Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains
Abstract This study was conducted to biosynthesize Fe3O4@Ag nanocomposite with Scenedesmus obliquus extract, and to evaluate its antibacterial potential against Pseudomonas aeruginosa. In addition, the effect of the synthesized nanocomposite on the expression of MexA‐B efflux pump genes was investig...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | Micro & Nano Letters |
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| Online Access: | https://doi.org/10.1049/mna2.12138 |
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| author | Sara Hadad Tabarek Abdulrazaq Alkinani Aida Mahmoudi Somayeh Dehghan Nastaran Maleki Moaf Akram Sadat Naeemi Ali Salehzadeh |
| author_facet | Sara Hadad Tabarek Abdulrazaq Alkinani Aida Mahmoudi Somayeh Dehghan Nastaran Maleki Moaf Akram Sadat Naeemi Ali Salehzadeh |
| author_sort | Sara Hadad |
| collection | DOAJ |
| description | Abstract This study was conducted to biosynthesize Fe3O4@Ag nanocomposite with Scenedesmus obliquus extract, and to evaluate its antibacterial potential against Pseudomonas aeruginosa. In addition, the effect of the synthesized nanocomposite on the expression of MexA‐B efflux pump genes was investigated. The Fe3O4@Ag nanocomposite was characterized using X‐ray diffraction spectroscopy (EDX), Fourier transform infrared spectroscopy (FT‐IR), transmission electron microscopy (TEM), Dynamic light scattering (DLS) as well as Zeta potential analyses. Minimum inhibitory concentration (MIC) of the nanocomposite alone and in combination with ciprofloxacin against P. aeruginosa strains was determined. Finally, the expression of MexA‐B efflux pump genes among the bacteria treated with the nanocomposite was evaluated using quantitative real‐time PCR. Based on the results, the proper synthesis of the nanocomposite with the diameter of 40 to 70 nm and acceptable stability was confirmed. The synthesized nanocomposite had lower MIC value (8–64 μg/mL) than ciprofloxacin (64–512 μg/mL) which shows stronger antibacterial potential of the nanocomposite. In addition, treating bacteria with the nanocomposite and ciprofloxacin caused a significant reduction of MexA‐B genes compared to the cells treated with ciprofloxacin or nanocomposite alone. Thus, Fe3O4@Ag nanocomposite can improve the efficacy of ciprofloxacin against P. aeruginosa by reducing the expression of MexA‐B efflux pump genes. |
| first_indexed | 2024-04-13T22:57:05Z |
| format | Article |
| id | doaj.art-d3b109793c2b4cb497a854276ad5ef87 |
| institution | Directory Open Access Journal |
| issn | 1750-0443 |
| language | English |
| last_indexed | 2024-04-13T22:57:05Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Micro & Nano Letters |
| spelling | doaj.art-d3b109793c2b4cb497a854276ad5ef872022-12-22T02:25:58ZengWileyMicro & Nano Letters1750-04432022-10-01171231932610.1049/mna2.12138Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strainsSara Hadad0Tabarek Abdulrazaq Alkinani1Aida Mahmoudi2Somayeh Dehghan3Nastaran Maleki Moaf4Akram Sadat Naeemi5Ali Salehzadeh6Department of Biology Rasht Branch Islamic Azad University Rasht IranDepartment of Biology, Science and Research Branch Islamic Azad University Tehran IranDepartment of Biology Damghan Branch Islamic Azad University Damghan IranDepartment of Biology Central Tehran Branch Islamic Azad University Tehran IranDepartment of Biology Lahijan Branch Islamic Azad University Lahijan IranDepartment of Biology Faculty of Science University of Guilan Rasht IranDepartment of Biology Rasht Branch Islamic Azad University Rasht IranAbstract This study was conducted to biosynthesize Fe3O4@Ag nanocomposite with Scenedesmus obliquus extract, and to evaluate its antibacterial potential against Pseudomonas aeruginosa. In addition, the effect of the synthesized nanocomposite on the expression of MexA‐B efflux pump genes was investigated. The Fe3O4@Ag nanocomposite was characterized using X‐ray diffraction spectroscopy (EDX), Fourier transform infrared spectroscopy (FT‐IR), transmission electron microscopy (TEM), Dynamic light scattering (DLS) as well as Zeta potential analyses. Minimum inhibitory concentration (MIC) of the nanocomposite alone and in combination with ciprofloxacin against P. aeruginosa strains was determined. Finally, the expression of MexA‐B efflux pump genes among the bacteria treated with the nanocomposite was evaluated using quantitative real‐time PCR. Based on the results, the proper synthesis of the nanocomposite with the diameter of 40 to 70 nm and acceptable stability was confirmed. The synthesized nanocomposite had lower MIC value (8–64 μg/mL) than ciprofloxacin (64–512 μg/mL) which shows stronger antibacterial potential of the nanocomposite. In addition, treating bacteria with the nanocomposite and ciprofloxacin caused a significant reduction of MexA‐B genes compared to the cells treated with ciprofloxacin or nanocomposite alone. Thus, Fe3O4@Ag nanocomposite can improve the efficacy of ciprofloxacin against P. aeruginosa by reducing the expression of MexA‐B efflux pump genes.https://doi.org/10.1049/mna2.12138CiprofloxacinEfflux pumpNanocompositePseudomonas aeruginosa |
| spellingShingle | Sara Hadad Tabarek Abdulrazaq Alkinani Aida Mahmoudi Somayeh Dehghan Nastaran Maleki Moaf Akram Sadat Naeemi Ali Salehzadeh Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains Micro & Nano Letters Ciprofloxacin Efflux pump Nanocomposite Pseudomonas aeruginosa |
| title | Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains |
| title_full | Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains |
| title_fullStr | Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains |
| title_full_unstemmed | Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains |
| title_short | Biological synthesis of Fe3O4@Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA‐B efflux pump genes in ciprofloxacin‐resistant Pseudomonas aeruginosa strains |
| title_sort | biological synthesis of fe3o4 ag using scenedesmus obliquus and evaluation of its effect on the expression of mexa b efflux pump genes in ciprofloxacin resistant pseudomonas aeruginosa strains |
| topic | Ciprofloxacin Efflux pump Nanocomposite Pseudomonas aeruginosa |
| url | https://doi.org/10.1049/mna2.12138 |
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