Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration
Large bone defects have been a major healthcare concern. These defects are difficult to heal because of the chronic inflammatory environment surrounding the injury area. Osteoconductive composites with anti-inflammatory effects could provide a substrate to support cell growth and guide bone defect r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Polymer Testing |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0142941823001332 |
| _version_ | 1797821593418727424 |
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| author | Meixin Li Lei Xu Fenbo Ma Bin Tang Chenghe Qin |
| author_facet | Meixin Li Lei Xu Fenbo Ma Bin Tang Chenghe Qin |
| author_sort | Meixin Li |
| collection | DOAJ |
| description | Large bone defects have been a major healthcare concern. These defects are difficult to heal because of the chronic inflammatory environment surrounding the injury area. Osteoconductive composites with anti-inflammatory effects could provide a substrate to support cell growth and guide bone defect repair. Here, we synthesized a biofunctional composite that combined carboxymethyl-chitosan (CMCS) and strontium chondroitin sulfate (SrCS), for potential use in bone regeneration. We hypothesized that the as-prepared CMCS-SrCS composites could stimulate osteogenic activity and suppress inflammatory responses. The resultant composites displayed uniformly porous structures and good swelling capacity. The effect of the composites on MC3T3-E1 cells was investigated in vitro and the live cells showed favorable viability. Moreover, the RT-PCR results indicated that the CMCS-SrCS composites could suppress inflammatory responses and promote the osteogenic differentiation of MC3T3-E1 cells. These results suggested the CMCS-SrCS composites should have osteoconductive characteristics. Thus, we believe that the developed CMCS-SrCS composites should be a promising skeletal candidate for regenerative engineering applications. |
| first_indexed | 2024-03-13T09:55:03Z |
| format | Article |
| id | doaj.art-d3d4906dd70c4e559f5f70c09b60387e |
| institution | Directory Open Access Journal |
| issn | 0142-9418 |
| language | English |
| last_indexed | 2024-03-13T09:55:03Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Polymer Testing |
| spelling | doaj.art-d3d4906dd70c4e559f5f70c09b60387e2023-05-24T04:19:52ZengElsevierPolymer Testing0142-94182023-06-01123108053Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regenerationMeixin Li0Lei Xu1Fenbo Ma2Bin Tang3Chenghe Qin4Department of Orthopedics and Traumatology, Guangdong Second Provincial General Hospital, The Second Clinical Medical School of Southern Medical University, 466 Xingang Road C., Haizhu District, Guangzhou, 510317, China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Orthopedics and Traumatology, Guangdong Second Provincial General Hospital, The Second Clinical Medical School of Southern Medical University, 466 Xingang Road C., Haizhu District, Guangzhou, 510317, ChinaDepartment of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China; Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, China; Shenzhen Key Laboratory of Cell Microenvironment, China; Corresponding author. Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.Department of Orthopedics and Traumatology, Guangdong Second Provincial General Hospital, The Second Clinical Medical School of Southern Medical University, 466 Xingang Road C., Haizhu District, Guangzhou, 510317, China; Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, The First Clinical Medical School of Southern Medical University, 1838 Guangzhou N., Baiyun District, Guangzhou, 510515, China; Corresponding author. Department of Orthopedics and Traumatology, Guangdong Second Provincial General Hospital, The Second Clinical Medical School of Southern Medical University, 466 Xingang Road C., Haizhu District, Guangzhou, 510317, China.Large bone defects have been a major healthcare concern. These defects are difficult to heal because of the chronic inflammatory environment surrounding the injury area. Osteoconductive composites with anti-inflammatory effects could provide a substrate to support cell growth and guide bone defect repair. Here, we synthesized a biofunctional composite that combined carboxymethyl-chitosan (CMCS) and strontium chondroitin sulfate (SrCS), for potential use in bone regeneration. We hypothesized that the as-prepared CMCS-SrCS composites could stimulate osteogenic activity and suppress inflammatory responses. The resultant composites displayed uniformly porous structures and good swelling capacity. The effect of the composites on MC3T3-E1 cells was investigated in vitro and the live cells showed favorable viability. Moreover, the RT-PCR results indicated that the CMCS-SrCS composites could suppress inflammatory responses and promote the osteogenic differentiation of MC3T3-E1 cells. These results suggested the CMCS-SrCS composites should have osteoconductive characteristics. Thus, we believe that the developed CMCS-SrCS composites should be a promising skeletal candidate for regenerative engineering applications.http://www.sciencedirect.com/science/article/pii/S0142941823001332Carboxymethyl chitosanStrontium chondroitin sulfateBone regeneration |
| spellingShingle | Meixin Li Lei Xu Fenbo Ma Bin Tang Chenghe Qin Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration Polymer Testing Carboxymethyl chitosan Strontium chondroitin sulfate Bone regeneration |
| title | Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration |
| title_full | Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration |
| title_fullStr | Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration |
| title_full_unstemmed | Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration |
| title_short | Fabrication of carboxymethyl chitosan-strontium chondroitin sulfate composites for potential bone regeneration |
| title_sort | fabrication of carboxymethyl chitosan strontium chondroitin sulfate composites for potential bone regeneration |
| topic | Carboxymethyl chitosan Strontium chondroitin sulfate Bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0142941823001332 |
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