PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII
Introduction: Photosystem II (PSII) protein complex plays an essential role in the entire photosynthesis process. Various known and unknown protein factors are involved in the dynamics of the PSII complex that need to be characterized in crop plants for enhancing photosynthesis efficiency and produc...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123220302344 |
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| author | M. Raghurami Reddy Satendra K. Mangrauthia S. Venkata Reddy P. Manimaran Poli Yugandhar P. Naresh Babu T. Vishnukiran D. Subrahmanyam R.M. Sundaram S.M. Balachandran |
| author_facet | M. Raghurami Reddy Satendra K. Mangrauthia S. Venkata Reddy P. Manimaran Poli Yugandhar P. Naresh Babu T. Vishnukiran D. Subrahmanyam R.M. Sundaram S.M. Balachandran |
| author_sort | M. Raghurami Reddy |
| collection | DOAJ |
| description | Introduction: Photosystem II (PSII) protein complex plays an essential role in the entire photosynthesis process. Various known and unknown protein factors are involved in the dynamics of the PSII complex that need to be characterized in crop plants for enhancing photosynthesis efficiency and productivity. Objectives: The experiments were conducted to decipher the regulatory proteins involved in PSII dynamics of rice crop. Methods: A novel rice regulatory protein PAP90 (PSII auxiliary protein ~90 kDa) was characterized by generating a loss-of-function mutant pap90. The mutation was characterized at molecular level followed by various experiments to analyze the morphological, physiological and biochemical processes of mutant under control and abiotic stresses. Results: The pap90 mutant showed reduced photosynthesis due to D1 protein instability that subsequently causes inadequate accumulation of thylakoid membrane complexes, especially PSII and decreases PSII functional efficiency. Expression of OsFtsH family genes and proteins were induced in the mutant, which are known to play a key role in D1 protein degradation and turnover. The reduced D1 protein accumulation in the mutant increased the production of reactive oxygen species (ROS). The accumulation of ROS along with the increased activity of antioxidant enzymes and induced expression of stress-associated genes and proteins in pap90 mutant contributed to its water-limited stress tolerance ability. Conclusion: We propose that PAP90 is a key auxiliary protein that interacts with D1 protein and maintains its stability, thereby promoting subsequent assembly of the PSII and associated membrane complexes. |
| first_indexed | 2024-12-17T01:44:34Z |
| format | Article |
| id | doaj.art-d3ddfdd5a2be489985a586eb955b05d4 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-12-17T01:44:34Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-d3ddfdd5a2be489985a586eb955b05d42022-12-21T22:08:14ZengElsevierJournal of Advanced Research2090-12322021-05-0130197211PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSIIM. Raghurami Reddy0Satendra K. Mangrauthia1S. Venkata Reddy2P. Manimaran3Poli Yugandhar4P. Naresh Babu5T. Vishnukiran6D. Subrahmanyam7R.M. Sundaram8S.M. Balachandran9Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaBiotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India; Corresponding authors.Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, IndiaDepartment of Plant Sciences, University of Hyderabad, Hyderabad 500046, IndiaPhysiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaBiotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaPhysiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaPhysiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaBiotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, IndiaBiotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India; Corresponding authors.Introduction: Photosystem II (PSII) protein complex plays an essential role in the entire photosynthesis process. Various known and unknown protein factors are involved in the dynamics of the PSII complex that need to be characterized in crop plants for enhancing photosynthesis efficiency and productivity. Objectives: The experiments were conducted to decipher the regulatory proteins involved in PSII dynamics of rice crop. Methods: A novel rice regulatory protein PAP90 (PSII auxiliary protein ~90 kDa) was characterized by generating a loss-of-function mutant pap90. The mutation was characterized at molecular level followed by various experiments to analyze the morphological, physiological and biochemical processes of mutant under control and abiotic stresses. Results: The pap90 mutant showed reduced photosynthesis due to D1 protein instability that subsequently causes inadequate accumulation of thylakoid membrane complexes, especially PSII and decreases PSII functional efficiency. Expression of OsFtsH family genes and proteins were induced in the mutant, which are known to play a key role in D1 protein degradation and turnover. The reduced D1 protein accumulation in the mutant increased the production of reactive oxygen species (ROS). The accumulation of ROS along with the increased activity of antioxidant enzymes and induced expression of stress-associated genes and proteins in pap90 mutant contributed to its water-limited stress tolerance ability. Conclusion: We propose that PAP90 is a key auxiliary protein that interacts with D1 protein and maintains its stability, thereby promoting subsequent assembly of the PSII and associated membrane complexes.http://www.sciencedirect.com/science/article/pii/S2090123220302344Abiotic stressD1 proteinOryza sativa L.OsFtsHPhotosystem IIROS |
| spellingShingle | M. Raghurami Reddy Satendra K. Mangrauthia S. Venkata Reddy P. Manimaran Poli Yugandhar P. Naresh Babu T. Vishnukiran D. Subrahmanyam R.M. Sundaram S.M. Balachandran PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII Journal of Advanced Research Abiotic stress D1 protein Oryza sativa L. OsFtsH Photosystem II ROS |
| title | PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII |
| title_full | PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII |
| title_fullStr | PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII |
| title_full_unstemmed | PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII |
| title_short | PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII |
| title_sort | pap90 a novel rice protein plays a critical role in regulation of d1 protein stability of psii |
| topic | Abiotic stress D1 protein Oryza sativa L. OsFtsH Photosystem II ROS |
| url | http://www.sciencedirect.com/science/article/pii/S2090123220302344 |
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