Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis
In juvenile Chinese mitten crabs, Eriocheir sinensis, the occurrence of sexual gland maturation in the first year is known as precocity and significantly affects culture productivity and profitability. The miniaturization of individuals is a key characteristic of precocious crabs. Most related studi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342200388X |
| _version_ | 1811209372307554304 |
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| author | Huan Liu Lei Guo Weiwei Zhang Jiahui Peng Qinna Chen Fang Cao Zhaohui Zhang Mingshen Guo Han Zhang Shumei Mu Xianjiang Kang |
| author_facet | Huan Liu Lei Guo Weiwei Zhang Jiahui Peng Qinna Chen Fang Cao Zhaohui Zhang Mingshen Guo Han Zhang Shumei Mu Xianjiang Kang |
| author_sort | Huan Liu |
| collection | DOAJ |
| description | In juvenile Chinese mitten crabs, Eriocheir sinensis, the occurrence of sexual gland maturation in the first year is known as precocity and significantly affects culture productivity and profitability. The miniaturization of individuals is a key characteristic of precocious crabs. Most related studies focus on the underlying causes, nutritional quality, biochemical components and differentially expressed mRNAs of precocious crab individuals and tissues. The reproductive performance of precocious crabs is evaluated based on offspring quality. However, the status of precocious parental gametes (especially spermatozoa) directly related to the offspring has not been reported. To clarify the differences in spermatozoa between precocious and normal mature crabs, spermatozoal transcriptome and proteome data were obtained via high-throughput sequencing technology. The results revealed a total of 856 differentially expressed genes and 150 differentially expressed proteins in spermatozoa. Functional analysis showed that these genes and proteins identified in the spermatozoa of precocious E. sinensis were significantly enriched in the categories of substance and energy metabolism. And more, the miniaturization of spermatogenic cells was also identified in precocious crabs. This indicates that metabolic disorders might be the main reason for the miniaturization of spermatogenic cells in precocious E. sinensis males. The molecular defects in precocious sperm were closely related to the imbalances in essential substance and energy metabolism. These findings provide foundational information and insights into hidden biological irregularities associated with precocious phenotypes and will contribute to the further study of precocious E. sinensis males and even other crustaceans. |
| first_indexed | 2024-04-12T04:38:21Z |
| format | Article |
| id | doaj.art-d3b364dac3f546efb57184e60114bf83 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-12T04:38:21Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-d3b364dac3f546efb57184e60114bf832022-12-22T03:47:45ZengElsevierAquaculture Reports2352-51342022-12-0127101392Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensisHuan Liu0Lei Guo1Weiwei Zhang2Jiahui Peng3Qinna Chen4Fang Cao5Zhaohui Zhang6Mingshen Guo7Han Zhang8Shumei Mu9Xianjiang Kang10College of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, ChinaSchool of Basic Medical Sciences, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, ChinaDepartment of Reproductive Medicine, Baoding First Central Hospital, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, China; Corresponding authors at: College of Life Science, Hebei University, Baoding 071000, ChinaCollege of Life Science, Hebei University, Baoding 071000, China; Institute of Life Sciences and Green Development, Hebei University, Baoding 071000, China; Hebei Innovation Center for Bioengineering and Biotechnology, Hebei University, Baoding 071000, China; Corresponding authors at: College of Life Science, Hebei University, Baoding 071000, ChinaIn juvenile Chinese mitten crabs, Eriocheir sinensis, the occurrence of sexual gland maturation in the first year is known as precocity and significantly affects culture productivity and profitability. The miniaturization of individuals is a key characteristic of precocious crabs. Most related studies focus on the underlying causes, nutritional quality, biochemical components and differentially expressed mRNAs of precocious crab individuals and tissues. The reproductive performance of precocious crabs is evaluated based on offspring quality. However, the status of precocious parental gametes (especially spermatozoa) directly related to the offspring has not been reported. To clarify the differences in spermatozoa between precocious and normal mature crabs, spermatozoal transcriptome and proteome data were obtained via high-throughput sequencing technology. The results revealed a total of 856 differentially expressed genes and 150 differentially expressed proteins in spermatozoa. Functional analysis showed that these genes and proteins identified in the spermatozoa of precocious E. sinensis were significantly enriched in the categories of substance and energy metabolism. And more, the miniaturization of spermatogenic cells was also identified in precocious crabs. This indicates that metabolic disorders might be the main reason for the miniaturization of spermatogenic cells in precocious E. sinensis males. The molecular defects in precocious sperm were closely related to the imbalances in essential substance and energy metabolism. These findings provide foundational information and insights into hidden biological irregularities associated with precocious phenotypes and will contribute to the further study of precocious E. sinensis males and even other crustaceans.http://www.sciencedirect.com/science/article/pii/S235251342200388XEriocheir sinensisPrecocious pubertySperm proteomeTranscriptomeAbnormal sperm metabolism |
| spellingShingle | Huan Liu Lei Guo Weiwei Zhang Jiahui Peng Qinna Chen Fang Cao Zhaohui Zhang Mingshen Guo Han Zhang Shumei Mu Xianjiang Kang Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis Aquaculture Reports Eriocheir sinensis Precocious puberty Sperm proteome Transcriptome Abnormal sperm metabolism |
| title | Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis |
| title_full | Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis |
| title_fullStr | Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis |
| title_full_unstemmed | Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis |
| title_short | Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis |
| title_sort | transcriptome and proteome reveal abnormal spermatozoa in precocious chinese mitten crab eriocheir sinensis |
| topic | Eriocheir sinensis Precocious puberty Sperm proteome Transcriptome Abnormal sperm metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S235251342200388X |
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