Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken
Abstract Background Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) is a zoonotic pathogen responsible for severe intestinal pathology in young chickens. Natural resistance-associated macrophage protein (NRAMP) family has been shown to be associated with resistance to intracellular...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1510-4 |
| _version_ | 1818932650997448704 |
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| author | Mashooq Ahmad Dar Raashid Ahmed Uneeb Urwat Syed Mudasir Ahmad Pervaiz Ahmad Dar Zahid Amin Kushoo Tanveer Ali Dar Peerzada Tajamul Mumtaz Shakil Ahmad Bhat Umar Amin Nadeem Shabir Hina Fayaz Bhat Riaz Ahmad Shah Nazir Ahmad Ganai Mohammad Heidari |
| author_facet | Mashooq Ahmad Dar Raashid Ahmed Uneeb Urwat Syed Mudasir Ahmad Pervaiz Ahmad Dar Zahid Amin Kushoo Tanveer Ali Dar Peerzada Tajamul Mumtaz Shakil Ahmad Bhat Umar Amin Nadeem Shabir Hina Fayaz Bhat Riaz Ahmad Shah Nazir Ahmad Ganai Mohammad Heidari |
| author_sort | Mashooq Ahmad Dar |
| collection | DOAJ |
| description | Abstract Background Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) is a zoonotic pathogen responsible for severe intestinal pathology in young chickens. Natural resistance-associated macrophage protein (NRAMP) family has been shown to be associated with resistance to intracellular pathogens, including Salmonella Typhimurium. The role of NRAMP proteins in macrophage defence against microbial infection has been ascribed to changes in the metal-ion concentrations inside the bacteria-containing phagosomes. The present study was conducted to investigate tissue-specific (liver, spleen and caecum) expression kinetics of NRAMP gene family (NRAMP1 and NRAMP2) in broilers from day 0 to day 15 after Salmonella Typhimurium challenge concomitant to clinical, blood biochemical and immunological parameters survey. Results Clinical symptoms appeared 4 days post-infection (dpi) in infected birds. Symptoms like progressive weakness, anorexia, diarrhoea and lowering of the head were seen in infected birds one-week post-infection. On postmortem examination, liver showed congestion, haemorrhage and necrotic foci on the surface, while as the spleen, lungs and intestines revealed congestion and haemorrhages. Histopathological alterations were principally found in liver comprising of necrosis, reticular endothelial hyperplasia along with mononuclear cell and heterophilic infiltration. Red Blood Cell (RBC) count, Haemoglobin (Hb) and Packed Cell Volume (PCV) decreased significantly (P < 0.05) in blood while heterophil counts increased up to 7 days post-infection. Serum glucose, aspartate transaminase (AST) and alanine transaminase (ALT) enzymes concentrations increased significantly throughout the study. A gradual increase of specific humoral IgG response confirmed Salmonella infection. Meanwhile, expression of NRAMP1 and NRAMP2 genes was differentially regulated after infection in tissues such as liver, spleen and caecum known to be the target of Salmonella Typhimurium replication in the chicken. Conclusion Thus the specific roles of NRAMP1 and NRAMP2 genes in Salmonella Typhimurium induced disease may be supposed from their differential expression according to tissues and timing after per os infection. However, these roles remain to be analyzed related to the severity of the disease which can be estimated by blood biochemistry and immunological parameters. |
| first_indexed | 2024-12-20T04:35:52Z |
| format | Article |
| id | doaj.art-d3d34af453904f06975d0970a118f616 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T04:35:52Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-d3d34af453904f06975d0970a118f6162022-12-21T19:53:16ZengBMCBMC Veterinary Research1746-61482018-06-0114111110.1186/s12917-018-1510-4Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chickenMashooq Ahmad Dar0Raashid Ahmed1Uneeb Urwat2Syed Mudasir Ahmad3Pervaiz Ahmad Dar4Zahid Amin Kushoo5Tanveer Ali Dar6Peerzada Tajamul Mumtaz7Shakil Ahmad Bhat8Umar Amin9Nadeem Shabir10Hina Fayaz Bhat11Riaz Ahmad Shah12Nazir Ahmad Ganai13Mohammad Heidari14Division of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Veterinary