Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue
Summary: Macrophages populate the embryo early in gestation, but their role in development is not well defined. In particular, specification and function of macrophages in intestinal development remain little explored. To study this event in the human developmental context, we derived and combined h...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723016285 |
| _version_ | 1797374188017680384 |
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| author | Andrew T. Song Renata H.M. Sindeaux Yuanyi Li Hicham Affia Tapan Agnihotri Severine Leclerc Patrick Piet van Vliet Mathieu Colas Jean-Victor Guimond Natalie Patey Lara Feulner Jean-Sebastien Joyal Elie Haddad Luis Barreiro Gregor Andelfinger |
| author_facet | Andrew T. Song Renata H.M. Sindeaux Yuanyi Li Hicham Affia Tapan Agnihotri Severine Leclerc Patrick Piet van Vliet Mathieu Colas Jean-Victor Guimond Natalie Patey Lara Feulner Jean-Sebastien Joyal Elie Haddad Luis Barreiro Gregor Andelfinger |
| author_sort | Andrew T. Song |
| collection | DOAJ |
| description | Summary: Macrophages populate the embryo early in gestation, but their role in development is not well defined. In particular, specification and function of macrophages in intestinal development remain little explored. To study this event in the human developmental context, we derived and combined human intestinal organoid and macrophages from pluripotent stem cells. Macrophages migrate into the organoid, proliferate, and occupy the emerging microanatomical niches of epithelial crypts and ganglia. They also acquire a transcriptomic profile similar to that of fetal intestinal macrophages and display tissue macrophage behaviors, such as recruitment to tissue injury. Using this model, we show that macrophages reduce glycolysis in mesenchymal cells and limit tissue growth without affecting tissue architecture, in contrast to the pro-growth effect of enteric neurons. In short, we engineered an intestinal tissue model populated with macrophages, and we suggest that resident macrophages contribute to the regulation of metabolism and growth of the developing intestine. |
| first_indexed | 2024-03-08T19:02:13Z |
| format | Article |
| id | doaj.art-d3c7df78f95347ef9599f98c5a997049 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-08T19:02:13Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d3c7df78f95347ef9599f98c5a9970492023-12-28T05:16:31ZengElsevierCell Reports2211-12472024-01-01431113616Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissueAndrew T. Song0Renata H.M. Sindeaux1Yuanyi Li2Hicham Affia3Tapan Agnihotri4Severine Leclerc5Patrick Piet van Vliet6Mathieu Colas7Jean-Victor Guimond8Natalie Patey9Lara Feulner10Jean-Sebastien Joyal11Elie Haddad12Luis Barreiro13Gregor Andelfinger14Centre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada; Corresponding authorCentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Meakins Christie Laboratories, Department of Medicine, Department of Microbiology and Immunology, Department of Pathology Research Institute of McGill University Health Centre, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCLSC des Faubourgs, CIUSSS du Centre-Sud-de-l’Ile-de-Montréal, Montréal, QC, CanadaDepartment of Pathology, CHU Sainte-Justine, Université de Montréal, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Département de Pédiatrie, Université de Montréal, Montréal, QC, Canada; Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Département de Pédiatrie, Université de Montréal, Montréal, QC, CanadaCentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Genetics Genomics and Systems Biology, University of Chicago, Chicago, IL, USACentre de Recherche, CHU Sainte-Justine, Montréal, QC, Canada; Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada; Département de Pédiatrie, Université de Montréal, Montréal, QC, Canada; Corresponding authorSummary: Macrophages populate the embryo early in gestation, but their role in development is not well defined. In particular, specification and function of macrophages in intestinal development remain little explored. To study this event in the human developmental context, we derived and combined human intestinal organoid and macrophages from pluripotent stem cells. Macrophages migrate into the organoid, proliferate, and occupy the emerging microanatomical niches of epithelial crypts and ganglia. They also acquire a transcriptomic profile similar to that of fetal intestinal macrophages and display tissue macrophage behaviors, such as recruitment to tissue injury. Using this model, we show that macrophages reduce glycolysis in mesenchymal cells and limit tissue growth without affecting tissue architecture, in contrast to the pro-growth effect of enteric neurons. In short, we engineered an intestinal tissue model populated with macrophages, and we suggest that resident macrophages contribute to the regulation of metabolism and growth of the developing intestine.http://www.sciencedirect.com/science/article/pii/S2211124723016285CP: Stem cell research |
| spellingShingle | Andrew T. Song Renata H.M. Sindeaux Yuanyi Li Hicham Affia Tapan Agnihotri Severine Leclerc Patrick Piet van Vliet Mathieu Colas Jean-Victor Guimond Natalie Patey Lara Feulner Jean-Sebastien Joyal Elie Haddad Luis Barreiro Gregor Andelfinger Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue Cell Reports CP: Stem cell research |
| title | Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue |
| title_full | Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue |
| title_fullStr | Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue |
| title_full_unstemmed | Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue |
| title_short | Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue |
| title_sort | developmental role of macrophages modeled in human pluripotent stem cell derived intestinal tissue |
| topic | CP: Stem cell research |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723016285 |
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