Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway
Abstract To find out the role of hsa‐miR‐570‐3p targeting CD274 in triple negative breast cancer (TNBC) via PI3K/AKT/mTOR signaling pathway. Hsa‐miR‐570‐3p and CD274 expressions in 175 TNBC patients were detected by qRT‐PCR and immunohistochemistry respectively. The human TNBC cell lines (MDA‐MB‐468...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-08-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12212 |
| _version_ | 1797392894852595712 |
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| author | Li‐Li Wang Wei‐Wei Huang Jing Huang Rong‐Fang Huang Na‐Ni Li Yi Hong Mu‐Lan Chen Fan Wu Jian Liu |
| author_facet | Li‐Li Wang Wei‐Wei Huang Jing Huang Rong‐Fang Huang Na‐Ni Li Yi Hong Mu‐Lan Chen Fan Wu Jian Liu |
| author_sort | Li‐Li Wang |
| collection | DOAJ |
| description | Abstract To find out the role of hsa‐miR‐570‐3p targeting CD274 in triple negative breast cancer (TNBC) via PI3K/AKT/mTOR signaling pathway. Hsa‐miR‐570‐3p and CD274 expressions in 175 TNBC patients were detected by qRT‐PCR and immunohistochemistry respectively. The human TNBC cell lines (MDA‐MB‐468 and MDA‐MB‐231) were used to verify the targeting relationship between hsa‐miR‐570‐3p and CD274 via dual‐luciferase reporter gene assay. Then, MDA‐MB‐468 and MDA‐MB‐231 cells were divided into Blank, miR‐NC, miR‐570‐3p mimics, NC siRNA, CD274 siRNA, and miR‐570‐3p inhibitors + CD274 siRNA groups. Next, the biological activities of cells were detected by MTT, Cell‐Light EdU, Annexin‐V‐FITC/PI, wound healing and Transwell invasion assays. Western blotting was conducted to detect protein expressions.MiR‐570‐3p expression was lower in tumor tissues than that in adjacent normal tissues, which was more obvious in CD274‐positive TNBC patients, which targeted CD274 in TNBC cell lines. MiR‐570‐3p inhibited cell proliferation, invasion and migration, but induced cell apoptosis accompanying the upregulation of apoptotic proteins and downregulation of anti‐apoptotic protein. CD274 siRNA had the similar results of miR‐570‐3p mimics, which could be reversed by miR‐570‐3p inhibitors. Besides, both miR‐570‐3p mimics and CD274 siRNA blocked PI3K/AKT/mTOR signaling pathway in TNBC cell lines. Hsa‐miR‐570‐3p was downregulated and CD274 was upregulated in TNBC patients. Besides, hsa‐miR‐570‐3p targeted CD274 to inhibit cell proliferation, invasion, migration, and induce cell apoptosis, which may be related to the suppression of PI3K/AKT/mTOR pathway. |
| first_indexed | 2024-03-08T23:55:08Z |
| format | Article |
| id | doaj.art-d3cf7b8a0ed24944ab44bb748f956a7f |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-03-08T23:55:08Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-d3cf7b8a0ed24944ab44bb748f956a7f2023-12-13T07:10:58ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502020-08-0136858159110.1002/kjm2.12212Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathwayLi‐Li Wang0Wei‐Wei Huang1Jing Huang2Rong‐Fang Huang3Na‐Ni Li4Yi Hong5Mu‐Lan Chen6Fan Wu7Jian Liu8Department of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Pharmacy Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Pathology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaDepartment of Medical Oncology Fujian Cancer Hospital & Fujian Medical University Cancer Hospital Fuzhou Fujian Province ChinaAbstract To find out the role of hsa‐miR‐570‐3p targeting CD274 in triple negative breast cancer (TNBC) via PI3K/AKT/mTOR signaling pathway. Hsa‐miR‐570‐3p and CD274 expressions in 175 TNBC patients were detected by qRT‐PCR and immunohistochemistry respectively. The human TNBC cell lines (MDA‐MB‐468 and MDA‐MB‐231) were used to verify the targeting relationship between hsa‐miR‐570‐3p and CD274 via dual‐luciferase reporter gene assay. Then, MDA‐MB‐468 and MDA‐MB‐231 cells were divided into Blank, miR‐NC, miR‐570‐3p mimics, NC siRNA, CD274 siRNA, and miR‐570‐3p inhibitors + CD274 siRNA groups. Next, the biological activities of cells were detected by MTT, Cell‐Light EdU, Annexin‐V‐FITC/PI, wound healing and Transwell invasion assays. Western blotting was conducted to detect protein expressions.MiR‐570‐3p expression was lower in tumor tissues than that in adjacent normal tissues, which was more obvious in CD274‐positive TNBC patients, which targeted CD274 in TNBC cell lines. MiR‐570‐3p inhibited cell proliferation, invasion and migration, but induced cell apoptosis accompanying the upregulation of apoptotic proteins and downregulation of anti‐apoptotic protein. CD274 siRNA had the similar results of miR‐570‐3p mimics, which could be reversed by miR‐570‐3p inhibitors. Besides, both miR‐570‐3p mimics and CD274 siRNA blocked PI3K/AKT/mTOR signaling pathway in TNBC cell lines. Hsa‐miR‐570‐3p was downregulated and CD274 was upregulated in TNBC patients. Besides, hsa‐miR‐570‐3p targeted CD274 to inhibit cell proliferation, invasion, migration, and induce cell apoptosis, which may be related to the suppression of PI3K/AKT/mTOR pathway.https://doi.org/10.1002/kjm2.12212CD274hsa‐miR‐570‐3pPI3K/AKT/mTORtriple negative breast cancer |
| spellingShingle | Li‐Li Wang Wei‐Wei Huang Jing Huang Rong‐Fang Huang Na‐Ni Li Yi Hong Mu‐Lan Chen Fan Wu Jian Liu Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway Kaohsiung Journal of Medical Sciences CD274 hsa‐miR‐570‐3p PI3K/AKT/mTOR triple negative breast cancer |
| title | Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway |
| title_full | Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway |
| title_fullStr | Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway |
| title_full_unstemmed | Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway |
| title_short | Retracted: Protective effect of hsa‐miR‐570‐3p targeting CD274 on triple negative breast cancer by blocking PI3K/AKT/mTOR signaling pathway |
| title_sort | retracted protective effect of hsa mir 570 3p targeting cd274 on triple negative breast cancer by blocking pi3k akt mtor signaling pathway |
| topic | CD274 hsa‐miR‐570‐3p PI3K/AKT/mTOR triple negative breast cancer |
| url | https://doi.org/10.1002/kjm2.12212 |
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