Microbiology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, SKUAST-KDivision of Veterinary Microbiology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, SKUAST-KDepatment of Biochemistry, University of KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, SKUAST-KDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Sher-e- Kashmir University of Agricultural Sciences and Technology – KashmirDivision of Animal Breeding and Genetics, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, SKUAST-KUSDA, Agricultural Research Service, Avian Disease and Oncology LaboratoryAbstract Background Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) is a zoonotic pathogen responsible for severe intestinal pathology in young chickens. Natural resistance-associated macrophage protein (NRAMP) family has been shown to be associated with resistance to intracellular pathogens, including Salmonella Typhimurium. The role of NRAMP proteins in macrophage defence against microbial infection has been ascribed to changes in the metal-ion concentrations inside the bacteria-containing phagosomes. The present study was conducted to investigate tissue-specific (liver, spleen and caecum) expression kinetics of NRAMP gene family (NRAMP1 and NRAMP2) in broilers from day 0 to day 15 after Salmonella Typhimurium challenge concomitant to clinical, blood biochemical and immunological parameters survey. Results Clinical symptoms appeared 4 days post-infection (dpi) in infected birds. Symptoms like progressive weakness, anorexia, diarrhoea and lowering of the head were seen in infected birds one-week post-infection. On postmortem examination, liver showed congestion, haemorrhage and necrotic foci on the surface, while as the spleen, lungs and intestines revealed congestion and haemorrhages. Histopathological alterations were principally found in liver comprising of necrosis, reticular endothelial hyperplasia along with mononuclear cell and heterophilic infiltration. Red Blood Cell (RBC) count, Haemoglobin (Hb) and Packed Cell Volume (PCV) decreased significantly (P < 0.05) in blood while heterophil counts increased up to 7 days post-infection. Serum glucose, aspartate transaminase (AST) and alanine transaminase (ALT) enzymes concentrations increased significantly throughout the study. A gradual increase of specific humoral IgG response confirmed Salmonella infection. Meanwhile, expression of NRAMP1 and NRAMP2 genes was differentially regulated after infection in tissues such as liver, spleen and caecum known to be the target of Salmonella Typhimurium replication in the chicken. Conclusion Thus the specific roles of NRAMP1 and NRAMP2 genes in Salmonella Typhimurium induced disease may be supposed from their differential expression according to tissues and timing after per os infection. However, these roles remain to be analyzed related to the severity of the disease which can be estimated by blood biochemistry and immunological parameters.http://link.springer.com/article/10.1186/s12917-018-1510-4Salmonella TyphimuriumPoultryHistopathologyBiochemistryReal time expressionNRAMP |
| spellingShingle | Mashooq Ahmad Dar Raashid Ahmed Uneeb Urwat Syed Mudasir Ahmad Pervaiz Ahmad Dar Zahid Amin Kushoo Tanveer Ali Dar Peerzada Tajamul Mumtaz Shakil Ahmad Bhat Umar Amin Nadeem Shabir Hina Fayaz Bhat Riaz Ahmad Shah Nazir Ahmad Ganai Mohammad Heidari Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken BMC Veterinary Research Salmonella Typhimurium Poultry Histopathology Biochemistry Real time expression NRAMP |
| title | Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken |
| title_full | Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken |
| title_fullStr | Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken |
| title_full_unstemmed | Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken |
| title_short | Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken |
| title_sort | expression kinetics of natural resistance associated macrophage protein nramp genes in salmonella typhimurium infected chicken |
| topic | Salmonella Typhimurium Poultry Histopathology Biochemistry Real time expression NRAMP |
| url | http://link.springer.com/article/10.1186/s12917-018-1510-4 |
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