Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats
Abstract While the role of cytokines in celiac disease has been investigated in detail, cytokine release in the event of the exposure of healthy subjects to glutens has only recently been studied. This study was aimed at determining the effects of corn and wheat glutens, incorporated as protein sour...
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Instituto de Tecnologia do Paraná (Tecpar)
2021-09-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100315&tlng=en |
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| author | Recep Gümüş Sema Uslu Uğur Aydoğdu Aybüke İmik Mehmet Ekici |
| author_facet | Recep Gümüş Sema Uslu Uğur Aydoğdu Aybüke İmik Mehmet Ekici |
| author_sort | Recep Gümüş |
| collection | DOAJ |
| description | Abstract While the role of cytokines in celiac disease has been investigated in detail, cytokine release in the event of the exposure of healthy subjects to glutens has only recently been studied. This study was aimed at determining the effects of corn and wheat glutens, incorporated as protein sources into the diet, on serum interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels and the immunohistochemical distribution of CD3 and CD8 receptors in the small intestine in male rats. The study material comprised 24 twenty-day-old male Wistar albino rats, which were randomly assigned in equal numbers to three groups (2 rats/replicate and 4 replicates/group). The feed rations provided to all three groups contained high levels of proteins, which were soybean meal, corn gluten and wheat gluten in the control, corn and wheat groups, respectively. The in Control, Corn and Wheat groups serum IL-1 beta and TNF-alpha levels respectively 55.83 - 46.37; 81.65 - 61.95 and 81.65-61.31 was determined but these differences were statistically insignificant. Furthermore, immunohistochemical examination demonstrated a mathematical increase to have occurred in the distribution of the CD3 and CD8 receptors in the duodenum, jejunum and ileum samples of the corn and wheat groups. In result, based on the findings obtained in this study, we suggest that the long-term feeding of rats on high levels of gluten causes systemic adverse effects. |
| first_indexed | 2024-12-20T15:19:00Z |
| format | Article |
| id | doaj.art-d3e40fc6082b47398bfc3dcb917b94db |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-20T15:19:00Z |
| publishDate | 2021-09-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-d3e40fc6082b47398bfc3dcb917b94db2022-12-21T19:36:07ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242021-09-016410.1590/1678-4324-2021210256Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male RatsRecep Gümüşhttps://orcid.org/0000-0002-8812-191XSema Usluhttps://orcid.org/0000-0002-2239-7841Uğur Aydoğduhttps://orcid.org/0000-0002-9828-9863Aybüke İmikhttps://orcid.org/0000-0003-4697-812XMehmet Ekicihttps://orcid.org/0000-0002-2163-6214Abstract While the role of cytokines in celiac disease has been investigated in detail, cytokine release in the event of the exposure of healthy subjects to glutens has only recently been studied. This study was aimed at determining the effects of corn and wheat glutens, incorporated as protein sources into the diet, on serum interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels and the immunohistochemical distribution of CD3 and CD8 receptors in the small intestine in male rats. The study material comprised 24 twenty-day-old male Wistar albino rats, which were randomly assigned in equal numbers to three groups (2 rats/replicate and 4 replicates/group). The feed rations provided to all three groups contained high levels of proteins, which were soybean meal, corn gluten and wheat gluten in the control, corn and wheat groups, respectively. The in Control, Corn and Wheat groups serum IL-1 beta and TNF-alpha levels respectively 55.83 - 46.37; 81.65 - 61.95 and 81.65-61.31 was determined but these differences were statistically insignificant. Furthermore, immunohistochemical examination demonstrated a mathematical increase to have occurred in the distribution of the CD3 and CD8 receptors in the duodenum, jejunum and ileum samples of the corn and wheat groups. In result, based on the findings obtained in this study, we suggest that the long-term feeding of rats on high levels of gluten causes systemic adverse effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100315&tlng=encytokineglutenimmunohistochemicalrat |
| spellingShingle | Recep Gümüş Sema Uslu Uğur Aydoğdu Aybüke İmik Mehmet Ekici Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats Brazilian Archives of Biology and Technology cytokine gluten immunohistochemical rat |
| title | Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats |
| title_full | Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats |
| title_fullStr | Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats |
| title_full_unstemmed | Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats |
| title_short | Investigation of the Effects of Glutens on Serum Interleukin-1 Beta and Tumor Necrosis Factor-Alpha Levels and the Immunohistochemical Distribution of CD3 and CD8 Receptors in the Small Intestine in Male Rats |
| title_sort | investigation of the effects of glutens on serum interleukin 1 beta and tumor necrosis factor alpha levels and the immunohistochemical distribution of cd3 and cd8 receptors in the small intestine in male rats |
| topic | cytokine gluten immunohistochemical rat |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100315&tlng=en |
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