The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems
Background: Okadaic acid (OA) is a toxin of polluted shellfish. Consuming the contaminated shellfish is accompanied by diarrhea and paralytic and amnesic disorders. There is a correlation between diarrhea and the consumed OA. Determining the critical targeted genes by OA was the aim of this study....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Shaheed Beheshti University of Medical Sciences
2023-12-01
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| Series: | International Journal of Medical Toxicology and Forensic Medicine |
| Subjects: | |
| Online Access: | https://journals.sbmu.ac.ir/ijmtfm/article/view/42997 |
| _version_ | 1827353599370330112 |
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| author | Reza M Robati Zahra Razzaghi Babak Arjmand Mostafa Rezaei Tavirani Mohammad Rostami Nejad Mitra Rezaei Mona Zamanian Azodi |
| author_facet | Reza M Robati Zahra Razzaghi Babak Arjmand Mostafa Rezaei Tavirani Mohammad Rostami Nejad Mitra Rezaei Mona Zamanian Azodi |
| author_sort | Reza M Robati |
| collection | DOAJ |
| description | Background: Okadaic acid (OA) is a toxin of polluted shellfish. Consuming the contaminated shellfish is accompanied by diarrhea and paralytic and amnesic disorders. There is a correlation between diarrhea and the consumed OA. Determining the critical targeted genes by OA was the aim of this study.
Methods: The transcriptomic data about the effect of OA on human intestinal caco-2 cells were extracted from gene expression omnibus (GEO) and evaluated via the GEO2R program. The significant differentially expressed genes (DEGs) were included in a protein-protein interaction (PPI) network and the central nodes were enriched via gene ontology to find the crucial affected biological terms.
Results: Among the 178 significant DEGs plus 50 added first neighbors, four hub-bottleneck genes (ALB, FOS, JUN, and MYC) were determined. Twenty-eight critical biological terms were identified as the dysregulated individuals in response to the presence of OA. “ERK1/2-activator protein-1 (AP-1) complex binds KDM6B promoter” was highlighted as the major class of biological terms.
Conclusion: It can be concluded that down-regulation of ALB as a potent central gene leads to impairment of blood homeostasis in the presence of OA. Up-regulation of the other three central genes (JUN, FOS, and MYC) grossly affects the vital pathways in the human body. |
| first_indexed | 2024-03-08T03:28:48Z |
| format | Article |
| id | doaj.art-d3daf50b4de444af8734c05ecaf9bece |
| institution | Directory Open Access Journal |
| issn | 2251-8762 2251-8770 |
| language | English |
| last_indexed | 2024-03-08T03:28:48Z |
| publishDate | 2023-12-01 |
| publisher | Shaheed Beheshti University of Medical Sciences |
| record_format | Article |
| series | International Journal of Medical Toxicology and Forensic Medicine |
| spelling | doaj.art-d3daf50b4de444af8734c05ecaf9bece2024-02-11T07:38:34ZengShaheed Beheshti University of Medical SciencesInternational Journal of Medical Toxicology and Forensic Medicine2251-87622251-87702023-12-01134429974299710.32598/ijmtfm.v13i4.4299732803The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological SystemsReza M Robati0Zahra Razzaghi1Babak Arjmand2Mostafa Rezaei Tavirani3Mohammad Rostami Nejad4Mitra Rezaei5Mona Zamanian Azodi6Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran, Iranian Cancer Control Center (MACSA), Tehran, Iran.Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Background: Okadaic acid (OA) is a toxin of polluted shellfish. Consuming the contaminated shellfish is accompanied by diarrhea and paralytic and amnesic disorders. There is a correlation between diarrhea and the consumed OA. Determining the critical targeted genes by OA was the aim of this study. Methods: The transcriptomic data about the effect of OA on human intestinal caco-2 cells were extracted from gene expression omnibus (GEO) and evaluated via the GEO2R program. The significant differentially expressed genes (DEGs) were included in a protein-protein interaction (PPI) network and the central nodes were enriched via gene ontology to find the crucial affected biological terms. Results: Among the 178 significant DEGs plus 50 added first neighbors, four hub-bottleneck genes (ALB, FOS, JUN, and MYC) were determined. Twenty-eight critical biological terms were identified as the dysregulated individuals in response to the presence of OA. “ERK1/2-activator protein-1 (AP-1) complex binds KDM6B promoter” was highlighted as the major class of biological terms. Conclusion: It can be concluded that down-regulation of ALB as a potent central gene leads to impairment of blood homeostasis in the presence of OA. Up-regulation of the other three central genes (JUN, FOS, and MYC) grossly affects the vital pathways in the human body.https://journals.sbmu.ac.ir/ijmtfm/article/view/42997okadaic acidgene expressioncentral genegene ontologybiological term |
| spellingShingle | Reza M Robati Zahra Razzaghi Babak Arjmand Mostafa Rezaei Tavirani Mohammad Rostami Nejad Mitra Rezaei Mona Zamanian Azodi The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems International Journal of Medical Toxicology and Forensic Medicine okadaic acid gene expression central gene gene ontology biological term |
| title | The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems |
| title_full | The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems |
| title_fullStr | The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems |
| title_full_unstemmed | The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems |
| title_short | The Main Targets of Okadaic Acid Toxin in Human Intestinal Caco-2 Cells: An Investigation of Biological Systems |
| title_sort | main targets of okadaic acid toxin in human intestinal caco 2 cells an investigation of biological systems |
| topic | okadaic acid gene expression central gene gene ontology biological term |
| url | https://journals.sbmu.ac.ir/ijmtfm/article/view/42997 |
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