UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription
Summary: More than half the world’s population is infected with human cytomegalovirus (HCMV), causing congenital birth defects and impacting the immuno-compromised. Many of the >170 HCMV genes remain uncharacterized, and this gap in knowledge limits the development of novel antivirals. In this st...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222014407 |
| _version_ | 1811206333118021632 |
|---|---|
| author | Declan L. Turner Svenja Fritzlar Sara Sadeghipour Adele A. Barugahare Brendan E. Russ Stephen J. Turner Rommel A. Mathias |
| author_facet | Declan L. Turner Svenja Fritzlar Sara Sadeghipour Adele A. Barugahare Brendan E. Russ Stephen J. Turner Rommel A. Mathias |
| author_sort | Declan L. Turner |
| collection | DOAJ |
| description | Summary: More than half the world’s population is infected with human cytomegalovirus (HCMV), causing congenital birth defects and impacting the immuno-compromised. Many of the >170 HCMV genes remain uncharacterized, and this gap in knowledge limits the development of novel antivirals. In this study, we investigated the essential viral protein UL49 and found it displayed leaky late expression kinetics, and localized to nuclear replication compartments. Cells infected with mutant UL49 virus were unable to produce infectious virions and phenocopied other beta-gamma viral pre-initiation complex (vPIC) subunit (UL79, UL87, UL91, UL92, and UL95) mutant infections. RNA-seq analysis of vPIC mutant infections revealed a consistent diminution of genes encoding capsid subunits, including TRX2/UL85 and MCP/UL86, envelope glycoproteins gM, gL and gO, and egress-associated tegument proteins UL99 and UL103. Therefore, as a member of the vPIC, UL49 serves as a fundamental HCMV effector that governs viral gene transcription required to complete the replication cycle. |
| first_indexed | 2024-04-12T03:45:35Z |
| format | Article |
| id | doaj.art-d3d3183e099843a985101a7dec9804bf |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T03:45:35Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d3d3183e099843a985101a7dec9804bf2022-12-22T03:49:09ZengElsevieriScience2589-00422022-10-012510105168UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcriptionDeclan L. Turner0Svenja Fritzlar1Sara Sadeghipour2Adele A. Barugahare3Brendan E. Russ4Stephen J. Turner5Rommel A. Mathias6Infection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, Australia; Monash Bioinformatics Platform, Monash University, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, AustraliaInfection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, Australia; Department of Biochemistry and Molecular Biology, Monash University, 23 Innovation Walk, Clayton, VIC 3800, Australia; Corresponding authorSummary: More than half the world’s population is infected with human cytomegalovirus (HCMV), causing congenital birth defects and impacting the immuno-compromised. Many of the >170 HCMV genes remain uncharacterized, and this gap in knowledge limits the development of novel antivirals. In this study, we investigated the essential viral protein UL49 and found it displayed leaky late expression kinetics, and localized to nuclear replication compartments. Cells infected with mutant UL49 virus were unable to produce infectious virions and phenocopied other beta-gamma viral pre-initiation complex (vPIC) subunit (UL79, UL87, UL91, UL92, and UL95) mutant infections. RNA-seq analysis of vPIC mutant infections revealed a consistent diminution of genes encoding capsid subunits, including TRX2/UL85 and MCP/UL86, envelope glycoproteins gM, gL and gO, and egress-associated tegument proteins UL99 and UL103. Therefore, as a member of the vPIC, UL49 serves as a fundamental HCMV effector that governs viral gene transcription required to complete the replication cycle.http://www.sciencedirect.com/science/article/pii/S2589004222014407Molecular biologyvirologytranscriptomics |
| spellingShingle | Declan L. Turner Svenja Fritzlar Sara Sadeghipour Adele A. Barugahare Brendan E. Russ Stephen J. Turner Rommel A. Mathias UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription iScience Molecular biology virology transcriptomics |
| title | UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription |
| title_full | UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription |
| title_fullStr | UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription |
| title_full_unstemmed | UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription |
| title_short | UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription |
| title_sort | ul49 is an essential subunit of the viral pre initiation complex that regulates human cytomegalovirus gene transcription |
| topic | Molecular biology virology transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222014407 |
| work_keys_str_mv | AT declanlturner ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT svenjafritzlar ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT sarasadeghipour ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT adeleabarugahare ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT brendaneruss ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT stephenjturner ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription AT rommelamathias ul49isanessentialsubunitoftheviralpreinitiationcomplexthatregulateshumancytomegalovirusgenetranscription |