Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster
Abstract Axonal terminals of the small ventral lateral neurons (sLNvs), the circadian clock neurons of Drosophila, show daily changes in their arborization complexity, with many branches in the morning and their shrinkage during the night. This complex phenomenon is precisely regulated by several me...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-56649-3 |
| _version_ | 1797259312416948224 |
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| author | Kornel Szypulski Aleksandra Tyszka Elzbieta Pyza Milena Damulewicz |
| author_facet | Kornel Szypulski Aleksandra Tyszka Elzbieta Pyza Milena Damulewicz |
| author_sort | Kornel Szypulski |
| collection | DOAJ |
| description | Abstract Axonal terminals of the small ventral lateral neurons (sLNvs), the circadian clock neurons of Drosophila, show daily changes in their arborization complexity, with many branches in the morning and their shrinkage during the night. This complex phenomenon is precisely regulated by several mechanisms. In the present study we describe that one of them is autophagy, a self-degradative process, also involved in changes of cell membrane size and shape. Our results showed that autophagosome formation and processing in PDF-expressing neurons (both sLNv and lLNv) are rhythmic and they have different patterns in the cell bodies and terminals. These rhythmic changes in the autophagy activity seem to be important for neuronal plasticity. We found that autophagosome cargos are different during the day and night, and more proteins involved in membrane remodeling are present in autophagosomes in the morning. In addition, we described for the first time that Atg8-positive vesicles are also present outside the sLNv terminals, which suggests that secretory autophagy might be involved in regulating the clock signaling network. Our data indicate that rhythmic autophagy in clock neurons affect the pacemaker function, through remodeling of terminal membrane and secretion of specific proteins from sLNvs. |
| first_indexed | 2024-04-24T23:07:26Z |
| format | Article |
| id | doaj.art-d3cf4ea58b7744b0b32f2d526f7e9405 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:07:26Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d3cf4ea58b7744b0b32f2d526f7e94052024-03-17T12:26:32ZengNature PortfolioScientific Reports2045-23222024-03-0114111510.1038/s41598-024-56649-3Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogasterKornel Szypulski0Aleksandra Tyszka1Elzbieta Pyza2Milena Damulewicz3Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian UniversityDepartment of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian UniversityDepartment of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian UniversityDepartment of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian UniversityAbstract Axonal terminals of the small ventral lateral neurons (sLNvs), the circadian clock neurons of Drosophila, show daily changes in their arborization complexity, with many branches in the morning and their shrinkage during the night. This complex phenomenon is precisely regulated by several mechanisms. In the present study we describe that one of them is autophagy, a self-degradative process, also involved in changes of cell membrane size and shape. Our results showed that autophagosome formation and processing in PDF-expressing neurons (both sLNv and lLNv) are rhythmic and they have different patterns in the cell bodies and terminals. These rhythmic changes in the autophagy activity seem to be important for neuronal plasticity. We found that autophagosome cargos are different during the day and night, and more proteins involved in membrane remodeling are present in autophagosomes in the morning. In addition, we described for the first time that Atg8-positive vesicles are also present outside the sLNv terminals, which suggests that secretory autophagy might be involved in regulating the clock signaling network. Our data indicate that rhythmic autophagy in clock neurons affect the pacemaker function, through remodeling of terminal membrane and secretion of specific proteins from sLNvs.https://doi.org/10.1038/s41598-024-56649-3Circadian clockSleepNeuroplasticityThe fruit fly |
| spellingShingle | Kornel Szypulski Aleksandra Tyszka Elzbieta Pyza Milena Damulewicz Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster Scientific Reports Circadian clock Sleep Neuroplasticity The fruit fly |
| title | Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster |
| title_full | Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster |
| title_fullStr | Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster |
| title_full_unstemmed | Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster |
| title_short | Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster |
| title_sort | autophagy as a new player in the regulation of clock neurons physiology of drosophila melanogaster |
| topic | Circadian clock Sleep Neuroplasticity The fruit fly |
| url | https://doi.org/10.1038/s41598-024-56649-3 |
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