current biotype stocks has shown deviation in virulence patterns. The objectives of this study were to develop a set of brown planthopper populations with differential virulence and to investigate their genetic variability using SSR marker. Females originated from two field populations were selected on variety Mudgo (carries Bph1 gene) or ASD7 (bph2 gene) using honeydew excretion as the virulence parameter. Selection cycles resulted in population T, M, A, and R, which was raised and adapted on variety TN1 (carries no Bph gene), Mudgo, ASD7, and Rathu Heenathi (Bph3, Bph17), respectively. Population R was the most virulent as expected and can be used to represent a field population, but the remaining populations still showed high virulence level. AMOVA and PCoA results based on analysis with 38 SSR primer pairs revealed partial genetic separation among populations, with population R was the most genetically distant from the remaining populations. The desired virulence character of the remaining populations is expected could be achieved after further selection and prolonged adaptation on their respective hosts.https://journal.ipb.ac.id/index.php/hayati/article/view/33899 |
| spellingShingle | Chaerani Ahmad Dadang Sutrisno Bahagiawati Muhamad Yunus Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties Hayati Journal of Biosciences |
| title | Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties |
| title_full | Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties |
| title_fullStr | Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties |
| title_full_unstemmed | Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties |
| title_short | Virulence and SSR Diversity of Brown Planthopper (Nilaparvata lugens) Adapted on Differential Rice Host Varieties |
| title_sort | virulence and ssr diversity of brown planthopper nilaparvata lugens adapted on differential rice host varieties |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/33899 |
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