Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections
Aims: This study aimed to characterize 79 <i>Cutibacterium acnes</i> strains isolated from prosthetic joint infections (PJIs) originated from eight European hospitals. Methods: Isolates were phylotyped according to the single-locus sequence typing (SLST) scheme. We evaluated the ability...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/9/1260 |
| _version_ | 1827664079570862080 |
|---|---|
| author | Llanos Salar-Vidal John Jairo Aguilera-Correa Holger Brüggemann Yvonne Achermann Jaime Esteban |
| author_facet | Llanos Salar-Vidal John Jairo Aguilera-Correa Holger Brüggemann Yvonne Achermann Jaime Esteban |
| author_sort | Llanos Salar-Vidal |
| collection | DOAJ |
| description | Aims: This study aimed to characterize 79 <i>Cutibacterium acnes</i> strains isolated from prosthetic joint infections (PJIs) originated from eight European hospitals. Methods: Isolates were phylotyped according to the single-locus sequence typing (SLST) scheme. We evaluated the ability of the biofilm formation of <i>C. acnes</i> strains isolated from PJIs and 84 isolates recovered from healthy skin. Antibiotic susceptibility testing of planktonic and biofilm cells of PJI isolates and skin isolates was performed. Results: Most of the isolates from PJIs belonged to the SLST class H/phylotype IB (34.2%), followed by class D/phylotype IA<sub>1</sub> (21.5%), class A/phylotype IA<sub>1</sub> (18.9%), and class K/phylotype II (13.9%). All tested isolates were biofilm producers; no difference in biofilm formation was observed between the healthy skin group and the PJI group of strains. Planktonic and sessile cells of <i>C. acnes</i> remained highly susceptible to a broad spectrum of antibiotics, including beta-lactams, clindamycin, fluoroquinolones, linezolid, rifampin, and vancomycin. The minimal inhibitory concentrations (MICs) for planktonic and biofilm states coincided in most cases. However, the minimal biofilm eradication concentration (MBEC) was high for all antimicrobial drugs tested (>32 mg/L), except for rifampin (2 mg/L). Conclusions: <i>C. acnes</i> strains isolated from healthy skin were able to produce biofilm to the same extent as isolates recovered from PJIs. All <i>C. acnes</i> strains in planktonic and sessile states were susceptible to most antibiotics commonly used for PJI treatment, although rifampin was the only antimicrobial agent able to eradicate <i>C. acnes</i> embedded in biofilm. |
| first_indexed | 2024-03-10T00:55:10Z |
| format | Article |
| id | doaj.art-d3d76dcdd63d420ebda101b1e1bcfa4b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T00:55:10Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d3d76dcdd63d420ebda101b1e1bcfa4b2023-11-23T14:45:46ZengMDPI AGAntibiotics2079-63822022-09-01119126010.3390/antibiotics11091260Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint InfectionsLlanos Salar-Vidal0John Jairo Aguilera-Correa1Holger Brüggemann2Yvonne Achermann3Jaime Esteban4Clinical Microbiology Department, IIS-Fundación Jiménez Díaz, 28020 Madrid, SpainClinical Microbiology Department, IIS-Fundación Jiménez Díaz, 28020 Madrid, SpainDepartment of Biomedicine, Aarhus University, 8000 Aarhus, DenmarkDivision of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich (USZ), 8091 Zurich, SwitzerlandClinical Microbiology Department, IIS-Fundación Jiménez Díaz, 28020 Madrid, SpainAims: This study aimed to characterize 79 <i>Cutibacterium acnes</i> strains isolated from prosthetic joint infections (PJIs) originated from eight European hospitals. Methods: Isolates were phylotyped according to the single-locus sequence typing (SLST) scheme. We evaluated the ability of the biofilm formation of <i>C. acnes</i> strains isolated from PJIs and 84 isolates recovered from healthy skin. Antibiotic susceptibility testing of planktonic and biofilm cells of PJI isolates and skin isolates was performed. Results: Most of the isolates from PJIs belonged to the SLST class H/phylotype IB (34.2%), followed by class D/phylotype IA<sub>1</sub> (21.5%), class A/phylotype IA<sub>1</sub> (18.9%), and class K/phylotype II (13.9%). All tested isolates were biofilm producers; no difference in biofilm formation was observed between the healthy skin group and the PJI group of strains. Planktonic and sessile cells of <i>C. acnes</i> remained highly susceptible to a broad spectrum of antibiotics, including beta-lactams, clindamycin, fluoroquinolones, linezolid, rifampin, and vancomycin. The minimal inhibitory concentrations (MICs) for planktonic and biofilm states coincided in most cases. However, the minimal biofilm eradication concentration (MBEC) was high for all antimicrobial drugs tested (>32 mg/L), except for rifampin (2 mg/L). Conclusions: <i>C. acnes</i> strains isolated from healthy skin were able to produce biofilm to the same extent as isolates recovered from PJIs. All <i>C. acnes</i> strains in planktonic and sessile states were susceptible to most antibiotics commonly used for PJI treatment, although rifampin was the only antimicrobial agent able to eradicate <i>C. acnes</i> embedded in biofilm.https://www.mdpi.com/2079-6382/11/9/1260<i>Cutibacteriumacnes</i>prosthetic joint infectionbiofilmantimicrobial susceptibilityphylotyping |
| spellingShingle | Llanos Salar-Vidal John Jairo Aguilera-Correa Holger Brüggemann Yvonne Achermann Jaime Esteban Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections Antibiotics <i>Cutibacteriumacnes</i> prosthetic joint infection biofilm antimicrobial susceptibility phylotyping |
| title | Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections |
| title_full | Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections |
| title_fullStr | Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections |
| title_full_unstemmed | Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections |
| title_short | Microbiological Characterization of <i>Cutibacterium acnes</i> Strains Isolated from Prosthetic Joint Infections |
| title_sort | microbiological characterization of i cutibacterium acnes i strains isolated from prosthetic joint infections |
| topic | <i>Cutibacteriumacnes</i> prosthetic joint infection biofilm antimicrobial susceptibility phylotyping |
| url | https://www.mdpi.com/2079-6382/11/9/1260 |
| work_keys_str_mv | AT llanossalarvidal microbiologicalcharacterizationoficutibacteriumacnesistrainsisolatedfromprostheticjointinfections AT johnjairoaguileracorrea microbiologicalcharacterizationoficutibacteriumacnesistrainsisolatedfromprostheticjointinfections AT holgerbruggemann microbiologicalcharacterizationoficutibacteriumacnesistrainsisolatedfromprostheticjointinfections AT yvonneachermann microbiologicalcharacterizationoficutibacteriumacnesistrainsisolatedfromprostheticjointinfections AT jaimeesteban microbiologicalcharacterizationoficutibacteriumacnesistrainsisolatedfromprostheticjointinfections |