Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products
We investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Stap...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2014-08-01
|
| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201404-0004_prevalence-and-growth-dynamics-of-enterotoxinogenic-staphylococcus-aureus-isolates-in-slovakian-dairy-products.php |
| _version_ | 1797899709601284096 |
|---|---|
| author | Alžbeta Medveďová Adriana Studeničová Ľubomír Valík Zuzana Horváthová |
| author_facet | Alžbeta Medveďová Adriana Studeničová Ľubomír Valík Zuzana Horváthová |
| author_sort | Alžbeta Medveďová |
| collection | DOAJ |
| description | We investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Staphylococcus, and PCR detection. The PCR results revealed that 32% of S. aureus isolates possessed the selected gene for SEs enterotoxins (SEA-SEE), with sea as the most predominant gene. Neither seb nor sed genes were found throughout the collection. Additionally, the growth analysis of the isolates was performed in milk at 15°C. The growth parameters were very close to one another and were also compared with the data described in the global databases. These results provided evidence that the growth rate and lag phase duration can be determined with a high degree of reproducibility without regardless of the strain origin. |
| first_indexed | 2024-04-10T08:34:13Z |
| format | Article |
| id | doaj.art-d3d4961474ff412cad48d8d3519924f6 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:34:13Z |
| publishDate | 2014-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-d3d4961474ff412cad48d8d3519924f62023-02-23T03:28:07ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172014-08-0132433734110.17221/421/2013-CJFScjf-201404-0004Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy productsAlžbeta Medveďová0Adriana Studeničová1Ľubomír Valík2Zuzana Horváthová3Department of Nutrition and Food Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak RepublicDepartment of Nutrition and Food Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak RepublicDepartment of Nutrition and Food Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak RepublicDepartment of Nutrition and Food Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak RepublicWe investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Staphylococcus, and PCR detection. The PCR results revealed that 32% of S. aureus isolates possessed the selected gene for SEs enterotoxins (SEA-SEE), with sea as the most predominant gene. Neither seb nor sed genes were found throughout the collection. Additionally, the growth analysis of the isolates was performed in milk at 15°C. The growth parameters were very close to one another and were also compared with the data described in the global databases. These results provided evidence that the growth rate and lag phase duration can be determined with a high degree of reproducibility without regardless of the strain origin.https://cjfs.agriculturejournals.cz/artkey/cjf-201404-0004_prevalence-and-growth-dynamics-of-enterotoxinogenic-staphylococcus-aureus-isolates-in-slovakian-dairy-products.phpcoagulaso-positive staphylococcistaphylococcal enterotoxinspcrgrowth dynamic |
| spellingShingle | Alžbeta Medveďová Adriana Studeničová Ľubomír Valík Zuzana Horváthová Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products Czech Journal of Food Sciences coagulaso-positive staphylococci staphylococcal enterotoxins pcr growth dynamic |
| title | Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products |
| title_full | Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products |
| title_fullStr | Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products |
| title_full_unstemmed | Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products |
| title_short | Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products |
| title_sort | prevalence and growth dynamics of enterotoxinogenic staphylococcus aureus isolates in slovakian dairy products |
| topic | coagulaso-positive staphylococci staphylococcal enterotoxins pcr growth dynamic |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201404-0004_prevalence-and-growth-dynamics-of-enterotoxinogenic-staphylococcus-aureus-isolates-in-slovakian-dairy-products.php |
| work_keys_str_mv | AT alzbetamedvedova prevalenceandgrowthdynamicsofenterotoxinogenicstaphylococcusaureusisolatesinslovakiandairyproducts AT adrianastudenicova prevalenceandgrowthdynamicsofenterotoxinogenicstaphylococcusaureusisolatesinslovakiandairyproducts AT lubomirvalik prevalenceandgrowthdynamicsofenterotoxinogenicstaphylococcusaureusisolatesinslovakiandairyproducts AT zuzanahorvathova prevalenceandgrowthdynamicsofenterotoxinogenicstaphylococcusaureusisolatesinslovakiandairyproducts |