Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract
Context: Terminalia muelleri Benth. (Combretaceae), is rich with phenolics that have antioxidant and cytotoxic activities. No screening studies were published before on T. muelleri. Objective: The study focused on isolation and identification of secondary metabolites from aqueous methanol leaf extra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1406531 |
| _version_ | 1818107541384593408 |
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| author | Walaa A. El-Kashak Samir M. Osman Ahmed H. Gaara Sayed A. El-Toumy Tahia K. Mohamed Iñaki Brouard |
| author_facet | Walaa A. El-Kashak Samir M. Osman Ahmed H. Gaara Sayed A. El-Toumy Tahia K. Mohamed Iñaki Brouard |
| author_sort | Walaa A. El-Kashak |
| collection | DOAJ |
| description | Context: Terminalia muelleri Benth. (Combretaceae), is rich with phenolics that have antioxidant and cytotoxic activities. No screening studies were published before on T. muelleri. Objective: The study focused on isolation and identification of secondary metabolites from aqueous methanol leaf extract of T. muelleri and evaluation of its biological activities. Materials and methods: The n-butanol extract was chromatographed on polyamide 6, and eluted with H2O/MeOH mixtures of decreasing polarity, then separated by different chromatographic tools that yielded 10 phenolic compounds. The antioxidant activity of the extract was evaluated by investigating its total phenolic and flavonoid content and DPPH scavenging effectiveness. The extract and the two acylated flavones were evaluated for their anticancer activity towards MCF-7 and PC3 cancer cell lines. Molecular docking study of the acylated flavones was performed against topoisomerase enzyme. Results and discussion: Two acylated flavonoids, apigenin-8-C-(2″-O-galloyl) glucoside 1 and luteolin-8-C-(2″-O-galloyl) glucoside 2, were isolated and identified for the second time in nature, with eight tannins (3–10), from the leaves of T. muelleri. The extract and compound 10 showed the most significant antioxidant activity (IC50 = 3.55 and 6.34 μg/mL), respectively. The total extract and compound 2 demonstrated cytotoxic effect against MCF-7 with IC50 = 29.7 and 45.2 μg/mL respectively, while compound 1 showed cytotoxic effect against PC3 (IC50 = 40.8 μg/mL). The docking study of compounds 1 and 2 confirmed unique binding mode in the active site of human DNA topoisomerase enzyme. Conclusions: Terminalia muelleri is a promising medicinal plant as it possesses high antioxidant activity and moderate cytotoxic activity against MCF-7. |
| first_indexed | 2024-12-11T02:01:06Z |
| format | Article |
| id | doaj.art-d3e9fea62ef9406f83122136ea3cdbb1 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-11T02:01:06Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-d3e9fea62ef9406f83122136ea3cdbb12022-12-22T01:24:30ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512277228410.1080/13880209.2017.14065311406531Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extractWalaa A. El-Kashak0Samir M. Osman1Ahmed H. Gaara2Sayed A. El-Toumy3Tahia K. Mohamed4Iñaki Brouard5National Research CentreOctober-6-UniversityNational Research CentreNational Research CentreNational Research CentreInstituto de Productos Naturales y Agrobiología, La LagunaContext: Terminalia muelleri Benth. (Combretaceae), is rich with phenolics that have antioxidant and cytotoxic activities. No screening studies were published before on T. muelleri. Objective: The study focused on isolation and identification of secondary metabolites from aqueous methanol leaf extract of T. muelleri and evaluation of its biological activities. Materials and methods: The n-butanol extract was chromatographed on polyamide 6, and eluted with H2O/MeOH mixtures of decreasing polarity, then separated by different chromatographic tools that yielded 10 phenolic compounds. The antioxidant activity of the extract was evaluated by investigating its total phenolic and flavonoid content and DPPH scavenging effectiveness. The extract and the two acylated flavones were evaluated for their anticancer activity towards MCF-7 and PC3 cancer cell lines. Molecular docking study of the acylated flavones was performed against topoisomerase enzyme. Results and discussion: Two acylated flavonoids, apigenin-8-C-(2″-O-galloyl) glucoside 1 and luteolin-8-C-(2″-O-galloyl) glucoside 2, were isolated and identified for the second time in nature, with eight tannins (3–10), from the leaves of T. muelleri. The extract and compound 10 showed the most significant antioxidant activity (IC50 = 3.55 and 6.34 μg/mL), respectively. The total extract and compound 2 demonstrated cytotoxic effect against MCF-7 with IC50 = 29.7 and 45.2 μg/mL respectively, while compound 1 showed cytotoxic effect against PC3 (IC50 = 40.8 μg/mL). The docking study of compounds 1 and 2 confirmed unique binding mode in the active site of human DNA topoisomerase enzyme. Conclusions: Terminalia muelleri is a promising medicinal plant as it possesses high antioxidant activity and moderate cytotoxic activity against MCF-7.http://dx.doi.org/10.1080/13880209.2017.1406531dpphantioxidantanticancermolecular docking |
| spellingShingle | Walaa A. El-Kashak Samir M. Osman Ahmed H. Gaara Sayed A. El-Toumy Tahia K. Mohamed Iñaki Brouard Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract Pharmaceutical Biology dpph antioxidant anticancer molecular docking |
| title | Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract |
| title_full | Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract |
| title_fullStr | Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract |
| title_full_unstemmed | Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract |
| title_short | Phenolic metabolites, biological activities, and isolated compounds of Terminalia muelleri extract |
| title_sort | phenolic metabolites biological activities and isolated compounds of terminalia muelleri extract |
| topic | dpph antioxidant anticancer molecular docking |
| url | http://dx.doi.org/10.1080/13880209.2017.1406531 |
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