Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny
The effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg<sup>−1</sup> day<sup>−1</sup> glyphosate) for 5 weeks in 32-week-old roosters (<i&...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/2305-6304/9/12/318 |
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| author | Loïse Serra Anthony Estienne Guillaume Bourdon Christelle Ramé Claire Chevaleyre Philippe Didier Marine Chahnamian Souleiman El Balkhi Pascal Froment Joëlle Dupont |
| author_facet | Loïse Serra Anthony Estienne Guillaume Bourdon Christelle Ramé Claire Chevaleyre Philippe Didier Marine Chahnamian Souleiman El Balkhi Pascal Froment Joëlle Dupont |
| author_sort | Loïse Serra |
| collection | DOAJ |
| description | The effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg<sup>−1</sup> day<sup>−1</sup> glyphosate) for 5 weeks in 32-week-old roosters (<i>n</i> = 5 RU-exposed and <i>n</i> = 5 control (CT)). Although the concentrations of glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) increased in blood plasma and seminal fluid during exposure, no significant differences in testis weight and sperm concentrations were observed between RU and CT roosters. However, sperm motility was significantly reduced, associated with decreased calcium and ATP concentrations in RU spermatozoa. Plasma testosterone and oestradiol concentrations increased in RU roosters. These negative effects ceased 14 days after RU removal from the diet. Epigenetic analysis showed a global DNA hypomethylation in RU roosters. After artificial insemination of hens (<i>n</i> = 40) with sperm from CT or RU roosters, eggs were collected and artificially incubated. Embryo viability did not differ, but chicks from RU roosters (<i>n</i> = 118) had a higher food consumption, body weight and subcutaneous adipose tissue content. Chronic dietary RU exposure in roosters reduces sperm motility and increases plasma testosterone levels, growth performance, and fattening in offspring. |
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| last_indexed | 2024-03-10T03:59:24Z |
| publishDate | 2021-11-01 |
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| series | Toxics |
| spelling | doaj.art-d3c2d99be19f4acd992605f333c9aff82023-11-23T10:49:33ZengMDPI AGToxics2305-63042021-11-0191231810.3390/toxics9120318Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the ProgenyLoïse Serra0Anthony Estienne1Guillaume Bourdon2Christelle Ramé3Claire Chevaleyre4Philippe Didier5Marine Chahnamian6Souleiman El Balkhi7Pascal Froment8Joëlle Dupont9Centre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceInstitut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement—Unité Expérimentale du Pôle d’Expérimentation Avicole de Tours UEPEAT 1295, F-37380 Nouzilly, FranceInstitut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement—Unité Expérimentale du Pôle d’Expérimentation Avicole de Tours UEPEAT 1295, F-37380 Nouzilly, FranceService de Pharmacologie, Toxicologie et Pharmacovigilance, CHU, F-87042 Limoges, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceCentre National de la Recherche Scientifique, Institut Français du Cheval et de l’Equitation, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, Université de Tours, PRC, F-37380 Nouzilly, FranceThe effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg<sup>−1</sup> day<sup>−1</sup> glyphosate) for 5 weeks in 32-week-old roosters (<i>n</i> = 5 RU-exposed and <i>n</i> = 5 control (CT)). Although the concentrations of glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) increased in blood plasma and seminal fluid during exposure, no significant differences in testis weight and sperm concentrations were observed between RU and CT roosters. However, sperm motility was significantly reduced, associated with decreased calcium and ATP concentrations in RU spermatozoa. Plasma testosterone and oestradiol concentrations increased in RU roosters. These negative effects ceased 14 days after RU removal from the diet. Epigenetic analysis showed a global DNA hypomethylation in RU roosters. After artificial insemination of hens (<i>n</i> = 40) with sperm from CT or RU roosters, eggs were collected and artificially incubated. Embryo viability did not differ, but chicks from RU roosters (<i>n</i> = 118) had a higher food consumption, body weight and subcutaneous adipose tissue content. Chronic dietary RU exposure in roosters reduces sperm motility and increases plasma testosterone levels, growth performance, and fattening in offspring.https://www.mdpi.com/2305-6304/9/12/318glyphosateRoundupfertilityendocrine disruptorsmale chickenprogeny |
| spellingShingle | Loïse Serra Anthony Estienne Guillaume Bourdon Christelle Ramé Claire Chevaleyre Philippe Didier Marine Chahnamian Souleiman El Balkhi Pascal Froment Joëlle Dupont Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny Toxics glyphosate Roundup fertility endocrine disruptors male chicken progeny |
| title | Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny |
| title_full | Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny |
| title_fullStr | Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny |
| title_full_unstemmed | Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny |
| title_short | Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny |
| title_sort | chronic dietary exposure of roosters to a glyphosate based herbicide increases seminal plasma glyphosate and ampa concentrations alters sperm parameters and induces metabolic disorders in the progeny |
| topic | glyphosate Roundup fertility endocrine disruptors male chicken progeny |
| url | https://www.mdpi.com/2305-6304/9/12/318 |
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