Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost
Daptomycin remains as one of the main treatment options for Methicillin-Resistant Staphylococcus aureus (MRSA). Sporadic resistance cases reported in patients treated with either daptomycin or glycopeptides are a growing concern. In a previous study, we described a clinical case of a patient with a...
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02303/full |
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| author | Melanie Roch Paula Gagetti Paula Gagetti James Davis James Davis Paola Ceriana Laura Errecalde Alejandra Corso Adriana E. Rosato |
| author_facet | Melanie Roch Paula Gagetti Paula Gagetti James Davis James Davis Paola Ceriana Laura Errecalde Alejandra Corso Adriana E. Rosato |
| author_sort | Melanie Roch |
| collection | DOAJ |
| description | Daptomycin remains as one of the main treatment options for Methicillin-Resistant Staphylococcus aureus (MRSA). Sporadic resistance cases reported in patients treated with either daptomycin or glycopeptides are a growing concern. In a previous study, we described a clinical case of a patient with a community-acquired MRSA infection resistant to daptomycin and with intermediate resistance to vancomycin who developed a recurrent infection with a susceptible isogenic strain. In the present work, we further investigated the sequential events to determine whether the switch from a daptomycin resistance to a susceptible phenotype was due to a phenomenon of resistance reversion or recurrent infection with a susceptible strain. Pairwise competition experiments showed that the susceptible clinical recurrent SA6850 strain had increased fitness when compared to the resistant counterpart SA6820 strain. In fact, although we have demonstrated that reversion of daptomycin resistance to daptomycin susceptible can occur in vitro after serial passages in drug-free media, phylogenetic analysis suggested that the in vivo process was the result of a recurrent infection with a previous susceptible isolate carried by the patient rather than a resistance reversion of the strain. Whole genome sequence of evolved strains showed that daptomycin resistance in MRSA is associated with a high fitness cost mediated by mutations in mprF gene, revealed as a key element of the biological cost. Moreover, we determined that daptomycin resistance-associated fitness cost was independent of vancomycin intermediate resistance phenotype, as demonstrated in additional clinical MRSA vancomycin susceptible strains. This study highlights important observations as, despite daptomycin offers a useful treatment option for the patients with persistent infections, it has to be carefully monitored. The high fitness cost associated to daptomycin resistance may explain the reduced dissemination of daptomycin resistance and the absence of daptomycin reported outbreaks. |
| first_indexed | 2024-12-23T14:33:37Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T14:33:37Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d3ec62596b924d0ca3c079e9de5edf012022-12-21T17:43:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-12-01810.3389/fmicb.2017.02303309849Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness CostMelanie Roch0Paula Gagetti1Paula Gagetti2James Davis3James Davis4Paola Ceriana5Laura Errecalde6Alejandra Corso7Adriana E. Rosato8Department of Pathology and Genomic Medicine, Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, Houston, TX, United StatesDepartment of Pathology and Genomic Medicine, Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, Houston, TX, United StatesServicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, Buenos Aires, ArgentinaArgonne National Laboratory (DOE), Lemont, IL, United StatesComputation Institute, University of Chicago, Chicago, IL, United StatesServicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, Buenos Aires, ArgentinaDepartamento de Microbiologia, Hospital General de Agudos Juan Fernandez, Buenos Aires, ArgentinaServicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, Buenos Aires, ArgentinaDepartment of Pathology and Genomic Medicine, Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, Houston, TX, United StatesDaptomycin remains as one of the main treatment options for Methicillin-Resistant Staphylococcus aureus (MRSA). Sporadic resistance cases reported in patients treated with either daptomycin or glycopeptides are a growing concern. In a previous study, we described a clinical case of a patient with a community-acquired MRSA infection resistant to daptomycin and with intermediate resistance to vancomycin who developed a recurrent infection with a susceptible isogenic strain. In the present work, we further investigated the sequential events to determine whether the switch from a daptomycin resistance to a susceptible phenotype was due to a phenomenon of resistance reversion or recurrent infection with a susceptible strain. Pairwise competition experiments showed that the susceptible clinical recurrent SA6850 strain had increased fitness when compared to the resistant counterpart SA6820 strain. In fact, although we have demonstrated that reversion of daptomycin resistance to daptomycin susceptible can occur in vitro after serial passages in drug-free media, phylogenetic analysis suggested that the in vivo process was the result of a recurrent infection with a previous susceptible isolate carried by the patient rather than a resistance reversion of the strain. Whole genome sequence of evolved strains showed that daptomycin resistance in MRSA is associated with a high fitness cost mediated by mutations in mprF gene, revealed as a key element of the biological cost. Moreover, we determined that daptomycin resistance-associated fitness cost was independent of vancomycin intermediate resistance phenotype, as demonstrated in additional clinical MRSA vancomycin susceptible strains. This study highlights important observations as, despite daptomycin offers a useful treatment option for the patients with persistent infections, it has to be carefully monitored. The high fitness cost associated to daptomycin resistance may explain the reduced dissemination of daptomycin resistance and the absence of daptomycin reported outbreaks.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02303/fullMRSAdaptomycinbiological costin vivo |
| spellingShingle | Melanie Roch Paula Gagetti Paula Gagetti James Davis James Davis Paola Ceriana Laura Errecalde Alejandra Corso Adriana E. Rosato Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost Frontiers in Microbiology MRSA daptomycin biological cost in vivo |
| title | Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost |
| title_full | Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost |
| title_fullStr | Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost |
| title_full_unstemmed | Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost |
| title_short | Daptomycin Resistance in Clinical MRSA Strains Is Associated with a High Biological Fitness Cost |
| title_sort | daptomycin resistance in clinical mrsa strains is associated with a high biological fitness cost |
| topic | MRSA daptomycin biological cost in vivo |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02303/full |
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