Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress
Multiprotein bridging factor 1 (MBF1) is an ancient family of transcription coactivators that play a crucial role in the response of plants to abiotic stress. In this study, we analyzed the genomic data of five Solanaceae plants and identified a total of 21 <i>MBF1</i> genes. The expansi...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13965 |
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| author | Dongnan Xia Lulu Guan Yue Yin Yixi Wang Hongyan Shi Wenyu Li Dekai Zhang Ran Song Tixu Hu Xiangqiang Zhan |
| author_facet | Dongnan Xia Lulu Guan Yue Yin Yixi Wang Hongyan Shi Wenyu Li Dekai Zhang Ran Song Tixu Hu Xiangqiang Zhan |
| author_sort | Dongnan Xia |
| collection | DOAJ |
| description | Multiprotein bridging factor 1 (MBF1) is an ancient family of transcription coactivators that play a crucial role in the response of plants to abiotic stress. In this study, we analyzed the genomic data of five Solanaceae plants and identified a total of 21 <i>MBF1</i> genes. The expansion of MBF1a and MBF1b subfamilies was attributed to whole-genome duplication (WGD), and the expansion of the MBF1c subfamily occurred through transposed duplication (TRD). Collinearity analysis within Solanaceae species revealed collinearity between members of the MBF1a and MBF1b subfamilies, whereas the MBF1c subfamily showed relative independence. The gene expression of <i>SlER24</i> was induced by sodium chloride (NaCl), polyethylene glycol (PEG), ABA (abscisic acid), and ethrel treatments, with the highest expression observed under NaCl treatment. The overexpression of <i>SlER24</i> significantly enhanced the salt tolerance of tomato, and the functional deficiency of <i>SlER24</i> decreased the tolerance of tomato to salt stress. <i>SlER24</i> enhanced antioxidant enzyme activity to reduce the accumulation of reactive oxygen species (ROS) and alleviated plasma membrane damage under salt stress. <i>SlER24</i> upregulated the expression levels of salt stress-related genes to enhance salt tolerance in tomato. In conclusion, this study provides basic information for the study of the <i>MBF1</i> family of Solanaceae under abiotic stress, as well as a reference for the study of other plants. |
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| language | English |
| last_indexed | 2024-03-10T22:40:39Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d3e0ddb205af4508aa80d346bf5ef4722023-11-19T11:05:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181396510.3390/ijms241813965Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt StressDongnan Xia0Lulu Guan1Yue Yin2Yixi Wang3Hongyan Shi4Wenyu Li5Dekai Zhang6Ran Song7Tixu Hu8Xiangqiang Zhan9State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, ChinaMultiprotein bridging factor 1 (MBF1) is an ancient family of transcription coactivators that play a crucial role in the response of plants to abiotic stress. In this study, we analyzed the genomic data of five Solanaceae plants and identified a total of 21 <i>MBF1</i> genes. The expansion of MBF1a and MBF1b subfamilies was attributed to whole-genome duplication (WGD), and the expansion of the MBF1c subfamily occurred through transposed duplication (TRD). Collinearity analysis within Solanaceae species revealed collinearity between members of the MBF1a and MBF1b subfamilies, whereas the MBF1c subfamily showed relative independence. The gene expression of <i>SlER24</i> was induced by sodium chloride (NaCl), polyethylene glycol (PEG), ABA (abscisic acid), and ethrel treatments, with the highest expression observed under NaCl treatment. The overexpression of <i>SlER24</i> significantly enhanced the salt tolerance of tomato, and the functional deficiency of <i>SlER24</i> decreased the tolerance of tomato to salt stress. <i>SlER24</i> enhanced antioxidant enzyme activity to reduce the accumulation of reactive oxygen species (ROS) and alleviated plasma membrane damage under salt stress. <i>SlER24</i> upregulated the expression levels of salt stress-related genes to enhance salt tolerance in tomato. In conclusion, this study provides basic information for the study of the <i>MBF1</i> family of Solanaceae under abiotic stress, as well as a reference for the study of other plants.https://www.mdpi.com/1422-0067/24/18/13965Solanaceae species<i>MBF1</i>gene expression patterns<i>SlER24</i>salt stress |
| spellingShingle | Dongnan Xia Lulu Guan Yue Yin Yixi Wang Hongyan Shi Wenyu Li Dekai Zhang Ran Song Tixu Hu Xiangqiang Zhan Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress International Journal of Molecular Sciences Solanaceae species <i>MBF1</i> gene expression patterns <i>SlER24</i> salt stress |
| title | Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress |
| title_full | Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress |
| title_fullStr | Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress |
| title_full_unstemmed | Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress |
| title_short | Genome-Wide Analysis of <i>MBF1</i> Family Genes in Five Solanaceous Plants and Functional Analysis of <i>SlER24</i> in Salt Stress |
| title_sort | genome wide analysis of i mbf1 i family genes in five solanaceous plants and functional analysis of i sler24 i in salt stress |
| topic | Solanaceae species <i>MBF1</i> gene expression patterns <i>SlER24</i> salt stress |
| url | https://www.mdpi.com/1422-0067/24/18/13965 |
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