Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation
The production of γ-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilizatio...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2018-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/290745 |
| _version_ | 1797207706455506944 |
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| author | Parvathy Sree Varma Salini Chandrasekharan Govindarajulu Venkateswaran Santhosh Rajendran Kiran Kumar Mallapureddy Ashok Pandey Binod Parameswaran |
| author_facet | Parvathy Sree Varma Salini Chandrasekharan Govindarajulu Venkateswaran Santhosh Rajendran Kiran Kumar Mallapureddy Ashok Pandey Binod Parameswaran |
| author_sort | Parvathy Sree Varma |
| collection | DOAJ |
| description | The production of γ-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that Cunninghamella elegans CFR C07 is a potent organism for the production of GLA under submerged conditions. |
| first_indexed | 2024-04-24T09:27:10Z |
| format | Article |
| id | doaj.art-d3bda6c90cb5455da1af3f1815488f37 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:27:10Z |
| publishDate | 2018-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-d3bda6c90cb5455da1af3f1815488f372024-04-15T14:42:13ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062018-01-015619610010.17113/ftb.56.01.18.5464Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged FermentationParvathy Sree Varma0Salini Chandrasekharan1Govindarajulu Venkateswaran2Santhosh Rajendran3Kiran Kumar Mallapureddy4Ashok Pandey5Binod Parameswaran6Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and TechnologyMicrobial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and TechnologyCSIR-Central Food Technological Research Institute, KajjihundiCSIR-Central Food Technological Research Institute, KajjihundiMicrobial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and TechnologyCSIR-Indian Institute of Toxicology ResearchMicrobial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and TechnologyThe production of γ-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that Cunninghamella elegans CFR C07 is a potent organism for the production of GLA under submerged conditions.https://hrcak.srce.hr/file/290745γ-linolenic acidsubmerged fermentationCunninghamella elegansfatty acid production |
| spellingShingle | Parvathy Sree Varma Salini Chandrasekharan Govindarajulu Venkateswaran Santhosh Rajendran Kiran Kumar Mallapureddy Ashok Pandey Binod Parameswaran Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation Food Technology and Biotechnology γ-linolenic acid submerged fermentation Cunninghamella elegans fatty acid production |
| title | Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation |
| title_full | Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation |
| title_fullStr | Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation |
| title_full_unstemmed | Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation |
| title_short | Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation |
| title_sort | optimization of process parameters for the production of γ linolenic acid by cunninghamella elegans cfr c07 in submerged fermentation |
| topic | γ-linolenic acid submerged fermentation Cunninghamella elegans fatty acid production |
| url | https://hrcak.srce.hr/file/290745 |
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