α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis
Abstract Objectives Unlike in adult rheumatology, for most forms of juvenile idiopathic arthritis (JIA) no reliable biomarkers currently exist to assess joint and disease activity. However, electrophoresis is frequently found changed in active juvenile arthritis. The objective of this study was to e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Pediatric Rheumatology Online Journal |
| Online Access: | https://doi.org/10.1186/s12969-022-00721-7 |
| _version_ | 1811320663527391232 |
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| author | Ludwig Zeller Pascal N. Tyrrell Stella Wang Nadine Fischer Johannes-Peter Haas Boris Hügle |
| author_facet | Ludwig Zeller Pascal N. Tyrrell Stella Wang Nadine Fischer Johannes-Peter Haas Boris Hügle |
| author_sort | Ludwig Zeller |
| collection | DOAJ |
| description | Abstract Objectives Unlike in adult rheumatology, for most forms of juvenile idiopathic arthritis (JIA) no reliable biomarkers currently exist to assess joint and disease activity. However, electrophoresis is frequently found changed in active juvenile arthritis. The objective of this study was to evaluate the α2-fraction of serum electrophoresis and its main components as biomarkers for JIA, categories extended/persistent oligoarthritis and seronegative polyarthritis, in comparison with the conventionally used erythrocyte sedimentation rate and C-reactive protein. Methods Serum samples and clinical data from 181 patients with JIA were collected. Serum electrophoresis and α2-fraction and its components were determined using standard methods. Relationship between calculated α2-fraction of serum electrophoresis (CA2F) and its components, acute-phase parameters and cJADAS27 was assessed using Pearson’s correlation coefficient and linear regression modelling, adjusting for confounding effects. Results were confirmed in a second cohort with 223 serum samples from 37 patients, using a mixed model to account for repeated measures. Results Compared to ESR and CRP, CA2F showed higher correlation to cJADAS27, in particular for persistent oligoarthritis. Of the three components of the α2-fraction, haptoglobin showed the highest correlation to cJADAS27. Regression analysis demonstrated higher ability to predict cJADAS27 for CA2F, and especially for haptoglobin as a component thereof, than for CRP and ESR. Conclusion Compared to conventional methods, α2-fraction of serum electrophoresis and specifically, haptoglobin show higher correlations with disease activity in common subtypes of JIA, representing excellent candidates as biomarkers for disease activity. Further studies are necessary to determine diagnostic value and correlations in other subtypes. |
| first_indexed | 2024-04-13T13:03:27Z |
| format | Article |
| id | doaj.art-d3b43f7965d341a196a51b1d35e36e88 |
| institution | Directory Open Access Journal |
| issn | 1546-0096 |
| language | English |
| last_indexed | 2024-04-13T13:03:27Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Pediatric Rheumatology Online Journal |
| spelling | doaj.art-d3b43f7965d341a196a51b1d35e36e882022-12-22T02:45:51ZengBMCPediatric Rheumatology Online Journal1546-00962022-08-012011910.1186/s12969-022-00721-7α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritisLudwig Zeller0Pascal N. Tyrrell1Stella Wang2Nadine Fischer3Johannes-Peter Haas4Boris Hügle5German Centre for Pediatric and Adolescent Rheumatology (GCPAR)Department of Medical Imaging, University of TorontoDepartment of Medical Imaging, University of TorontoGerman Centre for Pediatric and Adolescent Rheumatology (GCPAR)German Centre for Pediatric and Adolescent Rheumatology (GCPAR)German Centre for Pediatric and Adolescent Rheumatology (GCPAR)Abstract Objectives Unlike in adult rheumatology, for most forms of juvenile idiopathic arthritis (JIA) no reliable biomarkers currently exist to assess joint and disease activity. However, electrophoresis is frequently found changed in active juvenile arthritis. The objective of this study was to evaluate the α2-fraction of serum electrophoresis and its main components as biomarkers for JIA, categories extended/persistent oligoarthritis and seronegative polyarthritis, in comparison with the conventionally used erythrocyte sedimentation rate and C-reactive protein. Methods Serum samples and clinical data from 181 patients with JIA were collected. Serum electrophoresis and α2-fraction and its components were determined using standard methods. Relationship between calculated α2-fraction of serum electrophoresis (CA2F) and its components, acute-phase parameters and cJADAS27 was assessed using Pearson’s correlation coefficient and linear regression modelling, adjusting for confounding effects. Results were confirmed in a second cohort with 223 serum samples from 37 patients, using a mixed model to account for repeated measures. Results Compared to ESR and CRP, CA2F showed higher correlation to cJADAS27, in particular for persistent oligoarthritis. Of the three components of the α2-fraction, haptoglobin showed the highest correlation to cJADAS27. Regression analysis demonstrated higher ability to predict cJADAS27 for CA2F, and especially for haptoglobin as a component thereof, than for CRP and ESR. Conclusion Compared to conventional methods, α2-fraction of serum electrophoresis and specifically, haptoglobin show higher correlations with disease activity in common subtypes of JIA, representing excellent candidates as biomarkers for disease activity. Further studies are necessary to determine diagnostic value and correlations in other subtypes.https://doi.org/10.1186/s12969-022-00721-7 |
| spellingShingle | Ludwig Zeller Pascal N. Tyrrell Stella Wang Nadine Fischer Johannes-Peter Haas Boris Hügle α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis Pediatric Rheumatology Online Journal |
| title | α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis |
| title_full | α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis |
| title_fullStr | α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis |
| title_full_unstemmed | α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis |
| title_short | α2-fraction and haptoglobin as biomarkers for disease activity in oligo- and polyarticular juvenile idiopathic arthritis |
| title_sort | α2 fraction and haptoglobin as biomarkers for disease activity in oligo and polyarticular juvenile idiopathic arthritis |
| url | https://doi.org/10.1186/s12969-022-00721-7 |
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