| Summary: | <b>Purpose:</b> To report the clinical and microbiological profile of endophthalmitis caused by <i> Acinetobacter calcoaceticus</i> . <b> Methods: </b> A retrospective study of case series of <i> Acinetobacter calcoaceticus</i> endophthalmitis. Outcome measures included ability to sterilise the eye, anatomical result (clear media and attached retina) and visual recovery (visual acuity > 6/60). <b> Results: </b> Of the 20 cases studied, 10 were cases of postoperative endophthalmitis, 3 were posttraumatic, 6 were endogenous and one was bleb-related endophthalmitis. Specific features of interest observed were relative chronicity of presentation and absence of any obvious predisposing factor in endogenous endophthalmitis cases. All cases could be sterilised except one, which needed evisceration. Cases with postoperative endophthalmitis had better anatomical outcome (7/10 with attached retina and clear media) and visual outcome (4/10 regained vision > 6/18). Higher smear positivity was seen in vitreous samples (72.2%) compared to aqueous samples (37.5%). Culture positivity was higher from the vitreous cavity compared to aqueous. The organism was sensitive to ciprofloxacin in a high percentage (88.9%) of cases. <b> Conclusions:</b> Visual recovery in <i> Acinetobacter calcoaceticus</i> endophthalmitis is modest. Ciprofloxacin is the antibiotic of choice
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