AAV genome modification for efficient AAV production
The adeno-associated virus (AAV) is one of the most potent vectors in gene therapy. The experimental profile of this vector shows its efficiency and accepted safety, which explains its increased usage by scientists for the research and treatment of a wide range of diseases. These studies require usi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023022788 |
| _version_ | 1827957370397917184 |
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| author | Walaa Asaad Polina Volos Denis Maksimov Elena Khavina Andrei Deviatkin Olga Mityaeva Pavel Volchkov |
| author_facet | Walaa Asaad Polina Volos Denis Maksimov Elena Khavina Andrei Deviatkin Olga Mityaeva Pavel Volchkov |
| author_sort | Walaa Asaad |
| collection | DOAJ |
| description | The adeno-associated virus (AAV) is one of the most potent vectors in gene therapy. The experimental profile of this vector shows its efficiency and accepted safety, which explains its increased usage by scientists for the research and treatment of a wide range of diseases. These studies require using functional, pure, and high titers of vector particles. In fact, the current knowledge of AAV structure and genome helps improve the scalable production of AAV vectors. In this review, we summarize the latest studies on the optimization of scalable AAV production through modifying the AAV genome or biological processes inside the cell. |
| first_indexed | 2024-04-09T15:18:43Z |
| format | Article |
| id | doaj.art-d3c5cf6e88474a2886297eee5efe5186 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T15:18:43Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d3c5cf6e88474a2886297eee5efe51862023-04-29T14:55:10ZengElsevierHeliyon2405-84402023-04-0194e15071AAV genome modification for efficient AAV productionWalaa Asaad0Polina Volos1Denis Maksimov2Elena Khavina3Andrei Deviatkin4Olga Mityaeva5Pavel Volchkov6Corresponding author.; Genome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationGenome Engineering Laboratory, Moscow Institute of Physics and Technology, Dolgoprudniy, Russian FederationThe adeno-associated virus (AAV) is one of the most potent vectors in gene therapy. The experimental profile of this vector shows its efficiency and accepted safety, which explains its increased usage by scientists for the research and treatment of a wide range of diseases. These studies require using functional, pure, and high titers of vector particles. In fact, the current knowledge of AAV structure and genome helps improve the scalable production of AAV vectors. In this review, we summarize the latest studies on the optimization of scalable AAV production through modifying the AAV genome or biological processes inside the cell.http://www.sciencedirect.com/science/article/pii/S2405844023022788AAVAAV productionITRITRsAAPMAAP |
| spellingShingle | Walaa Asaad Polina Volos Denis Maksimov Elena Khavina Andrei Deviatkin Olga Mityaeva Pavel Volchkov AAV genome modification for efficient AAV production Heliyon AAV AAV production ITR ITRs AAP MAAP |
| title | AAV genome modification for efficient AAV production |
| title_full | AAV genome modification for efficient AAV production |
| title_fullStr | AAV genome modification for efficient AAV production |
| title_full_unstemmed | AAV genome modification for efficient AAV production |
| title_short | AAV genome modification for efficient AAV production |
| title_sort | aav genome modification for efficient aav production |
| topic | AAV AAV production ITR ITRs AAP MAAP |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023022788 |
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