Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages
In obesity, high levels of saturated fatty acids (SFAs) contribute to adipose tissue inflammation and dysfunction. Obesity-induced macrophage infiltration leads to insulin resistance, but the adipocyte itself may play a role in generating the inflammatory milieu. Given our recent findings of the rol...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Adipocyte |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21623945.2020.1760674 |
| _version_ | 1818670122087219200 |
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| author | K. McKernan M. Varghese R. Patel K. Singer |
| author_facet | K. McKernan M. Varghese R. Patel K. Singer |
| author_sort | K. McKernan |
| collection | DOAJ |
| description | In obesity, high levels of saturated fatty acids (SFAs) contribute to adipose tissue inflammation and dysfunction. Obesity-induced macrophage infiltration leads to insulin resistance, but the adipocyte itself may play a role in generating the inflammatory milieu. Given our recent findings of the role of TLR4 in myeloid biasing in obesity, we next investigated the role of TLR4 in adipocyte generated inflammatory responses to SFAs and lipopolysaccharides. We used WT and Tlr4−/- ear mesenchymal stem cell derived adipocytes (EMSC Ad) and bone marrow dendritic cells (BMDCs) to evaluate cell specific responses. Our work demonstrates a role for TLR4 in adipocyte- immune cell crosstalk and that SFA derived metabolites from adipocytes may induce proinflammatory stimulation of immune cells in a TLR4 independent manner. |
| first_indexed | 2024-12-17T07:03:05Z |
| format | Article |
| id | doaj.art-d3ea736f477f4bcda4837258f9dc9738 |
| institution | Directory Open Access Journal |
| issn | 2162-3945 2162-397X |
| language | English |
| last_indexed | 2024-12-17T07:03:05Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj.art-d3ea736f477f4bcda4837258f9dc97382022-12-21T21:59:15ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2020-01-019121222210.1080/21623945.2020.17606741760674Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophagesK. McKernan0M. Varghese1R. Patel2K. Singer3University of Michigan Medical SchoolUniversity of Michigan Medical SchoolUniversity of Michigan Medical SchoolUniversity of Michigan Medical SchoolIn obesity, high levels of saturated fatty acids (SFAs) contribute to adipose tissue inflammation and dysfunction. Obesity-induced macrophage infiltration leads to insulin resistance, but the adipocyte itself may play a role in generating the inflammatory milieu. Given our recent findings of the role of TLR4 in myeloid biasing in obesity, we next investigated the role of TLR4 in adipocyte generated inflammatory responses to SFAs and lipopolysaccharides. We used WT and Tlr4−/- ear mesenchymal stem cell derived adipocytes (EMSC Ad) and bone marrow dendritic cells (BMDCs) to evaluate cell specific responses. Our work demonstrates a role for TLR4 in adipocyte- immune cell crosstalk and that SFA derived metabolites from adipocytes may induce proinflammatory stimulation of immune cells in a TLR4 independent manner.http://dx.doi.org/10.1080/21623945.2020.1760674toll-like receptorsinflammationadipose tissue macrophagesadipogenesisear mesenchymal stem cellsbone marrow derived dendritic cellslipopolysaccharidesaturated fatty acidscytokines |
| spellingShingle | K. McKernan M. Varghese R. Patel K. Singer Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages Adipocyte toll-like receptors inflammation adipose tissue macrophages adipogenesis ear mesenchymal stem cells bone marrow derived dendritic cells lipopolysaccharide saturated fatty acids cytokines |
| title | Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages |
| title_full | Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages |
| title_fullStr | Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages |
| title_full_unstemmed | Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages |
| title_short | Role of TLR4 in the induction of inflammatory changes in adipocytes and macrophages |
| title_sort | role of tlr4 in the induction of inflammatory changes in adipocytes and macrophages |
| topic | toll-like receptors inflammation adipose tissue macrophages adipogenesis ear mesenchymal stem cells bone marrow derived dendritic cells lipopolysaccharide saturated fatty acids cytokines |
| url | http://dx.doi.org/10.1080/21623945.2020.1760674 |
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