Establishing reference intervals for von Willebrand factor multimers
Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fr...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2022-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582201115P.pdf |
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| author | Pikta Marika Vasse Marc Smock Kristi J. Moser Karen A. van Dievoet Marie-Astrid Lejniece Sandra Szanto Timea Bautista Hector Nouadje George Banys Valdas |
| author_facet | Pikta Marika Vasse Marc Smock Kristi J. Moser Karen A. van Dievoet Marie-Astrid Lejniece Sandra Szanto Timea Bautista Hector Nouadje George Banys Valdas |
| author_sort | Pikta Marika |
| collection | DOAJ |
| description | Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits. |
| first_indexed | 2024-04-13T16:29:03Z |
| format | Article |
| id | doaj.art-d3b9c5710a4d4857b15619f1703ad7e0 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-04-13T16:29:03Z |
| publishDate | 2022-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-d3b9c5710a4d4857b15619f1703ad7e02022-12-22T02:39:40ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662022-01-0141111512110.5937/jomb0-319411452-82582201115PEstablishing reference intervals for von Willebrand factor multimersPikta Marika0Vasse Marc1Smock Kristi J.2Moser Karen A.3van Dievoet Marie-Astrid4Lejniece Sandra5Szanto Timea6Bautista Hector7Nouadje George8Banys Valdas9North Estonia Medical Centre, Department of Laboratory Medicine, Tallinn, EstoniaFoch Hospital, Department of Biology & UMR INSERM 1176, Suresnes, FranceUniversity of Utah, School of Medicine, Department of Pathology, Salt Lake City, UT, USAUniversity of Utah, School of Medicine, Department of Pathology, Salt Lake City, UT, USACliniques Universitaires Saint-Luc, Laboratory Department, Bruxelles, BelgiumRiga Stradins University, Riga, LatviaHelsinki University Hospital Comprehensive Cancer Center and University of Helsinki, Department of Hematology, Coagulation Disorders Unit, Helsinki, FinlandSebia, Research and Developments Department, Lisses - Evry Cedex, FranceSebia, Research and Developments Department, Lisses - Evry Cedex, FranceVilnius University, Faculty of Medicine, Institute of Biomedical Sciences, Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Vilnius, LithuaniaBackground: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582201115P.pdfvon willebrand factorvon willebrand factor multimersquantitative analysisreference intervals |
| spellingShingle | Pikta Marika Vasse Marc Smock Kristi J. Moser Karen A. van Dievoet Marie-Astrid Lejniece Sandra Szanto Timea Bautista Hector Nouadje George Banys Valdas Establishing reference intervals for von Willebrand factor multimers Journal of Medical Biochemistry von willebrand factor von willebrand factor multimers quantitative analysis reference intervals |
| title | Establishing reference intervals for von Willebrand factor multimers |
| title_full | Establishing reference intervals for von Willebrand factor multimers |
| title_fullStr | Establishing reference intervals for von Willebrand factor multimers |
| title_full_unstemmed | Establishing reference intervals for von Willebrand factor multimers |
| title_short | Establishing reference intervals for von Willebrand factor multimers |
| title_sort | establishing reference intervals for von willebrand factor multimers |
| topic | von willebrand factor von willebrand factor multimers quantitative analysis reference intervals |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582201115P.pdf |
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