Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation

Seedlings of Trifolium pratense L. cultivars were mechanically inoculated with Czech isolates of Alfalfa mosaic virus (AMV), Clover yellow mosaic virus (ClYMV), Clover yellow vein virus (ClYVV), Red clover mottle virus (RCMV), White clover mosaic virus (WClMV), and a newly discovered member of the C...

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Main Authors: Jana Fránová, Hana Jakešová
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2014-09-01
Series:Plant Protection Science
Subjects:
Online Access:https://pps.agriculturejournals.cz/artkey/pps-201403-0001_susceptibility-of-ten-red-clover-trifolium-pratense-cultivars-to-six-viruses-after-artificial-inoculation.php
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author Jana Fránová
Hana Jakešová
author_facet Jana Fránová
Hana Jakešová
author_sort Jana Fránová
collection DOAJ
description Seedlings of Trifolium pratense L. cultivars were mechanically inoculated with Czech isolates of Alfalfa mosaic virus (AMV), Clover yellow mosaic virus (ClYMV), Clover yellow vein virus (ClYVV), Red clover mottle virus (RCMV), White clover mosaic virus (WClMV), and a newly discovered member of the Cytorhabdovirus genus. WClMV infected 75.4% of clover seedlings; cv. Rezista was the most susceptible (93.3%), while cv. Fresko was the least susceptible (58.3%). RCMV infected 59.6% of plants; the most susceptible was cv. Tempus (77.6%), the least susceptible cv. Sprint (38.3%). While WClMV infected a higher number of seedlings, RCMV revealed more severe symptoms on affected plants. On the basis of ELISA and RT-PCR results, no cultivar was susceptible to mechanical inoculation with ClYMV and cytorhabdovirus. Moreover, cvs Fresko and Sprint were not susceptible to ClYVV and AMV, respectively.
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spelling doaj.art-d3c14519382746f0bb5fb6aa0dde8ce42023-02-23T03:44:11ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412014-09-0150311311810.17221/71/2013-PPSpps-201403-0001Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculationJana Fránová0Hana Jakešová1Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre ASCR, České Budějovice, Czech RepublicIng. Hana Jakešová, CSc, Red Clover, Grass Breeding, Hladké Životice, Czech RepublicSeedlings of Trifolium pratense L. cultivars were mechanically inoculated with Czech isolates of Alfalfa mosaic virus (AMV), Clover yellow mosaic virus (ClYMV), Clover yellow vein virus (ClYVV), Red clover mottle virus (RCMV), White clover mosaic virus (WClMV), and a newly discovered member of the Cytorhabdovirus genus. WClMV infected 75.4% of clover seedlings; cv. Rezista was the most susceptible (93.3%), while cv. Fresko was the least susceptible (58.3%). RCMV infected 59.6% of plants; the most susceptible was cv. Tempus (77.6%), the least susceptible cv. Sprint (38.3%). While WClMV infected a higher number of seedlings, RCMV revealed more severe symptoms on affected plants. On the basis of ELISA and RT-PCR results, no cultivar was susceptible to mechanical inoculation with ClYMV and cytorhabdovirus. Moreover, cvs Fresko and Sprint were not susceptible to ClYVV and AMV, respectively.https://pps.agriculturejournals.cz/artkey/pps-201403-0001_susceptibility-of-ten-red-clover-trifolium-pratense-cultivars-to-six-viruses-after-artificial-inoculation.phpred clover mottle viruswhite clover mosaic virusdas-elisamechanical inoculation
spellingShingle Jana Fránová
Hana Jakešová
Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
Plant Protection Science
red clover mottle virus
white clover mosaic virus
das-elisa
mechanical inoculation
title Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
title_full Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
title_fullStr Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
title_full_unstemmed Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
title_short Susceptibility of ten red clover (Trifolium pratense) cultivars to six viruses after artificial inoculation
title_sort susceptibility of ten red clover trifolium pratense cultivars to six viruses after artificial inoculation
topic red clover mottle virus
white clover mosaic virus
das-elisa
mechanical inoculation
url https://pps.agriculturejournals.cz/artkey/pps-201403-0001_susceptibility-of-ten-red-clover-trifolium-pratense-cultivars-to-six-viruses-after-artificial-inoculation.php
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