Effect of Electron-Beam Irradiation on Enzyme Activities in Agaricus brunnescens
This project was carried out to study the effect of five different doses of electron-beam irradiation, including: 0 as control, 1.5, 3.5, 5.5 and 7.5 kGy on peroxidase (POD), superoxide dismutase (SOD) and polyphenol oxidase (PPO) activities of brown button mushroom (Agaricus brunnescens). The ex...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-09-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/effect-of-electron-beam-irradiation-on-enzyme-activities-in-agaricus-brunnescens/ |
| Summary: | This project was carried out to study the effect of five different doses of electron-beam
irradiation, including: 0 as control, 1.5, 3.5, 5.5 and 7.5 kGy on peroxidase (POD), superoxide
dismutase (SOD) and polyphenol oxidase (PPO) activities of brown button mushroom (Agaricus
brunnescens). The experiment was conducted using an electron beam accelerator (ESS-010-03)
and measurements were made during 1, 4th, 8th, 12th and 16th day storage at 4 °C and 80 percent
relative humidity. There was a significant difference between irradiated and non-irradiated
(control) mushrooms in different enzymes (P<0.01). Most Peroxidase activity was observed
in mushrooms treated with 7.5 kGy. The irradiated mushroom with 7.5 kGy also showed the
highest SOD activity. The lowest SOD activity in mushrooms was related to control in all days
of storage. The mushrooms irradiated with 0 and 7.5 kGy contained higher and lower PPO
activity using pyrocatechol as substrate respectively, during 12th and 16th day compared with
other doses. Treatment of 0 kGy induced the highest PPO activity using pyrogallol as substrate
in storage days. The data increased the current understanding of the effects of electron-beam
irradiation on the enzyme activity changes associated with postharvest senescence and should
lead to more targeted strategies for reducing postharvest quality loss in A. brunnescens. |
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| ISSN: | 0973-7510 2581-690X |