Kinetic Analysis by Affinity Chromatography
Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that may be used to examine the kinetics of biologica...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2019.00673/full |
| _version_ | 1818942804762558464 |
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| author | Sazia Iftekhar Susan T. Ovbude David S. Hage |
| author_facet | Sazia Iftekhar Susan T. Ovbude David S. Hage |
| author_sort | Sazia Iftekhar |
| collection | DOAJ |
| description | Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that may be used to examine the kinetics of biological reactions. These methods include band-broadening measurements, techniques for peak fitting, split-peak analysis, peak decay studies, and ultrafast affinity extraction. The general principles and theory of each method, as applied to the determination of rate constants, will be discussed. The applications of each approach, along with its advantages and limitations, will also be considered. |
| first_indexed | 2024-12-20T07:17:15Z |
| format | Article |
| id | doaj.art-d3d4082e02b54e04b0e6ae891590476f |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-20T07:17:15Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-d3d4082e02b54e04b0e6ae891590476f2022-12-21T19:48:46ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-10-01710.3389/fchem.2019.00673483878Kinetic Analysis by Affinity ChromatographySazia IftekharSusan T. OvbudeDavid S. HageImportant information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that may be used to examine the kinetics of biological reactions. These methods include band-broadening measurements, techniques for peak fitting, split-peak analysis, peak decay studies, and ultrafast affinity extraction. The general principles and theory of each method, as applied to the determination of rate constants, will be discussed. The applications of each approach, along with its advantages and limitations, will also be considered.https://www.frontiersin.org/article/10.3389/fchem.2019.00673/fullaffinity chromatographybiological interactionskineticspeak profilingpeak decay methodplate height method |
| spellingShingle | Sazia Iftekhar Susan T. Ovbude David S. Hage Kinetic Analysis by Affinity Chromatography Frontiers in Chemistry affinity chromatography biological interactions kinetics peak profiling peak decay method plate height method |
| title | Kinetic Analysis by Affinity Chromatography |
| title_full | Kinetic Analysis by Affinity Chromatography |
| title_fullStr | Kinetic Analysis by Affinity Chromatography |
| title_full_unstemmed | Kinetic Analysis by Affinity Chromatography |
| title_short | Kinetic Analysis by Affinity Chromatography |
| title_sort | kinetic analysis by affinity chromatography |
| topic | affinity chromatography biological interactions kinetics peak profiling peak decay method plate height method |
| url | https://www.frontiersin.org/article/10.3389/fchem.2019.00673/full |
| work_keys_str_mv | AT saziaiftekhar kineticanalysisbyaffinitychromatography AT susantovbude kineticanalysisbyaffinitychromatography AT davidshage kineticanalysisbyaffinitychromatography |