AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex
The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 bind...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-16512-1 |
| _version_ | 1818851859115278336 |
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| author | Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft |
| author_facet | Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft |
| author_sort | Marscha Hirschi |
| collection | DOAJ |
| description | The anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA. |
| first_indexed | 2024-12-19T07:11:43Z |
| format | Article |
| id | doaj.art-d3c21875e57643ea9c2043b059ad21af |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T07:11:43Z |
| publishDate | 2020-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d3c21875e57643ea9c2043b059ad21af2022-12-21T20:31:10ZengNature PortfolioNature Communications2041-17232020-06-011111610.1038/s41467-020-16512-1AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complexMarscha Hirschi0Wang-Ting Lu1Andrew Santiago-Frangos2Royce Wilkinson3Sarah M. Golden4Alan R. Davidson5Gabriel C. Lander6Blake Wiedenheft7Department of Integrative Structural and Computational Biology, The Scripps Research InstituteDepartment of Molecular Genetics, and Department of Biochemistry, University of TorontoDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Microbiology and Immunology, Montana State UniversityDepartment of Molecular Genetics, and Department of Biochemistry, University of TorontoDepartment of Integrative Structural and Computational Biology, The Scripps Research InstituteDepartment of Microbiology and Immunology, Montana State UniversityThe anti-CRISPR protein IF9 (AcrIF9) specifically inhibits the type I-F CRISPR adaptive immune system. Here, the authors present the cryo-EM structure of AcrIF9 in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy) and a dsDNA bound Csy-AcrIF9 structure, and find that AcrIF9 binding to the Csy complex triggers non-sequence specific dsDNA binding to Csy-AcrIF9, which might sequester the complex from its target DNA.https://doi.org/10.1038/s41467-020-16512-1 |
| spellingShingle | Marscha Hirschi Wang-Ting Lu Andrew Santiago-Frangos Royce Wilkinson Sarah M. Golden Alan R. Davidson Gabriel C. Lander Blake Wiedenheft AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex Nature Communications |
| title | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
| title_full | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
| title_fullStr | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
| title_full_unstemmed | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
| title_short | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex |
| title_sort | acrif9 tethers non sequence specific dsdna to the crispr rna guided surveillance complex |
| url | https://doi.org/10.1038/s41467-020-16512-1 |
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