Downregulation of MALAT1 in triple-negative breast cancer cells
Background: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Mea...
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Elsevier
2024-03-01
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Series: | Biochemistry and Biophysics Reports |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823001735 |
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author | Dagmara Klopotowska Janusz Matuszyk |
author_facet | Dagmara Klopotowska Janusz Matuszyk |
author_sort | Dagmara Klopotowska |
collection | DOAJ |
description | Background: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Measurement of normalized relative quantity (NRQ) of MALAT1 transcripts in cell lines representing triple-negative breast cancer (TNBC) and luminal breast cancer. Materials and methods: The studies were performed using cell lines representing luminal breast cancer (T47D, MCF-7), TNBC (MDA-MB-468, CAL-51, MDA-MB-231), and MCF-10A cell line of normal breast epithelial cells. Total RNA was isolated from six independent cell cultures of each line, treated with DNase I, and used to synthesize complementary DNA, which was used in quantitative real-time PCR (qPCR) assays. Four MALAT1 fragments and reference genes CCSER2, ANKRD17, PUM1, GAPDH were amplified. Results: Geometric means of the NRQ of MALAT1 in breast cancer cell lines had the shortest 95% confidence intervals when CCSER2 was used for normalization. MALAT1 major transcript levels thus estimated in TNBC cell lines were found to be statistically significantly reduced compared to levels in both MCF-10A cells and luminal breast cancer cell lines, while MALAT1 minority splice variants were found to be increased in almost all breast cancer cell lines. Conclusion: CCSER2-normalized qPCR results indicate MALAT1 downregulation in cell lines representing the more aggressive breast cancer subtype compared to both the normal breast epithelial cell line and the estrogen receptor-positive breast cancer cell lines. |
first_indexed | 2024-03-08T03:30:42Z |
format | Article |
id | doaj.art-d3afca66fbc348b0b6261dcd54e86a38 |
institution | Directory Open Access Journal |
issn | 2405-5808 |
language | English |
last_indexed | 2024-03-08T03:30:42Z |
publishDate | 2024-03-01 |
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series | Biochemistry and Biophysics Reports |
spelling | doaj.art-d3afca66fbc348b0b6261dcd54e86a382024-02-11T05:11:11ZengElsevierBiochemistry and Biophysics Reports2405-58082024-03-0137101592Downregulation of MALAT1 in triple-negative breast cancer cellsDagmara Klopotowska0Janusz Matuszyk1Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, PolandCorresponding author.; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, PolandBackground: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Measurement of normalized relative quantity (NRQ) of MALAT1 transcripts in cell lines representing triple-negative breast cancer (TNBC) and luminal breast cancer. Materials and methods: The studies were performed using cell lines representing luminal breast cancer (T47D, MCF-7), TNBC (MDA-MB-468, CAL-51, MDA-MB-231), and MCF-10A cell line of normal breast epithelial cells. Total RNA was isolated from six independent cell cultures of each line, treated with DNase I, and used to synthesize complementary DNA, which was used in quantitative real-time PCR (qPCR) assays. Four MALAT1 fragments and reference genes CCSER2, ANKRD17, PUM1, GAPDH were amplified. Results: Geometric means of the NRQ of MALAT1 in breast cancer cell lines had the shortest 95% confidence intervals when CCSER2 was used for normalization. MALAT1 major transcript levels thus estimated in TNBC cell lines were found to be statistically significantly reduced compared to levels in both MCF-10A cells and luminal breast cancer cell lines, while MALAT1 minority splice variants were found to be increased in almost all breast cancer cell lines. Conclusion: CCSER2-normalized qPCR results indicate MALAT1 downregulation in cell lines representing the more aggressive breast cancer subtype compared to both the normal breast epithelial cell line and the estrogen receptor-positive breast cancer cell lines.http://www.sciencedirect.com/science/article/pii/S2405580823001735Breast cancer cell linesNon-coding RNAlncRNAReal-time PCRReference gene |
spellingShingle | Dagmara Klopotowska Janusz Matuszyk Downregulation of MALAT1 in triple-negative breast cancer cells Biochemistry and Biophysics Reports Breast cancer cell lines Non-coding RNA lncRNA Real-time PCR Reference gene |
title | Downregulation of MALAT1 in triple-negative breast cancer cells |
title_full | Downregulation of MALAT1 in triple-negative breast cancer cells |
title_fullStr | Downregulation of MALAT1 in triple-negative breast cancer cells |
title_full_unstemmed | Downregulation of MALAT1 in triple-negative breast cancer cells |
title_short | Downregulation of MALAT1 in triple-negative breast cancer cells |
title_sort | downregulation of malat1 in triple negative breast cancer cells |
topic | Breast cancer cell lines Non-coding RNA lncRNA Real-time PCR Reference gene |
url | http://www.sciencedirect.com/science/article/pii/S2405580823001735 |
work_keys_str_mv | AT dagmaraklopotowska downregulationofmalat1intriplenegativebreastcancercells AT januszmatuszyk downregulationofmalat1intriplenegativebreastcancercells |