Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice
The study was aimed to explore the possible function of thermal ablation treatment on T helper 17 (Th17) cells and regulatory T (Treg) cells in transplantation of hepatocellular carcinoma in mice. In total, 60 male C57BL/6 mice were divided into control group, model group, and treat group. Flow cyto...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-03-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/2058739219832489 |
| _version_ | 1818576217440256000 |
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| author | Shengchuan Huang Nina Qu Yanming Men Zhen Liu |
| author_facet | Shengchuan Huang Nina Qu Yanming Men Zhen Liu |
| author_sort | Shengchuan Huang |
| collection | DOAJ |
| description | The study was aimed to explore the possible function of thermal ablation treatment on T helper 17 (Th17) cells and regulatory T (Treg) cells in transplantation of hepatocellular carcinoma in mice. In total, 60 male C57BL/6 mice were divided into control group, model group, and treat group. Flow cytometry was used to detect the frequency of Th17 and Treg cells in peripheral blood. The levels of interleukin (IL)-17, IL-23, IL-10, and transforming growth factor beta (TGF-β) in serum were detected by enzyme-linked immunosorbent assay (ELISA).The levels of IL-17, RORγt, Foxp3, and TGF-β mRNA in tumor tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR). Compared with the model group, tumor size was significantly decreased after thermal ablation treatment. After treatment, the frequency of Th17 cells in peripheral blood was significantly decreased, while the frequency of Treg cells was profoundly increased ( P < 0.05). The levels of IL-17 and IL-23 were significantly downregulated, while IL-10 and TGF-β levels were upregulated ( P < 0.05). IL-17 and RORγt mRNA levels in tumor tissues were significantly decreased ( P < 0.05), and Foxp3 and TGF-β mRNA levels were significantly increased ( P < 0.05). Thermal ablation treatment plays a positive role in the treatment of hepatoma in mice through affecting the imbalance of Th17/Treg cells. |
| first_indexed | 2024-12-16T06:10:30Z |
| format | Article |
| id | doaj.art-d3e24abaa0974f5cbdd49291301dc65c |
| institution | Directory Open Access Journal |
| issn | 2058-7392 |
| language | English |
| last_indexed | 2024-12-16T06:10:30Z |
| publishDate | 2019-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-d3e24abaa0974f5cbdd49291301dc65c2022-12-21T22:41:25ZengSAGE PublishingEuropean Journal of Inflammation2058-73922019-03-011710.1177/2058739219832489Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of miceShengchuan HuangNina QuYanming MenZhen LiuThe study was aimed to explore the possible function of thermal ablation treatment on T helper 17 (Th17) cells and regulatory T (Treg) cells in transplantation of hepatocellular carcinoma in mice. In total, 60 male C57BL/6 mice were divided into control group, model group, and treat group. Flow cytometry was used to detect the frequency of Th17 and Treg cells in peripheral blood. The levels of interleukin (IL)-17, IL-23, IL-10, and transforming growth factor beta (TGF-β) in serum were detected by enzyme-linked immunosorbent assay (ELISA).The levels of IL-17, RORγt, Foxp3, and TGF-β mRNA in tumor tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR). Compared with the model group, tumor size was significantly decreased after thermal ablation treatment. After treatment, the frequency of Th17 cells in peripheral blood was significantly decreased, while the frequency of Treg cells was profoundly increased ( P < 0.05). The levels of IL-17 and IL-23 were significantly downregulated, while IL-10 and TGF-β levels were upregulated ( P < 0.05). IL-17 and RORγt mRNA levels in tumor tissues were significantly decreased ( P < 0.05), and Foxp3 and TGF-β mRNA levels were significantly increased ( P < 0.05). Thermal ablation treatment plays a positive role in the treatment of hepatoma in mice through affecting the imbalance of Th17/Treg cells.https://doi.org/10.1177/2058739219832489 |
| spellingShingle | Shengchuan Huang Nina Qu Yanming Men Zhen Liu Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice European Journal of Inflammation |
| title | Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice |
| title_full | Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice |
| title_fullStr | Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice |
| title_full_unstemmed | Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice |
| title_short | Effects of thermal ablation on Treg/Th17 in hepatocellular carcinoma of mice |
| title_sort | effects of thermal ablation on treg th17 in hepatocellular carcinoma of mice |
| url | https://doi.org/10.1177/2058739219832489 |
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