A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the f...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-021-00346-z |
| _version_ | 1797643086821588992 |
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| author | Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic |
| author_facet | Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic |
| author_sort | Simon G. P. Funnell |
| collection | DOAJ |
| description | Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells. |
| first_indexed | 2024-03-11T14:09:35Z |
| format | Article |
| id | doaj.art-d3e61c501c474ee4bce5627f7aeb9067 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-11T14:09:35Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-d3e61c501c474ee4bce5627f7aeb90672023-11-02T00:44:03ZengNature Portfolionpj Vaccines2059-01052021-06-01611510.1038/s41541-021-00346-zA cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cellsSimon G. P. Funnell0Babak Afrough1John James Baczenas2Neil Berry3Kevin R. Bewley4Rebecca Bradford5Clint Florence6Yann Le Duff7Mark Lewis8Ryan V. Moriarty9Shelby L. O. Connor10Karen L. Osman11Steven Pullan12Sujatha Rashid13Kevin S. Richards14Kimberly J. Stemple15Ivana Knezevic16PHE Porton Down, National Infection Service, Public Health EnglandPHE Porton Down, National Infection Service, Public Health EnglandWisconsin National Primate Research Center, University of Wisconsin-MadisonDivision of Infectious Disease Diagnostics, National Institute for Biological Standards and ControlPHE Porton Down, National Infection Service, Public Health EnglandBiodefense and Emerging Infections Resources, NIHOffice of Biodefense, Research Resources and Translational Research, Division of Microbiology and Infectious Diseases, NIAID, NIHDivision of Infectious Disease Diagnostics, National Institute for Biological Standards and ControlOffice of Biodefense, Research Resources and Translational Research, Division of Microbiology and Infectious Diseases, NIAID, NIHWisconsin National Primate Research Center, University of Wisconsin-MadisonWisconsin National Primate Research Center, University of Wisconsin-MadisonPHE Porton Down, National Infection Service, Public Health EnglandPHE Porton Down, National Infection Service, Public Health EnglandBiodefense and Emerging Infections Resources, NIHPHE Porton Down, National Infection Service, Public Health EnglandOffice of Biodefense, Research Resources and Translational Research, Division of Microbiology and Infectious Diseases, NIAID, NIHWorld Health OrganizationAbstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.https://doi.org/10.1038/s41541-021-00346-z |
| spellingShingle | Simon G. P. Funnell Babak Afrough John James Baczenas Neil Berry Kevin R. Bewley Rebecca Bradford Clint Florence Yann Le Duff Mark Lewis Ryan V. Moriarty Shelby L. O. Connor Karen L. Osman Steven Pullan Sujatha Rashid Kevin S. Richards Kimberly J. Stemple Ivana Knezevic A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells npj Vaccines |
| title | A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_full | A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_fullStr | A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_full_unstemmed | A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_short | A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |
| title_sort | cautionary perspective regarding the isolation and serial propagation of sars cov 2 in vero cells |
| url | https://doi.org/10.1038/s41541-021-00346-z |
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