Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran
Cryptosporidium is an intracellular and extracytoplasmic protozoan that belongs to the phylum Apicomplexa. In this observational study, fecal samples were randomly collected from 800 dairy cattle, in 10 industrial dairy farms in Mashhad, Iran (from 2011 to 2015 years). The presence of Cryptosporidiu...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2020-04-01
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| Series: | Veterinaria Italiana |
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| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1771 |
| _version_ | 1798023959681171456 |
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| author | Alireza Sadrebazzaz |
| author_facet | Alireza Sadrebazzaz |
| author_sort | Alireza Sadrebazzaz |
| collection | DOAJ |
| description | Cryptosporidium is an intracellular and extracytoplasmic protozoan that belongs to the phylum Apicomplexa. In this observational study, fecal samples were randomly collected from 800 dairy cattle, in 10 industrial dairy farms in Mashhad, Iran (from 2011 to 2015 years). The presence of Cryptosporidium oocysts was determined by modified cold Ziehl‑Neelsen’s staining. Results of microscopy observation showed that 23 samples (2.87%) were positive for Cryptosporidium oocyst. Twenty two samples were confirmed by PCR. The identification of
Cryptosporidium andersoni was determined by restriction digestions of PCR products, using SspI, VspI, and DdeI enzymes. Differences between healthy and diarrheic groups as well as between age groups were not observed. |
| first_indexed | 2024-04-11T17:54:46Z |
| format | Article |
| id | doaj.art-d3de7b1421cb404ca86ffd89f6bfa496 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-11T17:54:46Z |
| publishDate | 2020-04-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-d3de7b1421cb404ca86ffd89f6bfa4962022-12-22T04:10:51ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272020-04-0156110.12834/VetIt.1771.9343.3Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of IranAlireza Sadrebazzaz0Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension OrganizationCryptosporidium is an intracellular and extracytoplasmic protozoan that belongs to the phylum Apicomplexa. In this observational study, fecal samples were randomly collected from 800 dairy cattle, in 10 industrial dairy farms in Mashhad, Iran (from 2011 to 2015 years). The presence of Cryptosporidium oocysts was determined by modified cold Ziehl‑Neelsen’s staining. Results of microscopy observation showed that 23 samples (2.87%) were positive for Cryptosporidium oocyst. Twenty two samples were confirmed by PCR. The identification of Cryptosporidium andersoni was determined by restriction digestions of PCR products, using SspI, VspI, and DdeI enzymes. Differences between healthy and diarrheic groups as well as between age groups were not observed.https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1771Cryptosporidium andersoniCattlePCR‑RFLPMashhadIran |
| spellingShingle | Alireza Sadrebazzaz Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran Veterinaria Italiana Cryptosporidium andersoni Cattle PCR‑RFLP Mashhad Iran |
| title | Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran |
| title_full | Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran |
| title_fullStr | Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran |
| title_full_unstemmed | Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran |
| title_short | Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran |
| title_sort | molecular identification of cryptosporidium andersoni in healthy and in cattle with diarrhea of mashhad northeast of iran |
| topic | Cryptosporidium andersoni Cattle PCR‑RFLP Mashhad Iran |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1771 |
| work_keys_str_mv | AT alirezasadrebazzaz molecularidentificationofcryptosporidiumandersoniinhealthyandincattlewithdiarrheaofmashhadnortheastofiran |