The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination
Abstract Background Seed germination, the foundation of plant propagation, involves a series of changes at the molecular level. Poplar is a model woody plant, but the molecular events occurring during seed germination in this species are unclear. Results In this study, we investigated changes in gen...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-6180-5 |
| _version_ | 1818523327751258112 |
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| author | Chunpu Qu Hancheng Zhao Jinyuan Chen Zhuang Zuo Xue Sun Jiahuan Huang Chengjun Yang Xiuli Zhang Peng Zhang Xiankui Quan Zhiru Xu Guanjun Liu |
| author_facet | Chunpu Qu Hancheng Zhao Jinyuan Chen Zhuang Zuo Xue Sun Jiahuan Huang Chengjun Yang Xiuli Zhang Peng Zhang Xiankui Quan Zhiru Xu Guanjun Liu |
| author_sort | Chunpu Qu |
| collection | DOAJ |
| description | Abstract Background Seed germination, the foundation of plant propagation, involves a series of changes at the molecular level. Poplar is a model woody plant, but the molecular events occurring during seed germination in this species are unclear. Results In this study, we investigated changes in gene transcriptional levels during different germination periods in poplar by high-throughput sequencing technology. Analysis of genes expressed at specific germination stages indicated that these genes are distributed in many metabolic pathways. Enrichment analysis of significantly differentially expressed genes based on hypergeometric testing revealed that multiple pathways, such as pathways related to glycolysis, lipid, amino acid, protein and ATP synthesis metabolism, changed significantly at the transcriptional level during seed germination. A comparison of ΣZ values uncovered a series of transcriptional changes in biological processes related to primary metabolism during poplar seed germination. Among these changes, genes related to CHO metabolism were the first to be activated, with subsequent expression of genes involved in lipid metabolism and then those associated with protein metabolism. The pattern of metabolomic and physiological index changes further verified the sequence of some biological events. Conclusions Our study revealed molecular events occurring at the transcriptional level during seed germination and determined their order. These events were further verified by patterns of changes of metabolites and physiological indexes. Our findings lay a foundation for the elucidation of the molecular mechanisms responsible for poplar seed germination. |
| first_indexed | 2024-12-11T05:43:38Z |
| format | Article |
| id | doaj.art-d3e42de5b91d49c89e8bc0186c06849e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T05:43:38Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-d3e42de5b91d49c89e8bc0186c06849e2022-12-22T01:19:02ZengBMCBMC Genomics1471-21642019-11-0120111310.1186/s12864-019-6180-5The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germinationChunpu Qu0Hancheng Zhao1Jinyuan Chen2Zhuang Zuo3Xue Sun4Jiahuan Huang5Chengjun Yang6Xiuli Zhang7Peng Zhang8Xiankui Quan9Zhiru Xu10Guanjun Liu11State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityCollege of Life Science, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityCollege of Life Science, Northeast Forestry UniversitySchool of Forestry, Northeast Forestry UniversityCollege of Life Science, Northeast Forestry UniversitySchool of Forestry, Northeast Forestry UniversitySchool of Forestry, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry UniversityAbstract Background Seed germination, the foundation of plant propagation, involves a series of changes at the molecular level. Poplar is a model woody plant, but the molecular events occurring during seed germination in this species are unclear. Results In this study, we investigated changes in gene transcriptional levels during different germination periods in poplar by high-throughput sequencing technology. Analysis of genes expressed at specific germination stages indicated that these genes are distributed in many metabolic pathways. Enrichment analysis of significantly differentially expressed genes based on hypergeometric testing revealed that multiple pathways, such as pathways related to glycolysis, lipid, amino acid, protein and ATP synthesis metabolism, changed significantly at the transcriptional level during seed germination. A comparison of ΣZ values uncovered a series of transcriptional changes in biological processes related to primary metabolism during poplar seed germination. Among these changes, genes related to CHO metabolism were the first to be activated, with subsequent expression of genes involved in lipid metabolism and then those associated with protein metabolism. The pattern of metabolomic and physiological index changes further verified the sequence of some biological events. Conclusions Our study revealed molecular events occurring at the transcriptional level during seed germination and determined their order. These events were further verified by patterns of changes of metabolites and physiological indexes. Our findings lay a foundation for the elucidation of the molecular mechanisms responsible for poplar seed germination.http://link.springer.com/article/10.1186/s12864-019-6180-5PoplarSeed germination and post-germinationRNA-SeqMetabolite changesTranscriptional timetablePhysiological index |
| spellingShingle | Chunpu Qu Hancheng Zhao Jinyuan Chen Zhuang Zuo Xue Sun Jiahuan Huang Chengjun Yang Xiuli Zhang Peng Zhang Xiankui Quan Zhiru Xu Guanjun Liu The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination BMC Genomics Poplar Seed germination and post-germination RNA-Seq Metabolite changes Transcriptional timetable Physiological index |
| title | The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination |
| title_full | The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination |
| title_fullStr | The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination |
| title_full_unstemmed | The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination |
| title_short | The transcriptional events and their relationship to physiological changes during poplar seed germination and post-germination |
| title_sort | transcriptional events and their relationship to physiological changes during poplar seed germination and post germination |
| topic | Poplar Seed germination and post-germination RNA-Seq Metabolite changes Transcriptional timetable Physiological index |
| url | http://link.springer.com/article/10.1186/s12864-019-6180-5 |
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