Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1
Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus and is commonly found in cereals, oils and foodstuffs. In order to understand the toxic effects of AFB1 exposure on Porcine alveolar macrophages (3D4/2 cell), the 3D4/2 cells were exposed...
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MDPI AG
2022-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/6/373 |
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| author | Huhe Chao Haohai Ma Jiadong Sun Shuai Yuan Peiyu Dong Aihong Zhao Lan Li Wei Shen Xifeng Zhang |
| author_facet | Huhe Chao Haohai Ma Jiadong Sun Shuai Yuan Peiyu Dong Aihong Zhao Lan Li Wei Shen Xifeng Zhang |
| author_sort | Huhe Chao |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus and is commonly found in cereals, oils and foodstuffs. In order to understand the toxic effects of AFB1 exposure on Porcine alveolar macrophages (3D4/2 cell), the 3D4/2 cells were exposed to 40 μg/mL AFB1 for 24 h in vitro, and several methods were used for analysis. Edu and TUNEL analysis showed that the proliferation of 3D4/2 cells was significantly inhibited and the apoptosis of 3D4/2 cells was significantly induced after AFB1 exposure compared with that of the control group. Whole-transcriptome analysis was performed to reveal the non-coding RNA alteration in 3D4/2 cells after AFB1 exposure. It was found that the expression of cell-cycle-related and apoptosis-related genes was altered after AFB1 exposure, and lncRNAs and miRNAs were also significantly different among the experimental groups. In particular, AFB1 exposure affected the expression of lncRNAs associated with cellular senescence signaling pathways, such as MSTRG.24315 and MSTRG.80767, as well as related genes, Cxcl8 and Gadd45g. In addition, AFB1 exposure affected the expression of miRNAs associated with immune-related genes, such as miR-181a, miR-331-3p and miR-342, as well as immune-related genes Nfkb1 and Rras2. Moreover, the regulation networks between mRNA-miRNAs and mRNA-lncRNAs were confirmed by the results of RT-qPCR and immunofluorescence. In conclusion, our results here demonstrate that AFB1 exposure impaired proliferation of 3D4/2 cells via the non-coding RNA-mediated pathway. |
| first_indexed | 2024-03-09T22:19:38Z |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T22:19:38Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d3c9d335ed1c43a2a173e6a7cb9be28f2023-11-23T19:16:50ZengMDPI AGToxins2072-66512022-05-0114637310.3390/toxins14060373Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1Huhe Chao0Haohai Ma1Jiadong Sun2Shuai Yuan3Peiyu Dong4Aihong Zhao5Lan Li6Wei Shen7Xifeng Zhang8College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaKey Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Medicine, Henan Polytechnic University, Jiaozuo 454000, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaQingdao Academy of Agricultural Science, Qingdao 266100, ChinaKey Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaKey Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaAflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus and is commonly found in cereals, oils and foodstuffs. In order to understand the toxic effects of AFB1 exposure on Porcine alveolar macrophages (3D4/2 cell), the 3D4/2 cells were exposed to 40 μg/mL AFB1 for 24 h in vitro, and several methods were used for analysis. Edu and TUNEL analysis showed that the proliferation of 3D4/2 cells was significantly inhibited and the apoptosis of 3D4/2 cells was significantly induced after AFB1 exposure compared with that of the control group. Whole-transcriptome analysis was performed to reveal the non-coding RNA alteration in 3D4/2 cells after AFB1 exposure. It was found that the expression of cell-cycle-related and apoptosis-related genes was altered after AFB1 exposure, and lncRNAs and miRNAs were also significantly different among the experimental groups. In particular, AFB1 exposure affected the expression of lncRNAs associated with cellular senescence signaling pathways, such as MSTRG.24315 and MSTRG.80767, as well as related genes, Cxcl8 and Gadd45g. In addition, AFB1 exposure affected the expression of miRNAs associated with immune-related genes, such as miR-181a, miR-331-3p and miR-342, as well as immune-related genes Nfkb1 and Rras2. Moreover, the regulation networks between mRNA-miRNAs and mRNA-lncRNAs were confirmed by the results of RT-qPCR and immunofluorescence. In conclusion, our results here demonstrate that AFB1 exposure impaired proliferation of 3D4/2 cells via the non-coding RNA-mediated pathway.https://www.mdpi.com/2072-6651/14/6/373aflatoxin B1porcineporcine alveolar macrophagesapoptosiscell cycle |
| spellingShingle | Huhe Chao Haohai Ma Jiadong Sun Shuai Yuan Peiyu Dong Aihong Zhao Lan Li Wei Shen Xifeng Zhang Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 Toxins aflatoxin B1 porcine porcine alveolar macrophages apoptosis cell cycle |
| title | Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 |
| title_full | Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 |
| title_fullStr | Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 |
| title_full_unstemmed | Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 |
| title_short | Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1 |
| title_sort | whole transcriptome analysis of non coding rna alteration in porcine alveolar macrophage exposed to aflatoxin b1 |
| topic | aflatoxin B1 porcine porcine alveolar macrophages apoptosis cell cycle |
| url | https://www.mdpi.com/2072-6651/14/6/373 |
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