Downregulation of MALAT1 in triple-negative breast cancer cells

Background: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Mea...

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Main Authors: Dagmara Klopotowska, Janusz Matuszyk
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Biochemistry and Biophysics Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580823001735
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author Dagmara Klopotowska
Janusz Matuszyk
author_facet Dagmara Klopotowska
Janusz Matuszyk
author_sort Dagmara Klopotowska
collection DOAJ
description Background: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Measurement of normalized relative quantity (NRQ) of MALAT1 transcripts in cell lines representing triple-negative breast cancer (TNBC) and luminal breast cancer. Materials and methods: The studies were performed using cell lines representing luminal breast cancer (T47D, MCF-7), TNBC (MDA-MB-468, CAL-51, MDA-MB-231), and MCF-10A cell line of normal breast epithelial cells. Total RNA was isolated from six independent cell cultures of each line, treated with DNase I, and used to synthesize complementary DNA, which was used in quantitative real-time PCR (qPCR) assays. Four MALAT1 fragments and reference genes CCSER2, ANKRD17, PUM1, GAPDH were amplified. Results: Geometric means of the NRQ of MALAT1 in breast cancer cell lines had the shortest 95% confidence intervals when CCSER2 was used for normalization. MALAT1 major transcript levels thus estimated in TNBC cell lines were found to be statistically significantly reduced compared to levels in both MCF-10A cells and luminal breast cancer cell lines, while MALAT1 minority splice variants were found to be increased in almost all breast cancer cell lines. Conclusion: CCSER2-normalized qPCR results indicate MALAT1 downregulation in cell lines representing the more aggressive breast cancer subtype compared to both the normal breast epithelial cell line and the estrogen receptor-positive breast cancer cell lines.
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spelling doaj.art-d3afca66fbc348b0b6261dcd54e86a382024-02-11T05:11:11ZengElsevierBiochemistry and Biophysics Reports2405-58082024-03-0137101592Downregulation of MALAT1 in triple-negative breast cancer cellsDagmara Klopotowska0Janusz Matuszyk1Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, PolandCorresponding author.; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, PolandBackground: MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression. Aim: Measurement of normalized relative quantity (NRQ) of MALAT1 transcripts in cell lines representing triple-negative breast cancer (TNBC) and luminal breast cancer. Materials and methods: The studies were performed using cell lines representing luminal breast cancer (T47D, MCF-7), TNBC (MDA-MB-468, CAL-51, MDA-MB-231), and MCF-10A cell line of normal breast epithelial cells. Total RNA was isolated from six independent cell cultures of each line, treated with DNase I, and used to synthesize complementary DNA, which was used in quantitative real-time PCR (qPCR) assays. Four MALAT1 fragments and reference genes CCSER2, ANKRD17, PUM1, GAPDH were amplified. Results: Geometric means of the NRQ of MALAT1 in breast cancer cell lines had the shortest 95% confidence intervals when CCSER2 was used for normalization. MALAT1 major transcript levels thus estimated in TNBC cell lines were found to be statistically significantly reduced compared to levels in both MCF-10A cells and luminal breast cancer cell lines, while MALAT1 minority splice variants were found to be increased in almost all breast cancer cell lines. Conclusion: CCSER2-normalized qPCR results indicate MALAT1 downregulation in cell lines representing the more aggressive breast cancer subtype compared to both the normal breast epithelial cell line and the estrogen receptor-positive breast cancer cell lines.http://www.sciencedirect.com/science/article/pii/S2405580823001735Breast cancer cell linesNon-coding RNAlncRNAReal-time PCRReference gene
spellingShingle Dagmara Klopotowska
Janusz Matuszyk
Downregulation of MALAT1 in triple-negative breast cancer cells
Biochemistry and Biophysics Reports
Breast cancer cell lines
Non-coding RNA
lncRNA
Real-time PCR
Reference gene
title Downregulation of MALAT1 in triple-negative breast cancer cells
title_full Downregulation of MALAT1 in triple-negative breast cancer cells
title_fullStr Downregulation of MALAT1 in triple-negative breast cancer cells
title_full_unstemmed Downregulation of MALAT1 in triple-negative breast cancer cells
title_short Downregulation of MALAT1 in triple-negative breast cancer cells
title_sort downregulation of malat1 in triple negative breast cancer cells
topic Breast cancer cell lines
Non-coding RNA
lncRNA
Real-time PCR
Reference gene
url http://www.sciencedirect.com/science/article/pii/S2405580823001735
work_keys_str_mv AT dagmaraklopotowska downregulationofmalat1intriplenegativebreastcancercells
AT januszmatuszyk downregulationofmalat1intriplenegativebreastcancercells