Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i>
<i>Scoparia dulcis</i> produces unique biologically active diterpenoids such as scopadulcic acid B (SDB). They are biosynthesized from geranylgeranyl diphosphate (GGPP) via <i>syn</i>-copalyl diphosphate (<i>syn</i>-CPP) and scopadulanol as an important key interm...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/1/69 |
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| author | Jung-Bum Lee Tomoya Ohmura Yoshimi Yamamura |
| author_facet | Jung-Bum Lee Tomoya Ohmura Yoshimi Yamamura |
| author_sort | Jung-Bum Lee |
| collection | DOAJ |
| description | <i>Scoparia dulcis</i> produces unique biologically active diterpenoids such as scopadulcic acid B (SDB). They are biosynthesized from geranylgeranyl diphosphate (GGPP) via <i>syn</i>-copalyl diphosphate (<i>syn</i>-CPP) and scopadulanol as an important key intermediate. In this paper, we functionally characterized three diterpene synthases, SdCPS2, SdKSL1 and SdKSL2, from <i>S. dulcis.</i> The SdCPS2 catalyzed a cyclization reaction from GGPP to <i>syn-</i>CPP, and SdKSL1 did from <i>syn-</i>CPP to scopadulan-13α-ol. On the other hand, SdKSL2 was found to incorporate a non-sense mutation at 682. Therefore, we mutated the nucleotide residue from A to G in SdKSL2 to produce SdKSL2mut, and it was able to recover the catalytic function from <i>syn</i>-CPP to <i>syn</i>-aphidicol-16-ene, the precursor to scopadulin. From our results, SdCPS2 and SdKSL1 might be important key players for SDB biosynthesis in <i>S. dulcis</i>. |
| first_indexed | 2024-03-09T03:27:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:27:48Z |
| publishDate | 2022-12-01 |
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| series | Plants |
| spelling | doaj.art-d3bc176e428d4d04a94074996ca5ac112023-12-03T15:00:22ZengMDPI AGPlants2223-77472022-12-011216910.3390/plants12010069Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i>Jung-Bum Lee0Tomoya Ohmura1Yoshimi Yamamura2Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanGraduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanGraduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan<i>Scoparia dulcis</i> produces unique biologically active diterpenoids such as scopadulcic acid B (SDB). They are biosynthesized from geranylgeranyl diphosphate (GGPP) via <i>syn</i>-copalyl diphosphate (<i>syn</i>-CPP) and scopadulanol as an important key intermediate. In this paper, we functionally characterized three diterpene synthases, SdCPS2, SdKSL1 and SdKSL2, from <i>S. dulcis.</i> The SdCPS2 catalyzed a cyclization reaction from GGPP to <i>syn-</i>CPP, and SdKSL1 did from <i>syn-</i>CPP to scopadulan-13α-ol. On the other hand, SdKSL2 was found to incorporate a non-sense mutation at 682. Therefore, we mutated the nucleotide residue from A to G in SdKSL2 to produce SdKSL2mut, and it was able to recover the catalytic function from <i>syn</i>-CPP to <i>syn</i>-aphidicol-16-ene, the precursor to scopadulin. From our results, SdCPS2 and SdKSL1 might be important key players for SDB biosynthesis in <i>S. dulcis</i>.https://www.mdpi.com/2223-7747/12/1/69diterpene synthase<i>Scoparia dulcis</i>cyclase |
| spellingShingle | Jung-Bum Lee Tomoya Ohmura Yoshimi Yamamura Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> Plants diterpene synthase <i>Scoparia dulcis</i> cyclase |
| title | Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> |
| title_full | Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> |
| title_fullStr | Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> |
| title_full_unstemmed | Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> |
| title_short | Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in <i>Scoparia dulcis</i> |
| title_sort | functional characterization of three diterpene synthases responsible for tetracyclic diterpene biosynthesis in i scoparia dulcis i |
| topic | diterpene synthase <i>Scoparia dulcis</i> cyclase |
| url | https://www.mdpi.com/2223-7747/12/1/69 |
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