Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation
The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6681 |
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| author | Agnieszka Szopa Michał Dziurka Paweł Kubica Karolina Jafernik Oliwia Siomak Halina Ekiert |
| author_facet | Agnieszka Szopa Michał Dziurka Paweł Kubica Karolina Jafernik Oliwia Siomak Halina Ekiert |
| author_sort | Agnieszka Szopa |
| collection | DOAJ |
| description | The study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) <i>Schisandra rubriflora</i> Rehd. et Wils. (Schisandraceae) lines. The elicitors were supplemented on the 10th day of culture. Biomasses were collected at 24 h and 48 h, and 4, 6, and 8 days after the addition of each elicitor. The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC–MS/MS method. The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h). In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW). The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures. The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of <i>S. rubriflora</i> emphasize the innovativeness of the research. |
| first_indexed | 2024-03-09T21:24:07Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:24:07Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-d3e541a7043c4eaabd76d676dbb545612023-11-23T21:15:28ZengMDPI AGMolecules1420-30492022-10-012719668110.3390/molecules27196681Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by ElicitationAgnieszka Szopa0Michał Dziurka1Paweł Kubica2Karolina Jafernik3Oliwia Siomak4Halina Ekiert5Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, PolandChair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandChair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandChair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandChair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, PolandThe study investigated the effect of elicitation with: chitosan (CH) (200 mg/L), yeast extract (YeE) (3000 mg/L), ethephon (ETH) (25 µM/L), and methyl jasmonate (MeJA) (50 µM/L), on lignan accumulation in agitated and bioreactor (Plantform temporary immersion systems) microshoot cultures of female (F) and male (M) <i>Schisandra rubriflora</i> Rehd. et Wils. (Schisandraceae) lines. The elicitors were supplemented on the 10th day of culture. Biomasses were collected at 24 h and 48 h, and 4, 6, and 8 days after the addition of each elicitor. The 24 compounds from the dibenzocyclooctadiene, aryltetralin, dibenzylbutane, and tetrahydrofuran lignans and neolignans were determined qualitatively and quantitatively in biomass extracts using the UHPLC–MS/MS method. The highest total contents [mg/100 g DW] of lignans were: for CH-95.00 (F, day 6) and 323.30 (M, 48 h); for YeE 104.30 (F, day 8) and 353.17 (M, day 4); for ETH 124.50 (F, 48 h) and 334.90 (M, day 4); and for MeJA 89.70 (F, 48 h) and 368.50 (M, 24 h). In the biomass extracts of M cultures grown in bioreactors, the highest total lignan content was obtained after MeJA elicitation (153.20 mg/100 g DW). The maximum total lignan contents in the biomass extracts from agitated and bioreactor cultures were 3.29 and 1.13 times higher, respectively, than in the extracts from the non-elicited cultures. The poor understanding of the chemical composition and the lack of studies in the field of plant biotechnology of <i>S. rubriflora</i> emphasize the innovativeness of the research.https://www.mdpi.com/1420-3049/27/19/6681<i>Schisandra rubriflora</i><i>Schisandra chinensis</i>red-flowered Chinese magnolia vineChinese magnolia vinesex lineslignans |
| spellingShingle | Agnieszka Szopa Michał Dziurka Paweł Kubica Karolina Jafernik Oliwia Siomak Halina Ekiert Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation Molecules <i>Schisandra rubriflora</i> <i>Schisandra chinensis</i> red-flowered Chinese magnolia vine Chinese magnolia vine sex lines lignans |
| title | Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation |
| title_full | Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation |
| title_fullStr | Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation |
| title_full_unstemmed | Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation |
| title_short | Stimulation of Lignan Production in <i>Schisandra rubriflora</i> In Vitro Cultures by Elicitation |
| title_sort | stimulation of lignan production in i schisandra rubriflora i in vitro cultures by elicitation |
| topic | <i>Schisandra rubriflora</i> <i>Schisandra chinensis</i> red-flowered Chinese magnolia vine Chinese magnolia vine sex lines lignans |
| url | https://www.mdpi.com/1420-3049/27/19/6681 |
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